LOCUS       sequence-177988-       10718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB 
            (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control 
            of BAD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Muller J, MacEachran D, Burd H, Sathitsuksanoh N, Bi C, Yeh YC, Lee 
            TS, Hillson NJ, Chhabra SR, Singer SW, Beller HR
  TITLE     Engineering of Ralstonia eutropha H16 for autotrophic and 
            heterotrophic production of methyl ketones.
  JOURNAL   Appl Environ Microbiol. 2013 Jul;79(14):4433-9. doi: 
            10.1128/AEM.00973-13. Epub 2013 May 17.
  PUBMED    23686271
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00973-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-07"; volume: "79"; issue: "14"; pages: "4433-9"
COMMENT     Sequence Label: JM3 [JBEI-6891]
FEATURES             Location/Qualifiers
     source          1..10718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      574..645
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      661..688
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      1889..2660
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2661..3323
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(3466..3560)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3591..4385)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3702..3721)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4312..4331
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(4528..5406)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRDQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5433..5717
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5640..5659
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ccgcgagaag gcggcgcgag gccgccgcgg ctgaccgcgg ccgtccccgg acgcaccggg
       61 cccccgtcac ctcgcggtgg ggatccgagc tccaattcgc cctatagtga caacgtaagg
      121 ttattgcgct atgcaaacac aaatcaaagt tcgtggatat catctcgacg tttaccagca
      181 cgtcaacaac gcccgctacc ttgaatttct cgaagaagcc cgctgggatg ggttggaaaa
      241 tagcgacagt tttcagtgga tgacggccca taacatcgcc ttcgtcgtgg tcaatatcaa
      301 tattaactat cgtcgcccag cggtattaag tgacctgtta actattacca gtcagttgca
      361 gcaattaaac ggtaaaagcg gcatcttaag ccaggtcatt acactggagc cggaagggca
      421 ggtggtagcg gatgcgctta ttacgtttgt ttgtattgat cttaaaacgc agaaagcatt
      481 agctctggaa ggggaattgc gcgaaaagct ggagcagatg gttaagtaac tcgaggctag
      541 cggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa
      601 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      661 ggctcacctt cgggtgggcc tttctgcgtt tatacctagg gcgttcggct gcggctggcg
      721 ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc agcagctttt cgcccacggc
      781 cttgatgatc gcggcggcct tggcctgcat atcccgattc aacggcccca gggcgtccag
      841 aacgggcttc aggcgctccc gaaggtctcg ggccgtctct tgggcttgat cggccttctt
      901 gcgcatctca cgcgctcctg cggcggcctg tagggcaggc tcatacccct gccgaaccgc
      961 ttttgtcagc cggtcggcca cggcttccgg cgtctcaacg cgctttgaga ttcccagctt
     1021 ttcggccaat ccctgcggtg cataggcgcg tggctcgacc gcttgcgggc tgatggtgac
     1081 gtggcccact ggtggccgct ccagggcctc gtagaacgcc tgaatgcgcg tgtgacgtgc
     1141 cttgctgccc tcgatgcccc gttgcagccc tagatcggcc acagcggccg caaacgtggt
     1201 ctggtcgcgg gtcatctgcg ctttgttgcc gatgaactcc ttggccgaca gcctgccgtc
     1261 ctgcgtcagc ggcaccacga acgcggtcat gtgcgggctg gtttcgtcac ggtggatgct
     1321 ggccgtcacg atgcgatccg ccccgtactt gtccgccagc cacttgtgcg ccttctcgaa
     1381 gaacgccgcc tgctgttctt ggctggccga cttccaccat tccgggctgg ccgtcatgac
     1441 gtactcgacc gccaacacag cgtccttgcg ccgcttctct ggcagcaact cgcgcagtcg
     1501 gcccatcgtt tcatcggtgc tgctggccgc ccagtgctcg ttctctggcg tcctgctggc
