LOCUS       sequence-177849-        9263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Lb4Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9263)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY118 (pcDNA3.1-huLb4Cpf1)
FEATURES             Location/Qualifiers
     source          1..9263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             935..955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4649..4696
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4703..4783
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4863..5087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5133..5561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5570..5590)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5575..5904
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5755..5890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5817..5836
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5971..6765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6025..6044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6635..6654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6939..7060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6976..6995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7030..7049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7109..7125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7109..7125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7122..7144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7157..7163,7164..7181,7182..7187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7340..7357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7511..8096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7591..7610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8267..9058,9059..9127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8890..8909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9128..9232)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttaccg gtgccaccat ggccccaaag aagaagcgga aggtcggtat
      961 ccacggagtc ccagcagccg gcctgtacga cggcttcgtg aacagataca gcgtgtccaa
     1021 gaccctgaga ttcgagctga tcccccaggg ccggaccaga gagtacatcg agacaaacgg
     1081 catcctgagc gacgacgagg aacgggccaa ggactacaag accatcaagc ggctgatcga
     1141 cgagtaccac aaggattaca tcagccggtg cctgaagaac gtgaacatca gctgcctgga
     1201 agagtactac cacctgtaca acagcagcaa ccgggacaag cggcacgagg aactggacgc
     1261 cctgtccgac cagatgagag gcgagatcgc cagcttcctg accggcaacg atgagtacaa
     1321 agagcagaag tcccgcgaca tcatcatcaa cgagcggatc atcaacttcg ccagcaccga
     1381 cgaagaactg gccgccgtga agcggttccg gaagttcacc agctacttca ccggcttttt
     1441 caccaaccgc gagaacatgt acagcgccga gaagaagtcc accgccattg cccaccggat
     1501 catcgacgtg aacctgccca aatacgtgga caacatcaag gccttcaaca ccgccatcga
     1561 ggccggcgtg ttcgatatcg ccgagttcga gagcaacttc aaggccatca ccgatgagca
     1621 cgaggtgtcc gacctgctgg acatcaccaa gtacagccgg ttcatccgga acgaggatat
     1681 catcatctac aacaccctgc tgggcggcat cagcatgaag gacgagaaga tccagggcct
     1741 gaatgagctg atcaacctgc acaaccagaa gcaccccggc aagaaggtgc ccctgctgaa
     1801 ggtgctgtac aagcagatcc tgggcgacag ccagacccac agcttcgtgg acgaccagtt
     1861 cgaggacgat cagcaagtga tcaatgccgt gaaggccgtg accgacacct tctccgagac
     1921 actgctgggc agcctgaaga tcatcattaa caacatcggc cactacgacc tggaccggat
     1981 ctatatcaag gccggccagg atatcaccac cctgagcaag agagccctga acgactggca
     2041 catcatcacc gagtgcctgg aaagcgagta cgacgacaag ttccccaaga acaagaagtc
     2101 cgacacctac gaggaaatgc gcaacagata cgtgaagtcc ttcaagagct tcagcatcgg
     2161 ccggctgaac agcctcgtga ccacctatac cgagcaggcc tgtttcctgg aaaactacct
     2221 gggcagcttc ggcggcgaca ccgacaagaa ttgcctgacc gacttcacca acagcctgat
     2281 ggaagtggaa catctgctga actccgagta ccccgtgacc aatcggctga tcaccgacta
     2341 cgagagcgtg cgcatcctga agcggctgct ggatagcgaa atggaagtga tccactttct
     2401 gaagcctctg ctgggaaacg gcaacgagag cgacaaggac ctggtgttct acggcgagtt
     2461 tgaggccgag tacgagaagc tgctgcccgt gatcaaggtg tacaatagag tgcggaacta
     2521 cctgacccgg aagcccttta gcacagagaa gatcaagctg aacttcaaca gccctaccct
     2581 gctgtgtggc tggtcccaga gcaaagaaaa agagtacatg ggcgtgatcc tgcggaagga
     2641 cggccagtac tatctgggca tcatgacccc cagcaacaag aagatcttta gcgaggcccc
     2701 caagcccgac gaggactgct atgagaagat ggtgctgcgg tacatccccc acccctacca
