LOCUS       sequence-17787-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H,
            Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP,
            Gygi SP, Sinclair DA, Alt FW, Greenberg ME
  TITLE     Stress-dependent regulation of FOXO transcription factors by the 
            SIRT1 deacetylase.
  JOURNAL   Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19.
  PUBMED    14976264
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-03-26"; volume: "303(5666)"; pages: "2011-5. Epub 2004 
            Feb 19"
COMMENT     Sequence Label: Flag-SIRT1 H363Y
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     255..277
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     269..286
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     270..286
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     293..312
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        293..311
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttaagctgca taaacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
       61 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac agatgtgata tggctgatta
      121 tgatcattac ttatctaggt ataggctgcg caactgttgg gaagggcgat cggtgcgggc
      181 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      241 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
      301 ctcactatag ggcgaattga atttagcggc cgcgaattcg cccttgtcta gagtggaaca
      361 attcctgtac ctgcacaatt attacactat gatttgtttg atggatagtt catgtctgtt
      421 acttcctgtt tcacagatat agcttcatta attgcctctt gatcatctcc atcagtccca
      481 aatccagctc ctccagctct ctctggaaca tcaggctcat cttctaagcc attgtagaat
      541 tcttcaattt cactttcatc ctccatgggt tcttctaaac ttggactctg gcatgtccca
      601 ctatcactgt tactgccaca agaactagag gataagacgt catcttcaga gtctgaatat
      661 acctcagcgc tgaagaaagg annannttgt gnnnggcaaa aannaaangt nttggcntna
      721 ccccccncnn actngtngtt gcttngntac ncnatnaggc cagcattttc tcactgttcc
      781 ccccnnngaa gtncatttcn tttttntccc cccncana
//