     1561 gtcagcgttg ggcgtctcgc gctcgcggta ggcgtgcttg agactggccg ccacgttgcc
     1621 cattttcgcc agcttcttgc atcgcatgat cgcgtatgcc gccatgcctg cccctccctt
     1681 ttggtgtcca accggctcga cgggggcagc gcaaggcggt gcctccggcg ggccactcaa
     1741 tgcttgagta tactcactag actttgcttc gcaaagtcgt gaccgcctac ggcggctgcg
     1801 gcgccctacg ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct cccttgccag
     1861 cccgtggata tgtggacgat ggccgcgagc ggccaccggc tggctcgctt cgctcggccc
     1921 gtggacaacc ctgctggaca agctgatgga caggctgcgc ctgcccacga gcttgaccac
     1981 agggattgcc caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg
     2041 cctgcggcct tgccccatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg
     2101 gcctgcgcgc ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag
     2161 cgaccgcgca gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg
     2221 gcagtcgaag ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg
     2281 gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc
     2341 cccggagggg ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc
     2401 aggggtgccc ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa
     2461 aaatcaacaa cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag
     2521 ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt
     2581 gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc
     2641 cctaccgcat ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga
     2701 acaagcccgg tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta
     2761 ttgcgaggaa acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc
     2821 agaacgccgt ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga
     2881 cggtccaata cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca
     2941 acggccccgg caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc
     3001 gcgaccagtt gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg
     3061 acgaatcgct gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc
     3121 agcaactacc gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg
     3181 aaccagacct gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc
     3241 tgccgatgcc cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac
     3301 gggtcacgct gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc
     3361 cagactatcg gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     3421 cgcggtgcat ggagccgggc cacctcgacc tgaatggaag cttggattct caccaataaa
     3481 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     3541 tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac
     3601 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     3661 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     3721 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     3781 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     3841 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     3901 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     3961 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4021 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4081 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4141 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4201 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4261 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4321 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     4381 atcatgcgaa acgatcctca tcctgtctct tgatcagatc atgatcccct gcgccatcag
     4441 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     4501 ggcgccccag ctggcaattc cgacgtctta tgacaacttg acggctacat cattcacttt
     4561 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
     4621 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
     4681 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
