LOCUS       sequence-177914-       14645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S2197F point mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Prp8 retinitis pigmentosa mutants cause defects in the transition 
            between the catalytic steps of splicing.
  JOURNAL   RNA. 2016 May;22(5):793-809. doi: 10.1261/rna.055459.115. Epub 2016 
            Mar 11.
  PUBMED    26968627
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.055459.115"; journalName: "RNA"; date: "2016-05"; 
            volume: "22"; issue: "5"; pages: "793-809"
COMMENT     Sequence Label: pRS313-prp8-S2197F
FEATURES             Location/Qualifiers
     source          1..14645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             957..974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9961..9980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9962..9980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9987..10004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9987..10003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9996..10018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10144..10599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10231..10250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10441..10462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(10859..11521)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(11522..11709)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(11856..11875)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11975..11997
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12035..12053)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    12101..12604
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        12634..12738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12739..12807,12808..13599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12957..12976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13770..14358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14259..14278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14512..14529
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac cgggcccccc
      181 ctcgagactt caaagcatgg tttggtttgg gccaggctta tgctctcctg gacatgcatt
      241 tatattctct ttactacttc cagaaagctt gcactttgaa accttgggat cgtcggattt
      301 ggcaagtatt gggagaatgt tatagtaaga cgggaaataa ggtagaagct ataaaatgct
      361 acaaaagatc cataaaagct tcacaaacgg tcgatcaaaa tacttcaata tattaccggt
      421 tagcgcaact atatgaagaa cttgaagact tgcaagaatg taagaagttc atgatgaaat
      481 gtgtagatgt ggaagaactt ctggaaggta tagtaacaga tgaaaccgtg aaggctaggc
      541 tttggctggc aatatttgag attaaggcag gaaactacca attggcttat gattatgcca
      601 tgggggtatc tagtggaacg tctcaagaga ttgaagaggc tcgtatgctg gctcgggagt
      661 gcagaaggca tatgtagtga agtgaacata cacatagcta ttcgtactaa atgatatgaa
      721 atttttataa atgccaggct atatagctat ttaaagtgac catggcagaa ggatgaaccg
      781 aggtaatacg gctagtacaa aagcaacaaa gttaggaata caatttgaga aacgaagacc
      841 atagaaaata cttgtgcgat tgaacttcct tccaaaaaaa aaatagcgtc aaagaaagat
      901 gagtggacta ccgcccccac ctcctggttt tgaagaggac agcgacttag cacttccacc
      961 accaccacca ccaccgcctg gatacgaaat cgaagaactg gataatccga tggtgccatc
     1021 atcggtaaat gaggatacat tccttccgcc tccaccacct cctccaagca acttcgaaat
     1081 aaacgctgaa gaaattgtgg acttcacatt accaccgcca ccaccccctc caggtctaga
     1141 tgaattagag actaaagcag aaaagaaagt agaattacat ggaaagagaa aattggacat
     1201 aggaaaagat actttcgtca ctcgaaaatc aagaaagcgt gcaaaaaaaa tgaccaaaaa
     1261 ggcaaagaga agcaatttat atactcctaa agccgagatg ccaccggaac atctgcgaaa
     1321 gattattaat actcacagtg acatggcctc caaaatgtac aatactgata aaaaggcttt
     1381 tctaggcgct ctaaagtatt taccgcacgc gattttaaaa ttgttagaga acatgcctca
     1441 tccatgggag caagccaaag aagttaaagt tttatatcac acttcaggag ccataacatt
     1501 tgtgaatgaa acaccaagag taatagagcc tgtatatact gcccaatggt cagctacttg
