LOCUS       sequence-177801-        9307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP and V5 in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD
  TITLE     Zyxin links fat signaling to the hippo pathway.
  JOURNAL   PLoS Biol. 2011 Jun;9(6):e1000624. doi: 
            10.1371/journal.pbio.1000624. Epub 2011 Jun 7.
  PUBMED    21666802
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-06"; volume: "9(6):e1000624. doi"; pages: " 
            10.1371/journal.pbio.1000624. Epub 2011 Jun 7"
COMMENT     Sequence Label: pUAST-attB-GFP-V5-His
FEATURES             Location/Qualifiers
     source          1..9307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..254
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     201..222
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(351..379)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     974..994
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1068..1109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1119..1136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          1226..1291
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1421..1441
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1713..1847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1750..1769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1804..1823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1876..1945
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2164..2182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2203..2219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2203..2219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2227..2243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2251..2257,2258..2275,2276..2281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2296..2317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3364..4155,4156..4224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3987..4006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4225..4329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4355..4810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4492..4513)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4704..4723
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4937..4959
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4951..4968
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4952..4968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4975..4994
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4975..4993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5027..5045)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5497..5568,5970..6243,6318..6972,7034..7349,7553..7684,
                     7755..8369)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9144..9177
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9210..9304
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
       61 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      121 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      181 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      241 agaagagaac tctgaatagg gaattgggaa ttcgttaaca gatctgcggc cgcggctcga
      301 gggtaccggt agaaaaaatg gctagcaaag gagaagaact tttcactgga gttgtcccaa
      361 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      421 aaggtgatgc tacatacgga aagcttaccc ttaaatttat ttgcactact ggaaaactac
      481 ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt
      541 atccggatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      601 aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt
      661 ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg
      721 gaaacattct cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg
      781 cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgtcacaac attgaagatg
      841 gatccgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      901 ttttaccaga caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa
      961 agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg
     1021 atgatccagc acagtggcgg ccgctcgagt ctagagggcc cttcgaaggt aagcctatcc
     1081 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     1141 tttctagagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     1201 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     1261 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     1321 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     1381 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     1441 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     1501 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     1561 caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc
     1621 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1681 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccac tagtgtcgac
     1861 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
     1921 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
     1981 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
     2041 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
     2101 cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca ctagttctag ccagcttttg
     2161 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
     2221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2281 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2341 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4321 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     4381 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4441 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4501 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4561 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4621 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4681 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4741 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4801 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4861 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4921 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     4981 gactcactat agggcgaatt gggtacgtac cgggccccta gtatgtatgt aagttaataa
     5041 aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat
     5101 cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag
     5161 gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct
     5221 ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg
     5281 ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt
     5341 ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa
     5401 ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg
     5461 gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta
     5521 ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca
     5581 tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc
     5641 tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg
     5701 acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata
     5761 tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat
     5821 aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct
     5881 acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag
     5941 ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca
     6001 ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg
     6061 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     6121 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     6181 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     6241 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     6301 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     6361 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     6421 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     6481 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     6541 cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca
     6601 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     6661 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     6721 attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     6781 actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     6841 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     6901 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     6961 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc
     7021 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     7081 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     7141 tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     7201 aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     7261 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     7321 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     7381 acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca
     7441 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat
     7501 ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     7561 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat
     7621 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     7681 aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa
     7741 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     7801 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     7861 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     7921 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     7981 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     8041 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     8101 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     8161 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     8221 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     8281 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     8341 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     8401 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     8461 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     8521 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     8581 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     8641 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     8701 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     8761 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     8821 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     8881 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     8941 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     9001 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     9061 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     9121 gatttcactg gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct
     9181 agcggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     9241 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     9301 cgagcgg
//