     4741 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
     4801 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
     4861 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
     4921 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
     4981 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
     5041 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
     5101 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     5161 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     5221 tcgtccctga tttttcacca ccccctgatc gcgaatggtg agattgagaa tataaccttt
     5281 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     5341 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
     5401 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     5461 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     5521 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     5581 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     5641 tgccatagca tttttatcca taagattagc ggattctacc tgacgctttt tatcgcaact
     5701 ctctactgtt tctccatacc cgtttttttg ggaattcaaa ggaggccatc ctatggcgga
     5761 cacgttattg attctgggtg atagcctgag cgccgggtat cgaatgtctg ccagcgcggc
     5821 ctggcctgcc ttgttgaatg ataagtggca gagtaaaacg tcggtagtta atgccagcat
     5881 cagcggcgac acctcgcaac aaggactggc gcgccttccg gctctgctga aacagcatca
     5941 gccgcgttgg gtgctggttg aactgggcgg caatgacggt ttgcgtggtt ttcagccaca
     6001 gcaaaccgag caaacgctgc gccagatttt gcaggatgtc aaagccgcca acgctgaacc
     6061 attgttaatg caaatacgtc tgcctgcaaa ctatggtcgc cgttataatg aagcctttag
     6121 cgccatttac cccaaactcg ccaaagagtt tgatgttccg ctgctgccct tttttatgga
     6181 agaggtctac ctcaagccac aatggatgca ggatgacggt attcatccca accgcgacgc
     6241 ccagccgttt attgccgact ggatggcgaa gcagttgcag cctttagtaa atcatgactc
     6301 ataagagctc ggtacgacca gatcaccttg cggattcagg agactgacat gctttacaaa
     6361 ggcgacaccc tgtaccttga ctggctggaa gatggcattg ccgaactggt atttgatgcc
     6421 ccaggttcag ttaataaact cgacactgcg accgtcgcca gcctcggcga ggccatcggc
     6481 gtgctggaac agcaatcaga tctaaaaggg ctgctgctgc gttcgaacaa agcagccttt
     6541 atcgtcggtg ctgatatcac cgaatttttg tccctgttcc tcgttcctga agaacagtta
     6601 agtcagtggc tgcactttgc caatagcgtg tttaatcgcc tggaagatct gccggtgccg
     6661 accattgctg ccgtcaatgg ctatgcgctg ggcggtggct gcgaatgcgt gctggcgacc
     6721 gattatcgtc tggcgacgcc ggatctgcgc atcggtctgc cggaaaccaa actgggcatc
     6781 atgcctggct ttggcggttc tgtacgtatg ccacgtatgc tgggcgctga cagtgcgctg
     6841 gaaatcattg ccgccggtaa agatgtcggc gcggatcagg cgctgaaaat cggtctggtg
     6901 gatggcgtag tcaaagcaga aaaactggtt gaaggcgcaa aggcggtttt acgccaggcc
     6961 attaacggcg acctcgactg gaaagcaaaa cgtcagccga agctggaacc actaaaactg
     7021 agcaagattg aagccaccat gagcttcacc atcgctaaag ggatggtcgc acaaacagcg
     7081 gggaaacatt atccggcccc catcaccgca gtaaaaacca ttgaagctgc ggcccgtttt
     7141 ggtcgtgaag aagccttaaa cctggaaaac aaaagttttg tcccgctggc gcataccaac
     7201 gaagcccgcg cactggtcgg cattttcctt aacgatcaat atgtaaaagg caaagcgaag
     7261 aaactcacca aagacgttga aaccccgaaa caggccgcgg tgctgggtgc aggcattatg
     7321 ggcggcggca tcgcttacca gtctgcgtgg aaaggcgtgc cggttgtcat gaaagatatc
     7381 aacgacaagt cgttaaccct cggcatgacc gaagccgcga aactgctgaa caagcagctt
     7441 gagcgcggca agatcgatgg tctgaaactg gctggcgtga tctccacaat ccacccaacg
     7501 ctcgactacg ccggatttga ccgcgtggat attgtggtag aagcggttgt tgaaaacccg
     7561 aaagtgaaaa aagccgtact ggcagaaacc gaacaaaaag tacgccagga taccgtgctg
     7621 gcgtctaaca cttcaaccat tcctatcagc gaactggcca acgcgctgga acgcccggaa
     7681 aacttctgcg ggatgcactt ctttaacccg gtccaccgaa tgccgttggt agaaattatt
     7741 cgcggcgaga aaagctccga cgaaaccatc gcgaaagttg tcgcctgggc gagcaagatg