     1561 gatagcaatg aggagagaaa aacgagatag aacacatttt aaaagaatga gatttccacc
     1621 tttcgatgat gacgaaccac ctctgtcata tgaacagcac atagaaaata tcgagccact
     1681 ggatcctata aatcttccct tagactctca agatgatgaa tatgttaaag attggctcta
     1741 cgattcccgg cccttggagg aggatagtaa aaaggtcaat ggtacatctt ataaaaagtg
     1801 gagttttgat ttacctgaaa tgtcgaactt atataggtta tcaacgccac ttagagatga
     1861 agtaactgac aaaaattact attatctttt cgataaaaaa tcttttttta acggtaaagc
     1921 ccttaataac gcaatccctg gaggtcctaa atttgaacct ttatacccaa gagaagagga
     1981 ggaagattat aacgaattca actctataga tagagtaata tttagggttc ctattagaag
     2041 cgaatataag gtagcgtttc ctcatctata taattcccgg ccccgttcag tgcgcatacc
     2101 atggtataat aatccagtgt cgtgtattat ccagaacgat gaggagtacg acacgcctgc
     2161 actttttttt gatccatcgc tgaacccaat acctcacttt attgataata attcatccct
     2221 caacgtatca aatacaaagg aaaacggtga tttcacttta cctgaagatt ttgcacctct
     2281 tttggctgaa gaagaagaac ttattttgcc aaatacaaaa gatgcgatgt cgttatatca
     2341 ttcacctttt ccatttaata gaactaaagg aaaaatggtg agagcacaag atgttgccct
     2401 ggctaaaaaa tggtttttgc aacatcctga cgaggagtat cccgttaaag ttaaggtctc
     2461 ttaccaaaag ctcttgaaga actacgtgct aaatgaattg caccctactt tgccaacaaa
     2521 ccacaacaaa actaagttat tgaagagttt aaaaaatacc aagtattttc aacaaactac
     2581 aattgattgg gtagaggcag gactccagct atgtcgacaa ggacacaata tgttaaattt
     2641 attaatccac agaaagggcc taacttattt acatctcgac tacaatttca atttaaaacc
     2701 aactaaaacc ttaaccacaa aagaacgtaa aaagtcaaga cttggaaact catttcattt
     2761 gatgcgtgaa ttgctaaaaa tgatgaaact gattgtagat acacatgtgc agtttaggtt
     2821 aggaaatgta gatgccttcc agttagcaga tggtatacat tatattttga accacatagg
     2881 acaattgacc ggtatttatc gttataaata taaagtaatg catcaaatcc gcgcatgtaa
     2941 agatctgaaa catatcatat attataagtt taataaaaat ttgggaaaag gacctggttg
     3001 tggattttgg caacctgcgt ggagagtgtg gctaaatttc ttaagaggca ctataccact
     3061 actcgaaaga tatattggaa accttatcac acgtcaattt gaaggccgtt ctaatgaaat
     3121 tgtcaaaact acaactaaac aaagacttga cgcctactac gatcttgagt tacgaaactc
     3181 ggtcatggat gacattttag aaatgatgcc tgagagtatt cgacagaaaa aggcaagaac
     3241 tatactgcaa catttaagtg aggcttggag atgttggaag gctaatattc catgggatgt
     3301 tcctggtatg ccagccccaa tcaaaaaaat aattgaacgg tatataaaat ccaaagctga
     3361 tgcctgggta tctgcagctc attacaatcg agaacgtata aagcgtggag ctcacgtcga
     3421 aaagactatg gttaaaaaaa atctggggcg tttaacgaga ctttggatca aaaatgagca
     3481 ggaacgtcaa aggcaaatac agaaaaacgg tcccgaaatt actcccgaag aggctacgac
     3541 catattttct gttatggttg agtggcttga atcaaggagc ttttcaccaa taccatttcc
     3601 acctttaact tacaaaaatg atactaagat attagttctt gccctcgaag acctaaaaga
     3661 tgtctacgca tcaaaagtac gtttaaatgc atctgaaagg gaagaacttg cgttgataga
     3721 ggaggcttat gataatcctc acgatacttt gaacagaatc aaaaagtact tgttgaccca
     3781 gcgagttttt aagcctgttg atataaccat gatggaaaac tatcaaaaca tttcccctgt
     3841 ttattcagtt gatcccttgg aaaagattac cgatgcatat cttgatcagt atttatggta
     3901 cgaagctgac caacgaaagc ttttcccaaa ctggattaaa ccaagtgatt cagagatacc
     3961 acctcttctg gtatacaagt ggactcaggg tataaacaac ctatctgaaa tttgggatgt
     4021 atccagaggc caatctgcag ttttgcttga gactactttg ggtgaaatgg ccgaaaaaat
     4081 tgactttact ttacttaata gattacttcg cctgattgta gatcctaata ttgctgacta
     4141 tatcactgca aaaaataatg ttgttatcaa ctttaaagat atgagtcacg tcaacaaata
     4201 tggcttaata cgcgggttga agttcgcttc tttcatattc caatattacg gactagttat
     4261 agatctttta ctattgggtc aggaaagggc tacagatttg gctggtccag ctaacaatcc
     4321 aaatgaattt atgcaattca agagcaaaga agtagaaaag gcacatccga tcagacttta
     4381 caccagatat ttagatcgta tatatatgct ttttcacttt gaagaagatg agggagagga
     4441 attgactgat gaatacttgg cagagaatcc agatccaaac tttgaaaaca gcatcggata
     4501 caataataga aaatgctggc ccaaagattc gcgcatgagg ctaatacgtc aggacgttaa
     4561 cctagggcga gcagtgtttt gggaaattca aagtagggtg ccaacttcgt taacgtcaat
     4621 taaatgggaa aatgcttttg tttccgtata cagtaaaaac aatccgaact tactcttctc
