LOCUS       sequence-177962-        8692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red color variant of bright luminescent protein, fused to b-tubulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: tubulin-ReNL_pcDNA3
FEATURES             Location/Qualifiers
     source          1..8692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(433..1224,1225..1293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1056..1075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1294..1398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1473..1492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1664..2043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2044..2247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2198..2218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2292..2311
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2292..2310
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5674..5692)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5675..5692)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5712..5729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5718..5942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5988..6416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6075..6094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6285..6306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6425..6445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6430..6759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6610..6745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6672..6691
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6826..7620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6880..6899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7490..7509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7794..7915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7831..7850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7885..7904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7964..7980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7964..7980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7977..7999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7988..8004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8012..8018,8019..8036,8037..8042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8057..8078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8195..8212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8366..8692,1..262))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8446..8465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
       61 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
      961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     1441 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1501 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1561 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1621 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1681 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1741 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1801 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1861 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1921 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1981 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2041 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2101 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2161 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2221 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     2281 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttcgccacc atgagggaaa
     2341 tcgtgcacat ccaggccgga cagtgtggca accagatcgg tgctaagttc tgggaggtga
     2401 taagcgatga acatggcatc gaccccaccg gtacctacca cggtgacagc gacctgcagc
     2461 tggaccgaat ctctgtgtac tataatgaag ccacaggtgg caagtatgtc cctcgagcta
     2521 tcttggtgga tctagaacct gggactatgg actccgttcg ctcaggtcct tttggccaga
     2581 tcttcagacc agacaacttc gttttcggtc agtctggggc aggcaacaac tgggctaaag
     2641 gccactacac agagggagct gagttggttg actctgtctt ggatgtggtg cggaaggagg
     2701 cggagagctg tgattgcctg caaggctttc agctgaccca ctcactgggt ggaggcactg
     2761 gctctggcat gggcaccctg ctcatcagca agatccggga agaatatcct gaccgtatca
     2821 tgaatacctt cagtgtggtg ccctcgccca aagtgtctga taccgtggtc gagccctaca
     2881 atgccaccct gtctgtccat cagttggttg agaacacgga tgagacctac tgcatcgaca
     2941 acgaggccct ctacgacatc tgcttccgta ccctcaagct caccacgcca acctacggag
     3001 acctgaacca tctcgtctcg gccaccatga gcggcgtcac cacctgcctc cgtttcccgg
     3061 gccagcttaa tgctgacctt cgaaagctgg ctgtcaacat ggtgccattc ccacgtctcc
     3121 acttcttcat gcctggcttt gcccctctca ccagccgtgg aagccagcag taccgggccc
     3181 tcactgtgcc tgaacttacc cagcaggtct tcgatgccaa gaacatgatg gccgcctgcg
     3241 acccgcgcca cggccggtac ctcacagttg ccgccgtctt ccgtggacgg atgtccatga
     3301 aggaggtgga tgagcagatg ctcaacgtgc agaacaagaa tagcagctac ttcgtggaat
     3361 ggatccccaa caatgtcaag acagctgtct gtgacatccc accgcgtggc ctcaagatgg
     3421 cagtcacctt cattggaaac agcacagcca tccaggagct cttcaagcgc atctctgagc
     3481 agtttacggc tatgttccgc cggaaggctt tcctccactg gtatacgggt gagggcatgg
     3541 acgagatgga gttcaccgaa gctgagagca acatgaacga cctcgtctct gagtaccagc
     3601 agtaccagga tgccaccgcg gaagaggaag aggatttcgg agaggaggca gaagaggagg
     3661 cccagtcgac ggtaccgcgg gcccgggatc caggatctgg cggaggaagt gggggaggat
     3721 ctggagaatt catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
     3781 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     3841 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     3901 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     3961 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     4021 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     4081 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     4141 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     4201 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     4261 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     4321 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     4381 gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca
     4441 gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag ttcatgcgct
     4501 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     4561 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     4621 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
     4681 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
     4741 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
     4801 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
     4861 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     4921 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
     4981 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
     5041 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5101 acgagcgctc cgagggccgc caccacctgt tccggctgga agatttcgtt ggggactggc
     5161 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     5221 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     5281 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     5341 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     5401 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     5461 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     5521 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     5581 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag
     5641 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     5701 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5761 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5821 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5881 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5941 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6661 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6781 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6841 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6901 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6961 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7021 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7081 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7141 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7201 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7261 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7321 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     7381 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7441 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7501 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7561 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7621 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7681 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7741 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7801 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7861 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7921 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7981 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8041 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8101 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8161 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8221 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8281 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta ag
//
