LOCUS       sequence-177892-        4210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Streptococci shuttle plasmid for gene expression in 
            streptococci with Pspac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Biswas I, Jha JK, Fromm N
  TITLE     Shuttle expression plasmids for genetic studies in Streptococcus 
            mutans.
  JOURNAL   Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 
            10.1099/mic.0.2008/019265-0.
  PUBMED    18667560
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2008-08"; volume: "154(Pt 8):2275-82. doi"; 
            pages: " 10.1099/mic.0.2008/019265-0"
COMMENT     Sequence Label: pIB167
FEATURES             Location/Qualifiers
     source          1..4210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2902..2913
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4077..4094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4182..4205
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 agcttaagtg attagtcaaa gaatggtgat gacaattgta caggttatca ataaagccat
       61 tgatactctc attcttatcc cgtatttatt aacttctcct agtattagta attatcatgg
      121 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa
      181 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa
      241 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta
      301 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa
      361 aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat tagttcaaca
      421 aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac agtaatattg
      481 acttttaaaa aaggattgat tataatgaag aaagcagaca agtaagcctc ctaaattcac
      541 tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat ttagacaatt
      601 ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt agtataacca
      661 cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat aaattttacc
      721 ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt agaactggtt
      781 acaatagcga cggagagtta ggttattggg ataagttaga gccactttat acaatttttg
      841 atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt
      901 tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa ttgtttccca
      961 aaacacctat acctgaaaat gctttttctc tttctattat tccatggact tcatttactg
     1021 ggtttaactt aaatatcaat aataatagta attaccttct acccattatt acagcaggaa
     1081 aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta catcattctg
     1141 tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg tcagataggc
     1201 ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc
     1261 taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc tccagatcca
     1321 tatccttctt tttctgaacc gacttctcct ttttcgcttc tttattccaa ttgctttatt
     1381 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgactttcgt caggggggct
     1441 tttatttatt caataatccc tcctctcaat aaatctattg ttgtacttaa ttcaacttcc
     1501 atttctctgt atctttcaat acgctctttt aagtccttaa tttctttttt taattcctca
     1561 ttttcagcaa ataactcttt ttctttgttt gtcattttat ttcccccgtt tcagcatcaa
     1621 gaacctttgc ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa
     1681 aggcgctaga gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact
     1741 ccgctcccct aaggcgaata aaagccatta actcttttgt atttaccaaa ttatagtcat
     1801 ccactatatc taaaagtaaa ttcttcaatt ttcttttttg gctttcatca agtgttatat
     1861 agcggtcaat atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat
     1921 tcttagcaat agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat
     1981 caagtatctc aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa
     2041 cgctttctat tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat
     2101 agtgctttcc atttcgtata acatcactac tattccatgt atctttatct tttttttcgt
     2161 ccatatcgtg taaaggactg acagccatag atacgcccaa actctctaat ttttctttcc
     2221 aatcattagg aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt
     2281 taatagccat gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa
     2341 aaacgcgagc tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag
     2401 ctaacgccgc aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt
     2461 tatcttgctc ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt
     2521 aaaaaactcc aatcaaataa ttttataaaa ttagtgtatc actttgtaat cataaaaaca
     2581 acaataaagc tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg
     2641 ttggcgattg aaaaacccct caaacccttg agccagttgg gatagagcgt ttttggcaca
     2701 aaaattggca ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt
     2761 ccccccattt ttttccaaat tccaaatttt tttcaaaaat tttccagcgc taccgctcgg
     2821 caaaattgca agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcaaactcc
     2881 cttgagcctc ctccaaccga aatagaagga cgctgcgctt attatttcat tcagtcatcg
     2941 gctttcataa tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt
     3001 tacgctacga taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa
     3061 aacgtctcag aaacgatttt gagacgtttt aataaaaaat cgaccgaaaa ataaatataa
     3121 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     3181 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     3241 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     3301 tgatgacaca gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga
     3361 tggattttct attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta
     3421 gttcaacaaa cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg
     3481 aaggatgctt aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt
     3541 cttatttaga aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc
     3601 aataataatg actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt
     3661 ggataaatat ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac
     3721 tgatgggccc ctgcagatga attaattcta cacagcccag tccagactat tcggcactga
     3781 aattatgggt gaagtggtca agacctcact aggcacctta aaaatagcgc accctgaaga
     3841 agatttattt gaggtagccc ttgcctacct agcttccaag aaagatatcc taacagcaca
     3901 agagcggaaa gatgttttgt tctacatcca gaacaacctc tgctaaaatt cctgaaaaat
     3961 tttgcaaaaa gttgttgact ttatctacaa ggtgtggcat aatgtgtgga attgtgagcg
     4021 gataacaatt ggatctcttt aagaaggagg atatacaaat ggctagcaga ggatcgcatc
     4081 accatcacca tcacggggcc ggagaccgcg gtcccgaatt cgagctcggt acccggggat
     4141 ccctcgaggt cgacctgcag ggggaccatg gtctcagcgc ttggagccac ccgcagttcg
     4201 aaaaataata
//