     4681 tatgtgtggt tttgaagtca gaatattacc aagacaaaga atggaagagg tggtttctaa
     4741 cgatgaaggt gtttgggatt tagttgacga aagaaccaag caaagaactg caaaagcata
     4801 tttaaaggta tctgaagaag aaatcaaaaa atttgacagc agaataagag gcatattgat
     4861 ggcctccggt tcaaccactt ttactaaagt ggccgctaaa tggaacactt cactgatatc
     4921 ccttttcaca tactttagag aagccattgt tgccacggag ccgttattag atatcctggt
     4981 gaaaggagaa acaagaattc aaaaccgtgt caaacttggt ttaaattcga aaatgccaac
     5041 aagatttcca cctgctgttt tttatacacc aaaagaactt ggtgggttgg gtatgattag
     5101 tgcttctcat attttaattc ctgcatctga cttaagttgg tcaaaacaga ccgatactgg
     5161 aattacgcat tttcgtgctg gtatgacgca cgaggatgaa aaattaatcc caactatctt
     5221 ccgttacatc actacttggg aaaatgaatt tttagactct caaagggtgt gggccgagta
     5281 tgcaacgaaa aggcaggaag ctattcagca gaataggagg ttagcttttg aagaacttga
     5341 aggatcgtgg gatcgtggta tacctcgtat tagcacttta tttcaaagag atcgacatac
     5401 tctagcgtat gacagaggtc acagaatccg tagggaattt aaacagtact ctctagagag
     5461 aaacagcccc ttttggtgga caaactccca ccatgacggt aaattatgga atttaaatgc
     5521 ctatagaact gatgtgattc aagccctagg aggtattgaa acaattttgg aacacacttt
     5581 atttaagggg actggtttta attcatggga aggtttgttt tgggaaaagg cgtccgggtt
     5641 tgaggattcc atgcaattta agaagctaac tcatgctcaa agaactggtt taagccaaat
     5701 tccaaatcgt agatttacct tatggtggtc accaactatt aacagagcaa atgtctatgt
     5761 cggtttcttg gtgcaattgg atctgactgg tatcttcctc catggtaaaa ttcctacgtt
     5821 aaaaatatct ttaattcaaa tattccgtgc tcatttgtgg caaaaaatac atgaaagtat
     5881 agtttttgat atttgtcaaa ttctcgatgg tgagctagat gttttgcaaa ttgaatctgt
     5941 gactaaagaa acagtgcatc cccgcaaatc gtacaagatg aattcatctg ccgcggacat
     6001 aactatggaa agtgttcatg aatgggaagt ttcaaaaccc tccctattac atgaaacaaa
     6061 tgactcattt aagggattga ttaccaacaa aatgtggttt gatgtacaat tgagatacgg
     6121 tgattatgat tctcatgata tatctcgtta tgtgagggca aaattcctcg attatacaac
     6181 agataatgtc agcatgtatc cctccccaac aggtgttatg atcggtatcg atttggctta
     6241 taacatgtat gatgcctatg gtaattggtt caacggatta aaacctttga tacaaaatag
     6301 tatgagaaca attatgaaag ctaacccagc attatatgtc cttcgtgaac gtattagaaa
     6361 aggtcttcaa atatatcaat ctagtgtaca ggaaccattt ttaaactcct caaactatgc
     6421 cgagttattc aataacgaca ttaaactctt cgttgacgac actaatgtgt atagagttac
     6481 tgtccacaag acttttgaag ggaacgttgc tacaaaagca attaatggtt gcattttcac
     6541 tttaaatcca aagactgggc atctgtttct gaaaattatc catacctctg tatgggccgg
     6601 tcaaaaacgt ttaagccaat tggccaaatg gaaaacagct gaggaagtta gtgctcttgt
     6661 gagatctttg cccaaagaag agcagccaaa acaaattatc gtcacaagaa aggcaatgct
     6721 tgatccttta gaggttcaca tgcttgactt tcctaacata gcaatcaggc caacagagct
     6781 gcgactacca ttttcagctg caatgtcaat agataaactt tctgatgttg tgatgaaagc
     6841 tactgaacct cagatggttc tgtttaacat ttatgatgat tggttagacc gtatttcatc
     6901 gtacactgca ttttctagac ttacactact tttaagagct ttaaagacga acgaggaaag
     6961 tgccaaaatg attctactca gtgaccccac gatcactatc aaatcgtacc atttatggcc
     7021 ttcattcact gatgaacaat ggatcactat cgaatctcaa atgagagatt tgattttgac
     7081 tgagtacggc agaaaataca atgtcaatat atctgcctta actcaaacgg aaattaaaga
     7141 tattatattg ggtcaaaata tcaaggcacc atctgtgaag agacaaaaaa tggctgaatt
     7201 agaagctgcc aggtcagaaa agcagaacga cgaagaagct gcaggtgcat caactgttat
     7261 gaaaactaaa actatcaatg cgcaaggtga ggagatagtt gtagtagctt ccgctgatta
     7321 cgaaagtcaa acgtttagtt caaagaatga atggagaaaa tctgcaattg ctaatacact
     7381 attatatttg aggttaaaaa acatttatgt ttcggctgat gatttcgtgg aggaacagaa
     7441 tgtttacgtc cttccaaaaa atttattaaa gaagtttatt gagatattcg atgtgaaaat
     7501 ccaggtggcg gcctttattt atggtatgtc agcaaaagat catccgaagg tgaaagaaat
     7561 caagactgtt gtgctggtac ctcaattagg ccacgtagga tccgttcaga taagcaacat
     7621 ccctgacatc ggagaccttc cggatactga aggccttgaa ctactcggtt ggatccatac
     7681 ccagacagaa gaacttaaat tcatggctgc gtctgaagta gcaactcatt ctaaactttt
     7741 tgcggataag aaaagagact gcattgatat ttcaattttt tccaccccgg gatcagtatc
