LOCUS       sequence-177963-        8589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red color variant of bright luminescent protein, fused to 
            Lysosomal-associated membrane protein 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: LAMP-ReNL_pcDNA3
FEATURES             Location/Qualifiers
     source          1..8589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        184..563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        564..767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     718..738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     812..831
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        812..830
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      845..854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      849..858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(851..913,914..2071)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     promoter        complement(4091..4109)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4092..4109)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4129..4146)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4135..4359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4405..4833
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4492..4511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4702..4723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4842..4862)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4847..5176
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5027..5162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5089..5108
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5243..6037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5297..5316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5907..5926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6211..6332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6248..6267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6302..6321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6381..6397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6381..6397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6394..6416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6405..6421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6429..6435,6436..6453,6454..6459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6474..6495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6612..6629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6783..7371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6863..6882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7542..8333,8334..8402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8165..8184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8403..8507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(8582..8589,1..12))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
       61 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
      121 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      181 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      241 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      301 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      361 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      421 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      481 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      541 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      601 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      661 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      721 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
      781 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
      841 cttcgccacc atggcggccc cgggcgcccg gcgtccgctg ctcctgttgc tgctggcagg
      901 ccttgcacac agcgccccag cactgttcga ggtgaaagac aacaacggta cagcgtgtat
      961 aatggccagc ttctctgcct cctttctgac cacctatgag gctggacatg tttctaaggt
     1021 ctcgaatatg accctgccag cctctgcaga agtcctgaag aatagcagct cttgtggtga
     1081 aaagaatgct tctgagccca ccctcgcaat cacctttgga gaaggatatt tactgaaact
     1141 caccttcaca aaaaacacaa cacgttacag tgtccagcac atgtatttca catataacct
     1201 gtcagacaca caattctttc ccaatgccag ctccaaaggg cccgacactg tggattccac
     1261 aactgacatc aaggcagaca tcaacaaaac ataccgatgt gtcagcgaca tcagggtcta
     1321 catgaagaat gtgaccattg tgctctggga cgctactatc caggcctacc tgccgagtag
     1381 caacttcagc aaggaagaga cacgctgccc acaggatcaa ccttccccaa ctactgggcc
     1441 acccagcccc tcaccaccac ttgtgcccac aaaccccagt gtgtccaagt acaatgtgac
     1501 tggtgacaat ggaacctgcc tgctggcctc tatggcactg caactcaaca tcacctacat
     1561 gaagaaggac aacacgactg tgaccagagc attcaacatc aacccaagtg acaaatatag
     1621 tgggacttgc ggtgcccagt tggtgaccct gaaggtgggg aacaagagca gagtcctgga
     1681 gctgcagttt gggatgaatg ccacttctag cctgtttttc ctgcaaggag ttcagttgaa
     1741 catgactctt cctgatgcca tagagcccac gttcagcacc tccaactatt ccctgaaagc
     1801 tcttcaggcc agtgtcggca actcatacaa gtgcaacagt gaggagcaca tctttgtcag
     1861 caaggcgctc gccctcaatg tcttcagcgt gcaagtccag gctttcaggg tagaaagtga
     1921 caggtttggg tctgtggaag agtgtgtaca ggacggtaac aacatgctga tccccattgc
     1981 tgtgggcggg gccctggcag ggctggtcct catcgtcctc atcgcctacc tcatcggcag
     2041 gaagaggagt cacgcgggct atcagaccat cggtaccggc agcggtggcg gtggcagcgg
     2101 tggcggtggc agcggtggat ccatggtgag caagggcgag gaggtcatca aagagttcat
     2161 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     2221 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     2281 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     2341 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     2401 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     2461 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     2521 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     2581 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     2641 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     2701 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2761 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     2821 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     2881 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     2941 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3001 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     3061 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     3121 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     3181 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     3241 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     3301 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     3361 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     3421 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     3481 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttccggctgg aagatttcgt
     3541 tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt
     3601 gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag
     3661 cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg
     3721 cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca
     3781 ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat
     3841 cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt
     3901 aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg
     3961 ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat
     4021 tctggcgtaa gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag
     4081 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     4141 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4201 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4261 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4321 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4381 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4441 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4501 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4561 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4621 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4681 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4741 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4801 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4921 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4981 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5041 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5101 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5161 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5221 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5281 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5341 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5401 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5461 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5521 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5581 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5641 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5701 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5761 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5821 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5881 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5941 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6001 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6061 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6121 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6181 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6361 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8521 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8581 gtacaatct
//
