LOCUS       sequence-177964-        4768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red color variant of bright luminescent protein for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: ReNL_pRSETb
FEATURES             Location/Qualifiers
     source          1..4768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      125..713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     614..633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     867..884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     969..988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        969..987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1019..1041
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1061..1078
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1082..1114
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1118..1126,1127..1141)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(3118..3136)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3132..3179
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3276..3731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3363..3382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3573..3594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3757..3861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3862..3930,3931..4722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4080..4099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg atctcgatcc
      961 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gaaataattt
     1021 tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg
     1081 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
     1141 ggatccgatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     1201 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1261 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     1321 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     1381 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1441 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     1501 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     1561 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     1621 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     1681 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     1741 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     1801 cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc
     1861 cggcaccgcc tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa
     1921 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1981 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     2041 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     2101 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     2161 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     2221 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     2281 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     2341 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     2401 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     2461 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2521 gcgctccgag ggccgccacc acctgttccg gctggaagat ttcgttgggg actggcgaca
     2581 gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca
     2641 gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct
     2701 gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc aaatgggcca
     2761 gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta aggtgatcct
     2821 gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggacg
     2881 gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag ggaccctgtg
     2941 gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg
     3001 agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg cgtaagaatt
     3061 cgaagcttga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     3121 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     3181 tgaaaggagg aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc
     3241 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3301 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3361 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3421 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3481 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3541 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3601 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3661 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3721 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3841 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4741 tcatatatac tttagattga tttaaaac
//