     7801 ggcaagacgc cgattgtggt taacgactgc cccggcttct ttgttaaccg cgtgctgttc
     7861 ccgtatttcg ccggtttcag ccagctgctg cgcgacggcg cggatttccg caagatcgac
     7921 aaagtgatgg aaaaacagtt tggctggccg atgggcccgg catatctgct ggacgttgtg
     7981 ggcattgata ccgcgcatca cgctcaggct gtcatggcag caggcttccc gcagcggatg
     8041 cagaaagatt accgcgatgc catcgacgcg ctgtttgatg ccaaccgctt tggtcagaag
     8101 aacggcctcg gtttctggcg ttataaagaa gacagcaaag gtaagccgaa gaaagaagaa
     8161 gacgccgccg ttgaagacct gctggcagaa gtgagccagc cgaagcgcga tttcagcgaa
     8221 gaagagatta tcgcccgcat gatgatcccg atggtcaacg aagtggtgcg ctgtctggag
     8281 gaaggcatta tcgccactcc ggcggaagcg gatatggcgc tggtctacgg cctgggcttc
     8341 cctccgttcc acggcggcgc gttccgctgg ctggacaccc tcggtagcgc aaaatacctc
     8401 gatatggcac agcaatatca gcacctcggc ccgctgtatg aagtgccgga aggtctgcgt
     8461 aataaagcgc gtcataacga accgtactat cctccggttg agccagcccg tccggttggc
     8521 gacctgaaaa cggcttaagg agtcacaatg gaacaggttg tcattgtcga tgcaattcgc
     8581 accccgatgg gccgttcgaa ggatccactc acgaacagga gcatcgtcat gaccgtccac
     8641 gagaagctcg ccccccagtc cccgacgcac agcaccgagg tccccaccga cgtcgccgag
     8701 atcgcgcccg agcggccgac gcccggctcc ctcgacgccg cggccctcga ggaggccctc
     8761 ctcggccgct gggccgcgga gcgccgcgaa tcccgcgaac tcgccaagga ccccgccctg
     8821 tggcgcgacc cgctgctcgg catggacgag caccgagccc gcgtcctgcg gcagctgggc
     8881 gtcctcgtgg agcgcaacgc ggtccaccgc gccttcccgc gcgagttcgg cggcgaggac
     8941 aaccacggcg gcaacatctc cgccttcggc gacctcgtgc tcgccgaccc ctccctgcag
     9001 atcaaggccg gtgtgcagtg gggcctgttc agctcggcga tcctgcacct gggcaccgcc
     9061 gagcaccacc gccggtggct gccgggcgcg atggacctga gcgtcccggg cgccttcgcc
     9121 atgaccgaga tcgggcacgg ctcggacgtc gcctcgatcg ccacgaccgc cacctatgac
     9181 gaggcgaccc aggagttcgt catccacacc ccgttcaagg gcgcctggaa ggactacctc
     9241 ggcaacgccg cgctgcacgg cagggcggcc acggtcttcg cgcagctgat cacccagggc
     9301 gtcaaccacg gcgtgcactg cttctacgtc cccatccggg acgagaaggg cgccttcctg
     9361 cccggcgtgg gcggcgagga cgacggcctc aagggcggac tgaacggcat cgacaacggc
     9421 cgcctgcact tcacccaggt ccgcatcccg cgcaccaacc tcctcaaccg ctacggcgac
     9481 gtggcggagg acgggaccta ctcctcgccc atcgcctccc ccgggcgtcg cttcttcacg
     9541 atgctcggca ccctcgtcca gggccgcgtc tccctgtccc tcgccgccac cacggcctcc
     9601 ttcctcggcc tgcacggtgc gctggcctac gcggagcagc gccgccagtt caacgcctcc
     9661 gacccgcagc gggaggaggt gctgctggac taccagaacc accagcgccg cctcatcgac
     9721 cgcctggccc gcgcctacgc ggacgccttc gcctccaacg agctcgtcgt gaagttcgac
     9781 gacgtcttct ccggccgctc cgacacggac gtggaccggc aggagctcga gacgctcgcc
     9841 gcggccgtga agccgctgac cacgtggcat gccctggaca ccctgcagga ggcccgcgag
     9901 gcgtgcggcg gcgccgggtt cctcgccgag aaccgggtga cccagatgcg cgccgacctg
     9961 gacgtgtacg tcacgttcga gggcgacaac acggtgctcc tgcagctggt gggcaagcgt
    10021 ctgctgaccg actactcgaa ggagttcgga cgtctcaatg tgggcgccgt ctcccgctac
    10081 gtggtccatc aggcctcgga cgccatccac cgtgcgggcc tgcacaaggc cgtccagtct
    10141 gtggccgacg gcggctccga gcgccgctcc gcgaactggt tcaaggaccc cgccgtgcag
    10201 catgagctgc tcaccgagcg cgtccgcgcc aagaccgccg acgtcgccgg aaccctctcg
    10261 ggggcccggg gcaagggcca ggcggctcag gcggaggcgt tcaacacgcg ccagcacgag
    10321 ctcatcgagg ccgcccgcaa ccacggcgag ctgctgcagt gggaggcctt cacccgcgcc
    10381 ctggagggca tcacggatga gaccacgaag actgtcctga cgtggctgcg cgacctcttc
    10441 gcgctgcgcc tcatcgagga cgacctcggc tggttcgtgg cccacggccg cgtctcgagc
    10501 cagcgcgccc gggccctgcg cggctatgtc aaccggctcg ccgagcggct gcgccccttc
    10561 gccctcgagc tcgtcgaggc gttcggcctc gagcccgagc acctgcgcat ggcggtggcc
    10621 acggacgccg agacccagcg ccaggaggag gcccacgcct ggttcacggc ccgccgggcc
    10681 gccggcgagg agccggagga cgagaaggcc gtgcgcgc
//
