LOCUS       sequence-177860-        9284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized BfCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY125 (pcDNA3.1-huBfCpf1)
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             935..955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4670..4717
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4724..4804
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4878..4895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4884..5108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5154..5582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5241..5260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5451..5472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5591..5611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5596..5925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5776..5911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5838..5857
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5992..6786
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6046..6065)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6656..6675
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6960..7081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6997..7016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7051..7070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7130..7146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7130..7146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7143..7165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7154..7170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7178..7184,7185..7202,7203..7208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7223..7244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7361..7378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7532..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7612..7631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8288..9079,9080..9148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8911..8930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttaccg gtgccaccat ggccccaaag aagaagcgga aggtcggtat
      961 ccacggagtc ccagcagcct actacgagag cctgaccaag ctgtacccca tcaagaaaac
     1021 catccggaac gagctggtgc ccatcggcaa gaccctggaa aacatcaaga agaacaacat
     1081 cctggaagcc gacgaggacc ggaagatcgc ctacatcaga gtgaaggcca tcatggacga
     1141 ctaccacaag cggctgatca acgaggccct gagcggcttc gccctgatcg acctggataa
     1201 ggccgccaac ctgtacctga gcagaagcaa gagcgccgac gacatcgaga gcttcagccg
     1261 gttccaggac aagctgcgga aggccattgc caagagactg agagagcacg agaacttcgg
     1321 caagatcggc aacaaggaca tcatccccct gctgcagaag ctgagcgaga acgaggacga
     1381 ttacaacgcc ctggaatcct tcaagaactt ctacacctac ttcgagagct acaacgacgt
     1441 gcggctgaac ctgtacagcg acaaagaaaa gtccagcacc gtggcctacc gcctgatcaa
     1501 tgagaacctg cccagattcc tggacaacat ccgggcctac gacgccgtgc agaaggccgg
     1561 catcacaagc gaggaactga gcagcgaggc ccaggacggc ctgttcctcg tgaacacctt
     1621 caacaacgtg ctgatccagg atggcatcaa cacctacaac gaggatatcg gcaagctgaa
     1681 cgtggccatc aatctgtaca accagaaaaa cgccagcgtg cagggcttca gaaaggtgcc
     1741 caagatgaag gtgctgtaca agcagatcct gagcgaccgg gaagagtcct tcatcgacga
     1801 gttcgagagc gacaccgagc tgctggacag cctggaaagc cactacgcca atctggccaa
     1861 gtacttcggc agcaacaagg tgcagctgct gttcaccgcc ctgcgcgagt ctaagggcgt
     1921 gaacgtgtac gtgaagaacg atatcgccaa gaccagcttc agcaacgtgg tgttcggcag
     1981 ctggtcccgg atcgacgagc tgattaacgg cgagtacgac gacaacaaca atcggaagaa
     2041 ggacgagaag tactacgaca agaggcagaa agagctgaag aaaaacaaga gctacaccat
     2101 cgagaagatc atcaccctga gcaccgagga cgtggacgtg atcggcaagt atatcgagaa
     2161 gctggaaagc gacatcgacg atatccggtt caagggcaag aatttctatg aggccgtgct
     2221 gtgcggccac gaccgcagca agaagctgtc caagaacaag ggcgccgtgg aagccatcaa
     2281 gggctacctg gacagcgtga aggacttcga gcgggacctg aagctgatca atggcagcgg
     2341 ccaggaactg gaaaagaacc tggtggtgta cggcgagcag gaagccgtgc tgagcgaact
     2401 gagcggcatc gacagcctgt acaacatgac ccggaactac ctgacaaaga agcccttcag
     2461 caccgagaag attaagctga acttcaacaa gcccaccttc ctggacggct gggactacgg
     2521 caacgaggaa gcctacctgg gcttcttcat gatcaaagag ggcaactact ttctggccgt
     2581 gatggacgcc aactggaaca aagagttcag aaacatcccc agcgtggaca agagcgactg
     2641 ctacaagaaa gtgatctata agcagatcag cagccccgag aagtccatcc agaatctgat
     2701 ggtcatcgat ggcaagaccg tgaagaagaa cggccggaaa gagaaagaag gcatccacag
     2761 cggcgagaac ctgattctgg aagaactgaa gaacacctac ctgcccaaga agatcaacga
     2821 catccggaag cggcggagct acctgaacgg cgacaccttc tccaagaagg acctgaccga