     7801 attaagcgct tataacctga ccgacgaagg ttaccaatgg ggtgaagaaa ataaggatat
     7861 aatgaacgtg ctatcggaag ggtttgagcc tactttcagc acgcatgccc aattacttct
     7921 ctctgaccgt atcacaggta attttataat tccatctgga aacgtttgga actacacatt
     7981 tatgggaaca gcatttaacc aagaaggtga ctacaatttt aaatacggca ttcctttgga
     8041 attctataat gagatgcatc gtccagtaca ctttttacag tttagcgaat tggcggggga
     8101 cgaagagtta gaggccgaac aaatcgatgt atttagctga atatattccc caataaactg
     8161 gaatctgtta tttcatagat atatatgtaa gtataagttt gtatgtataa tattcaacgc
     8221 attttttatg cccgtcgtag ttgctaccta ccacacgatg atgtattata atttcggaac
     8281 tacttcaaag ctacgctttc cgcactatga aaagctataa gtatgtgaaa attggtcatt
     8341 gactgtgatt ctatttcaaa aagtaaccaa aggagataga catcgttcgg acagaaaatg
     8401 gtccgttgtc actcccattc cattatgtcc ttcatataga aattggatat tcttgttttc
     8461 atttcgcgca ataaagcaat tccgtgcggc agaaagaact tatatatatt taactgatct
     8521 tacgctattt atggcaaaac ttgtgttaca tttttgaaga taaagttaca atcattacgg
     8581 cagcctcaaa acaaaattgg gagaaaacat actcaagtga gtactcattt tgtgcaagca
     8641 aacactgaca attgaagaga tcgtcaggat gcccggaacg ccacagaaga acaagaggtc
     8701 tgcgagtata tctgtttcac ctgcgaagaa gacagaggaa aaagaaataa tacaaaatga
     8761 ttcaaaggcg atattatcta agcaaactaa gaggaaaaaa aagtatgctt ttgctcctat
     8821 aaataactta aatgggaaga acaccaaggt atccaatgca agtgtcctaa agtcaatagc
     8881 cgtttcacaa gtacgaaata catcaagaac aaaagatata aacaaagcag ttagcaagag
     8941 cgtaaagcaa ttaccaaatt cacaagttaa accgaaacgg gaaatgtcta atttgagtag
     9001 gcatcacgat ttcactcaag acgaggacgg ccccatggaa gaagtaatat ggaaatattc
     9061 acctttacaa cgagatatgt ccgacaaaac aacaagcgct gccgaatact ctgatgacta
     9121 cgaagatgtc caaaatccct cttctacacc tatagtccct aatcgactga aaactgttct
     9181 tagtttcaca aatatccaag tacccaacgc tgacgtaaat caactcattc aagaaaatgg
     9241 aaatgagcaa gtgcgtccta aaccagcaga aatatctacc agggagtctc tccgtaatat
     9301 agatgatatt ctcgatgata tagagggcga tttaactata aaaccgacga taacgaaatt
     9361 cagcgatttg ccatcttcac ccatcaaggc acccaacgtt gaaaaaaaag cagaggtgaa
     9421 tgcagaagaa gtagataaaa tggattcaac aggagatagc aacgatggcg atgactcttt
     9481 gatagatatt ttaactcaaa agtatgttga gaaacgcaag agtgagagtc agataacaat
     9541 tcaaggcaac accaatcaaa aaagtggagc ccaggaaagt tgtgggaaga atgataacac
     9601 aaaatcgaga ggagaaattg aagatcatga aaatgtagac aatcaagcca aaacaggcaa
     9661 tgcattttat gagaatgaag aagacagtaa ttgccaaaga attaaaaaaa atgaaaaaat
     9721 agaatacaat tcttcagacg aattttcaga cgattcacta attgaactat tgaacgaaac
     9781 tcaaacacaa gtggaaccta acactattga acaagattta gacaaagtgg agaaaatggt
     9841 ctctgacgat ttgagaattg ctactgattc cactttatcc gcatatgctc tcagagctaa
     9901 gtctggtgct ccaagagacg gcgtagtacg attagtaata gtaagcttac ctccaattcg
     9961 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    10021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10141 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10201 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10261 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10321 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10381 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10441 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10501 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10561 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
    10621 tacgcatctg tgcggtattt cacaccgcat agatccgtcg agttcaagag aaaaaaaaag
    10681 aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg
    10741 cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg
    10801 tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact
    10861 acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc
    10921 ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc
    10981 gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg
    11041 ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg
    11101 cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa
    11161 atcctgatcc aaaccttttt actccacgca cggcccctag ggcctcttta aaagcttgac
    11221 cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc
    11281 actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata tggtccagaa
    11341 accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct gctactgctt
    11401 ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc tttaaagaga
    11461 tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc tgctctgtca
    11521 tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa gaaatcacat
    11581 tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag
    11641 tcatccgcta ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga
    11701 gtgtactaga ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgtgttat
    11761 gacttccctg actaatgccg tgttcaaacg atacctggca gtgactccta gcgctcacca
    11821 agctcttaaa acgggaattt atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11881 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
    11941 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
    12001 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
    12061 gttaatgtca tgataataat ggtttcttag ggtccttttc atcacgtgct ataaaaataa
    12121 ttataattta aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa
    12181 ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac
    12241 aaatactacc ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc
    12301 ttgtctgtgt agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa
    12361 tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc
    12421 tttttgttga aattttttaa acctttgttt attttttttt cttcattccg taactcttct
    12481 accttcttta tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag
    12541 taaattccca aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc
    12601 gatccgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    12661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    12721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    12781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    12841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    12901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    12961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    13021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    13081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    13141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    13201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    13261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    13321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    13381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    13441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    13501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    13561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    13621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    13681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    13741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    13801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    13861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    13921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    13981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    14041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    14101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    14161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcgga
    14221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    14281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    14341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    14401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    14461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    14521 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    14581 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    14641 gcaat
//