     2761 gatgctgccc aaggtgttct tcagcaagag caatatcgcc ttcttcaacc ccagcgacga
     2821 gatcctgaga atcaagaagc aggaatcctt taagaagggc aagagcttta accgggacga
     2881 ctgccacaag ttcatcgact tctacaagga ctccatcaac cggcacgaag agtggcgcaa
     2941 gttcaatttc aagttcagcg ataccgatag ctacgaggac atctcccggt tctacaaaga
     3001 ggtggaaaac caggccttca gcatgagctt caccaagatc cccaccgtgt acatcgacag
     3061 cctggtggac gagggcaagc tgtacctgtt taagctgcac aacaaggatt tcagcgagca
     3121 cagcaagggc aagcccaatc tgcacacagt gtactggaat gccctgttca gcgagtacaa
     3181 cctgcagaat accgtgtacc agctgaacgg ctccgccgag atctttttca gaaaggccag
     3241 catccccgag aacgagcgcg tgatccacaa gaaaaacgtg cccatcaccc ggaaggtggc
     3301 cgagctgaat ggcaagaaag aagtgtccgt gttcccctac gacatcatta agaaccggcg
     3361 gtacaccgtg gataagttcc agtttcacgt gcccctgaag atgaatttca aggccgatga
     3421 gaagaagcgg atcaacgacg acgtgatcga ggccatcaga agcaacaagg gcatccacgt
     3481 gatcggcatc gaccggggcg agcggaatct gctgtacctg agcctgatca acgaggaagg
     3541 cagaatcatc gagcagcggt ccctgaacat catcgactcc ggcgagggcc acacccagaa
     3601 ctacagagat ctgctggatt cccgcgagaa ggacagagag aaggcccggg aaaactggca
     3661 ggaaattcag gaaatcaagg atctgaaaac cggctacctg tcccaggcca tccacaccat
     3721 cacaaagtgg atgaaggagt acaacgccat catcgtgctg gaagatctga acgacagatt
     3781 caccaacggc cggaaaaaag tggaaaaaca ggtgtaccag aaattcgaga agatgctgat
     3841 cgataagctg aattactatg tggacaagga cgaagagttc gaccggatgg gcggcaccca
     3901 tagagccctg cagctgaccg agaagttcga gtccttccag aagctgggcc ggcagaccgg
     3961 cttcatcttc tacgtgcccg cctggaacac cagcaagctg gaccctacca ccggattcgt
     4021 ggacctgctg tatcccaagt acaagagcgt ggacgccacc aaggacttta tcaagaagtt
     4081 tgacttcatc cggttcaaca gcgagaagaa ctacttcgag ttcggcctgc actactccaa
     4141 cttcaccgag cgggccatcg gctgccggga cgagtggatt ctgtgcagct acggcaaccg
     4201 gatcgtgaac ttcagaaacg ccgccaaaaa caacagctgg gattacaaag agatcgacat
     4261 taccaaacag ctgctggacc tgtttgagaa gaacggcatc gatgtgaaac aggaaaacct
     4321 gatcgactcc atctgcgaga tgaaggacaa gccattcttc aagtccctga tcgccaacat
     4381 taagctgatc ctgcagatcc ggaacagcgc cagcggcacc gacatcgact acatgatcag
     4441 ccccgccatg aacgatcggg gcgagttctt cgacaccaga aagggcctgc agcagctgcc
     4501 cctggacgcc gatgctaacg gcgcctacaa cattgccaag aaaggcctgt ggatcgtgga
     4561 ccagatcaga aataccaccg gcaacaacgt gaagatggcc atgagcaaca gagaatggat
     4621 gcactttgcc caggaaagcc ggctggccaa gaggccggcg gccacgaaga aggccggcca
     4681 ggccaagaag aagaagggat cctacccata cgatgttcca gattacgctt atccctacga
     4741 cgtgcctgat tatgcatacc catatgatgt ccccgactat gcctaagaat tctgcagata
     4801 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5041 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5101 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5821 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5881 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5941 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6001 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6061 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6121 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6181 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6241 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6301 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6361 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6421 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6481 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6541 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6601 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6661 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6721 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6781 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6841 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6901 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6961 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7021 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7081 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8041 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8581 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8881 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9241 cccgaaaagt gccacctgac gtc
//