     2881 gttcatcggc tactacaaac agcgcgtgat cgagtactac aacggctaca gcttctactt
     2941 caagtccgac gacgactacg ccagcttcaa agaatttcag gaagatgtgg gccggcaggc
     3001 ctaccagatc agctatgtgg atgtgcccgt gtccttcgtg gacgacctga tcaacagcgg
     3061 caaactgtac ctgttccggg tgtacaacaa ggatttcagc gagtacagca agggcagact
     3121 gaacctgcac accctgtact tcaagatgct gttcgacgag cgcaacctga agaatgtggt
     3181 gtacaagctg aatggccagg ccgaggtgtt ctaccggccc agctctatca agaaagagga
     3241 actgatcgtg cacagagccg gcgaggaaat caagaacaaa aaccccaaga gagccgccca
     3301 gaagcccacc agaaggctgg actacgacat tgtgaaggac cggcggtaca gccaggataa
     3361 gttcatgctg cacaccagca tcatcatgaa ctttggcgcc gaggaaaatg tgtcctttaa
     3421 cgacatcgtg aacggcgtgc tgcggaatga ggacaaagtg aatgtgatcg gaatcgaccg
     3481 gggcgagcgg aacctgctgt acgtggtcgt gattgacccc gagggaaaga tcctggaaca
     3541 gcggagcctg aactgcatca ccgacagcaa cctggacatc gaaaccgatt accacagact
     3601 gctggacgag aaagagagcg atagaaagat cgccagacgg gactggacca caatcgagaa
     3661 catcaaagaa ctgaaggccg gctacctgag ccaggtggtg cacattgtgg ccgaactggt
     3721 gctgaagtac aacgccatca tctgcctgga agatctgaat ttcggcttca agcggggcag
     3781 acagaaagtg gaaaaacagg tgtaccagaa atttgagaag atgctgattg acaaactgaa
     3841 ttacctcgtg atggataaga gccgggaaca gctgtcccct gagaagatct ctggcgccct
     3901 gaacgctctg cagctgaccc ccgacttcaa gagcttcaag gtgctgggaa agcagaccgg
     3961 catcatctac tacgtgcccg cctacctgac cagcaagatc gaccccatga ccggctttgc
     4021 caatctgttc tacgtgaagt atgagaatgt ggacaaggcc aaagagtttt tcagcaagtt
     4081 cgacagcatc aagtataaca aggacggcaa gaactggaat accaagggct atttcgagtt
     4141 cgccttcgac tacaagaagt tcaccgatag agcctacggc cgggtgtccg agtggaccgt
     4201 gtgtacagtg ggcgagagaa tcatcaagtt caaaaacaaa gagaagaaca atagctacga
     4261 tgacaaagtg atcgatctga ccaacagcct gaaagaactg ttcgactcct acaaagtgac
     4321 ctacgagtct gaggtggacc tgaaagacgc tatcctggcc atcgacgacc ccgccttcta
     4381 ccgcgatctg acaagacggc tgcagcagac cctgcagatg cggaacagct cctgcgacgg
     4441 cagccgggac tacatcatca gccctgtgaa gaactccaag ggcgagttct tctgctccga
     4501 caacaatgac gacaccaccc ccaacgacgc cgacgccaat ggcgccttca atatcgccag
     4561 aaagggcctg tgggtgctga acgagatcag aaacagcgag gaaggctcca agatcaacct
     4621 ggccatgagc aacgcccagt ggctggaata cgcccaggac aacaccatca agaggccggc
     4681 ggccacgaag aaggccggcc aggccaagaa gaagaaggga tcctacccat acgatgttcc
     4741 agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta
     4801 tgcctaagaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
     4861 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4921 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4981 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5041 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5101 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5161 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5221 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5281 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5341 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5401 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5461 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5521 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5581 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5761 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5821 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5881 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5941 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6001 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6061 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6121 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6181 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6241 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6301 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6361 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6421 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6481 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6541 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6601 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6661 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6721 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6781 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6841 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6901 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7081 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8041 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
