LOCUS       sequence-177848-        9374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Mb3Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9374)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY117 (pcDNA3.1-huMb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..9374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             935..955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4760..4807
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4814..4894
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4968..4985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4974..5198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5244..5672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5331..5350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5541..5562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5681..5701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5686..6015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5866..6001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5928..5947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6082..6876
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6136..6155)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6746..6765
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7050..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7141..7160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7220..7236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7220..7236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7233..7255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7244..7260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7268..7274,7275..7292,7293..7298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7313..7334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7451..7468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7622..8207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7702..7721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8378..9169,9170..9238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9001..9020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9239..9343)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttaccg gtgccaccat ggccccaaag aagaagcgga aggtcggtat
      961 ccacggagtc ccagcagccc tgttccagga cttcacccac ctgtaccccc tgagcaagac
     1021 cgtcagattc gagctgaagc ccatcggcaa gaccctggaa cacatccacg ccaagaactt
     1081 tctgaaccag gacgagacaa tggccgacat gtaccagaaa gtgaaggcca tcctggacga
     1141 ctaccaccgg gactttatcg ccgacatgat gggcgaagtg aagctgacca agctggccga
     1201 gttctacgac gtgtacctga agttccggaa gaaccccaag gacgacggcc tgcagaagca
     1261 gctgaaagac ctgcaggctg tgctgcggaa agaaatcgtg aagcctatcg gaaacggcgg
     1321 caagtacaag gccggctacg acagactgtt cggcgccaag ctgttcaagg acggcaaaga
     1381 gctgggcgac ctggccaagt tcgtgatcgc ccaggaaggc gagagcagcc ctaagctggc
     1441 ccacctggcc catttcgaga agttcagcac ctacttcacc ggcttccacg acaaccgcaa
     1501 gaacatgtac agcgacgagg acaagcacac cgccattgcc taccggctga tccacgagaa
     1561 cctgccccgg ttcatcgaca acctgcagat cctggccacc atcaagcaga agcacagcgc
     1621 cctgtacgac cagatcatca acgagctgac cgccagcggc ctggatgtgt ctctggcctc
     1681 tcacctggat ggctaccaca agctgctgac acaggaaggc atcaccgcct acaacaccct
     1741 gctgggcgga atctctggcg aggccggcag cagaaagatc cagggcatca atgaactgat
     1801 caacagccac cacaaccagc actgccacaa gagcgagcgg attgccaagc tgcggcccct
     1861 gcacaagcag attctgagcg acggaatggg cgtgtccttc ctgcccagca agttcgccga
     1921 cgacagcgaa gtgtgccagg ccgtgaatga gttctaccgg cactacgccg acgtgttcgc
     1981 caaggtgcag agcctgttcg acggcttcga cgactatcag aaagacggca tctacgtgga
     2041 gtacaagaac ctgaacgagc tgtccaagca ggccttcggc gacttcgctc tgctgggaag
     2101 agtgctggac ggctactatg tggacgtcgt gaaccccgag ttcaacgaga gatttgccaa
     2161 ggccaagacc gacaacgcca aagccaagct gacaaaagag aaggacaagt tcatcaaggg
     2221 cgtgcactcc ctggcttctc tggaacaggc catcgagcac tacaccgcca gacacgacga
     2281 cgagtctgtg caggccggaa agctgggcca gtacttcaag cacggactgg ccggcgtgga
     2341 caaccccatc cagaagatcc acaacaacca ctctacaatc aagggcttcc tggaaagaga
     2401 gcggcctgcc ggcgaaagag ccctgcccaa gatcaagagc gacaagagcc ccgagatcag
     2461 acagctgaaa gagctgctgg acaacgccct gaacgtggcc cacttcgcca aactgctgac
     2521 caccaagacc acactgcaca atcaggatgg caacttctac ggcgagttcg gagccctgta
     2581 tgatgagctg gccaagatcg ccaccctgta caacaaagtg cgggactacc tgagccagaa
     2641 gcccttcagc accgagaagt acaagctgaa tttcggcaac cctaccctgc tgaacggctg
     2701 ggacctgaac aaagagaaag ataacttcgg cgtgatcctg cagaaggacg gctgctacta
     2761 cctggccctg ctggataagg cccacaagaa ggtgttcgat aacgccccca acaccggcaa
     2821 gtctgtgtat cagaagatga tctataagct gctgcctggc cccaacaaga tgctgcccaa
