LOCUS       sequence-177800-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5 tagged triple mutant yki in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Oh H, Irvine KD
  TITLE     In vivo analysis of Yorkie phosphorylation sites.
  JOURNAL   Oncogene. 2009 Apr 30;28(17):1916-27. doi: 10.1038/onc.2009.43. Epub
            2009 Mar 30.
  PUBMED    19330023
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2009.43"; journalName: "Oncogene"; date: "2009-04-30- 
            30"; volume: "28"; issue: "17"; pages: "1916-27"
COMMENT     Sequence Label: pUAST-attB-yki:V5-S111A, S168A, S250A
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    950..983
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1016..1110
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1129..1367
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1314..1335
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2751..2792
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2802..2819
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          2909..2974
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3104..3124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3396..3530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3433..3452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3487..3506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3559..3628
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3847..3865)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3886..3902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3886..3902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3899..3921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3910..3926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3934..3940,3941..3958,3959..3964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3979..4000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4117..4134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4288..4876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4368..4387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5047..5838,5839..5907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5670..5689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5908..6012)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6038..6493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6175..6196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6387..6406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6620..6642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6634..6651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6635..6651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6658..6677
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6658..6676
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6710..6728)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7180..7251,7653..7926,8001..8655,8717..9032,9236..9367,
                     9438..9877,1..175)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
       61 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
      121 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
      181 atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt
      241 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
      301 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
      361 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
      421 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
      481 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
      541 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
      601 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
      661 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
      721 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
      781 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
      841 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
      901 aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta
      961 taatgtatgc tatacgaagt tatgctagcg gatccaagct tgcatgcctg caggtcggag
     1021 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     1081 tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa
     1141 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
     1201 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa
     1261 gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta
     1321 ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg
     1381 gaattcgtta acagatctgc ggccgcggct cgagggtacc tactagtcca gtgtggtgga
     1441 attctgcaga tatgtgcgcg tgcctaatcg ctaagataat tctatgtagt tttcgtttgt
     1501 atacaataag tgccttttat atgttaacga cgatgtcagc cagcagcaat acaaacagcc
     1561 tgatcgagaa ggagatcgac gacgaggaca tgctttcgcc gatcaagtcc aacaacctgg
     1621 tggtgcgggt caaccaggac acggacgaca acctgcaggc gctattcgac agcgtcctga
     1681 atccgggtga cgccaagcgc ccgctgcagc tgcccctgcg catgcggaag ctgcccaact
     1741 ccttcttcac gcccccggcg ccctcgcact cgcgggccaa cgccgccgac tccacctacg
     1801 acgcgggctc ccagtcgagc atcaacatcg ggaacaaggc gtccatcgtc cagcagccag
     1861 atggccagtc gcccatcgcc gccatccccc agctccagat tcagccgtct ccccagcaca
     1921 gccgcctggc gatacatcac tcccgagccc gcgccagccc cgcctcgctg cagcagaact
     1981 acaatgtgcg cgcccggagc gacgcagcag cagccaacaa tccgaatgcc aatccgagca
     2041 gccaacagca gcccgctggg cccactttcc cagagaacag tgcccaagag ttccccagcg
     2101 gcgccccggc cagctcggcc attgatctgg acgccatgaa cacctgcatg tcgcaggaca
     2161 ttcccatgtc catgcagaca gtgcacaaga agcagcgcgc ctacgacgtc atcagcccca
     2221 ttcagttgaa ccgccaacta ggcgccttgc cgccgggatg ggagcaagcc aagaccaatg
     2281 atggccagat ctactacttg aatcatacta caaaatctac gcagtgggag gatcccagaa
     2341 tccaatatcg ccagcagcag caaatcttga tggccgagcg aataaagcag aatgatgttt
     2401 tgcaaactac aaaacaaact accacatcga ccattgctaa caatttgggt ccactgccgg
     2461 atggttggga gcaggcagtt accgagtccg gagatcttta ctttataaat cacattgatc
     2521 gaacgacttc atggaatgat cccagaatgc aatctgggct tagcgtgctc gactgcccag
     2581 ataacttagt gtcttccctc cagattgagg ataatctttg cagtaacttg ttcaatgacg
     2641 cacaggccat tgtaaatccg ccgtcttccc acaaacctga cgatttggaa tggtataaaa
     2701 ttaattatcc agcacagtgg cggccgctcg agtctagagg gcccttcgaa ggtaagccta
     2761 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
     2821 gagtttctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     2881 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     2941 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     3001 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     3061 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     3121 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     3181 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     3241 atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca
     3301 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3361 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cactagtgtc
     3541 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     3601 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     3661 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     3721 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg
     3781 cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt
     3841 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     3901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6001 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     6061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     6121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     6361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     6421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     6481 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6541 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6601 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     6661 tacgactcac tatagggcga attgggtacg taccgggccc ctagtatgta tgtaagttaa
     6721 taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga
     6781 tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac
     6841 caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg
     6901 cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt
     6961 tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc
     7021 ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca
     7081 aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt
     7141 tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag
     7201 ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt
     7261 acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt
     7321 gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata
     7381 gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc
     7441 atatcatgat caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa
     7501 tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa
     7561 cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg
     7621 tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct
     7681 gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc
     7741 cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca
     7801 atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc
     7861 ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca
     7921 agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca
     7981 attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat
     8041 gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca
     8101 gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa
     8161 ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac
     8221 ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc tgacctatcg
     8281 gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca
     8341 cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct
     8401 ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg agcccacctc
     8461 cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa
     8521 gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga
     8581 caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt
     8641 cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat tacataacat
     8701 ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt
     8761 acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca
     8821 agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag ttgttggcca
     8881 ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct
     8941 ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac
     9001 tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat
     9061 ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt gcctgattcc
     9121 acacccttct tagttttttt caatgagatg tatagtttat agttttgcag aagataaata
     9181 aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt
     9241 tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa
     9301 tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac
     9361 gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta actttctaat
     9421 gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa
     9481 gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttgccgcttt
     9541 ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg
     9601 gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt
     9661 ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc
     9721 cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc
     9781 cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc
     9841 tgattaacca atgggcggac gtggagccgg gcgaaat
//