     2881 ggtgttcttc gccaagagca acctggacta ctacaacccc agcgccgaac tgctggacaa
     2941 atacgcccag ggcacacaca agaaaggcga caacttcaac ctgaaggact gccacgccct
     3001 gatcgatttc ttcaaggccg gaatcaacaa gcaccccgag tggcagcact tcggcttcaa
     3061 gttcagcccc accagcagct accaggacct gagcgacttc tacagagagg tggaacccca
     3121 gggctaccaa gtgaagttcg tggacatcaa cgccgactac atcaatgagc tggtggaaca
     3181 gggccagctg tacctgtttc agatctacaa caaggacttt agccccaagg cccacggcaa
     3241 gcccaacctg cacaccctgt atttcaaggc cctgtttagc gaggacaacc tcgtgaatcc
     3301 catctacaaa ctgaacgggg aggccgagat cttctaccgg aaggcctccc tggacatgaa
     3361 cgagacaacc atccacagag ccggcgaggt gctggaaaac aagaaccctg acaaccctaa
     3421 gaaacggcag ttcgtgtacg acatcatcaa ggacaagcgg tacacccagg ataagttcat
     3481 gctgcacgtg cccatcacca tgaacttcgg agtgcagggc atgaccatca aagagttcaa
     3541 caaaaaagtg aaccagagca tccagcagta cgacgaagtg aacgtgatcg gcatcgaccg
     3601 gggcgagcgg catctgctgt atctgaccgt gatcaactcc aagggcgaga ttctggaaca
     3661 gagatccctg aacgacatca ccaccgcctc cgccaacggc acccagatga ccacccctta
     3721 ccacaagatc ctggacaagc gcgagatcga gcggctgaac gccagagtgg gatggggaga
     3781 gatcgagaca atcaaagaac tgaagtccgg ctacctgtcc cacgtggtgc atcagatctc
     3841 ccagctgatg ctgaagtaca acgccatcgt ggtgctggaa gatctgaatt ttggcttcaa
     3901 gaggggccgg ttcaaggtgg aaaagcagat ctaccagaac ttcgagaatg ccctgatcaa
     3961 gaaactgaac cacctggtgc tgaaagacaa ggccgacgac gagatcggca gctacaagaa
     4021 cgccctgcag ctgactaaca acttcaccga tctgaagagt atcggcaagc agaccggctt
     4081 tctgttctac gtgcccgcct ggaataccag caagatcgac cccgagacag gcttcgtgga
     4141 cctgctgaag cctagatacg agaatatcgc ccagagccag gccttcttcg gcaagttcga
     4201 caagatctgc tacaacgccg ataggggcta cttcgagttc cacatcgact acgccaagtt
     4261 caatgacaag gccaaaaaca gccggcagat ctggaaaatc tgcagccacg gcgataagcg
     4321 ctacgtgtac gataagaccg ccaaccagaa caagggcgcc accatcggag tgaatgtgaa
     4381 cgatgagctg aagtccctgt tcacccgcta ccacatcaac gataagcagc ccaatctcgt
     4441 gatggacatc tgccagaaca acgacaaaga gtttcacaag agcctgatgt atctgctgaa
     4501 aacactgctg gctctgcggt acagcaacgc cagctccgac gaggatttca tcctgagccc
     4561 cgtggccaac gacgagggcg tgttcttcaa tagcgccctg gccgacgata cccagcccca
     4621 gaatgccgat gccaacggcg cctaccacat tgccctgaag ggactgtggc tgctgaacga
     4681 actgaagaac agcgacgatc tgaacaaagt gaagctggcc atcgacaacc agacctggct
     4741 gaatttcgcc cagaacagaa agaggccggc ggccacgaag aaggccggcc aggccaagaa
     4801 gaagaaggga tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga
     4861 ttatgcatac ccatatgatg tccccgacta tgcctaagaa ttctgcagat atccagcaca
     4921 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4981 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5041 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5101 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5161 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5221 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5581 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5641 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5761 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5821 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5941 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6001 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6061 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6121 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6181 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6241 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6301 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6361 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6421 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6481 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6541 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6601 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6661 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6721 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6781 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6841 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     6901 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6961 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     7021 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     7201 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7261 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7321 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8041 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     8161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9301 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9361 tgccacctga cgtc
//
