LOCUS       sequence-178000-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sMTase system for targeted DNA methylation in mammalian 
            cells. Contains an eGFP marker expressed off separate promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Xiong T, Meister GE, Workman RE, Kato NC, Spellberg MJ, Turker F, 
            Timp W, Ostermeier M, Novina CD
  TITLE     Targeted DNA methylation in human cells using engineered 
            dCas9-methyltransferases.
  JOURNAL   Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.1038/s41598-017-06757-0.
  PUBMED    28751638
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-07-27"; volume: "7(1):6732. doi"; pages: " 
            10.1038/s41598-017-06757-0"
COMMENT     Sequence Label: pCMV sMTase (eGFP)
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..71
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     56..72
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(114..147)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        176..555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        556..759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     710..730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             837..884
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             888..911
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5475..5495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5535..6103
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5553..6105
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5697..5714)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5924..5943
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6120..6140
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6141..6167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        7117..7523
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7577..7586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7583..7585,7586..7588,7589..8302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7583..7585,7586..7588,7589..8299)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7628..7649)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7889..7908)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8236..8257
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8436..8517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8473..8492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8567..8671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8672..8740,8741..9532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8890..8909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9703..10291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10192..10211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttacggttcc tggccttttg ctggcctttt gctcacatgt ctggtgaatt caagtcgagg
       61 tcgacggtat cgataagctc gcttcacgag attccagcag gtcgagggac ctaataactt
      121 cgtatagcat acattatacg aagttatatt aagggttcca agcttaagtc gcgttgacat
      181 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      541 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      601 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      661 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      721 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      781 actgcttact ggcatcccag tgtggtggta cgggaattga cgtcggcatg ccagtgaaga
      841 gacctgctgc caccaagaaa gctggccagg ccaaaaagaa gaaactggac tacaaagacg
      901 atgacgacaa gacgcgtgat aagaaataca gcataggact ggccattgga acaaattctg
      961 tcggctgggc ggttattacc gatgaatata aggttccgtc caagaaattc aaggttctag
     1021 gcaatacaga ccgccacagt attaaaaaga atttgatagg tgcccttctg tttgacagtg
     1081 gcgagacagc ggaagcgacc agactcaaac gaacagccag gagacgttat acaagacgaa
     1141 agaataggat ctgttatctt caagaaatct ttagcaatga gatggcgaaa gtggatgata
     1201 gtttctttca tcgccttgaa gagtcctttc tggtagaaga agacaagaag catgaaagac
     1261 atcccatctt tggcaatata gtggatgaag ttgcctatca tgagaaatat ccaaccatct
     1321 accatctacg caagaaactg gtggattcca ccgataaagc ggatctgcgc ctgatctatc
     1381 tagcactagc gcacatgatc aagtttagag ggcattttct gatcgagggc gatctgaatc
     1441 ccgataatag tgatgtagac aaacttttca ttcaactggt gcagacttac aatcagctgt
     1501 ttgaagaaaa ccccatcaac gctagtggcg tggatgccaa agcgatcctt tccgctcgcc
     1561 tgagtaaaag cagacgcctg gaaaatctca tcgcccaact cccgggggag aagaaaaatg
     1621 gactgtttgg taacctcatc gccctgagcc tggggctgac tcccaatttt aaaagcaatt
     1681 ttgatctggc tgaagatgcc aaactgcaac ttagcaaaga tacctacgat gatgatctgg
     1741 ataatctgct ggcgcagatc ggcgatcagt atgccgatct gtttctggct gccaagaatc
     1801 tgagcgatgc catcctgctt agcgatattt tgagagtgaa taccgaaata accaaggccc
     1861 cgttgagcgc cagcatgatc aaacgctacg atgaacatca tcaggacctg acccttctga
     1921 aagccctggt tcgccagcag cttcctgaaa agtataaaga gattttcttt gatcagagca
     1981 agaacggcta tgctgggtat atcgatggtg gcgcctctca ggaagagttc tataagttca
     2041 ttaaaccaat cctggaaaag atggatggga ccgaggaact gctggtaaaa ttgaataggg
     2101 aggatctgct acgcaagcag cgaacttttg acaacgggtc catcccgcat cagatccacc
     2161 tgggggagct acatgccatc ctgagaagac aggaagactt ttatccattt ctgaaagaca
     2221 atcgggagaa gatcgaaaag attctgacct ttcgcatccc ctattatgtt gggcctctgg
     2281 cgagaggaaa cagtaggttt gcttggatga cccgaaagtc cgaagaaaca atcactcctt
     2341 ggaacttcga agaagttgtc gataaagggg ccagcgccca gagctttatc gaacgcatga
     2401 caaactttga taagaatctt ccaaatgaaa aggtgcttcc taaacatagt ctgctttatg
     2461 agtattttac ggtttataac gaactgacaa aggtcaaata tgttaccgaa ggaatgcgca
     2521 aaccagcttt tcttagcggg gaacaaaaga aagcaatcgt tgatctgctc ttcaaaacaa
     2581 atcgcaaagt gactgttaag cagctgaaag aagattattt caagaagata gaatgttttg
     2641 atagtgttga aatcagcggc gttgaagata gattcaatgc cagcctgggg acttaccatg
     2701 atctgttgaa aatcatcaag gataaagact tcctggataa cgaagaaaat gaggatattc
     2761 tggaagatat cgttctgaca ctgactctgt ttgaagatcg tgagatgatc gaggaaagac
     2821 ttaaaactta tgcccacctc tttgatgata aggtaatgaa acaacttaaa aggcgcagat
     2881 ataccgggtg gggcagactg tcccgcaaac tgatcaatgg aatccgtgat aagcagtccg
     2941 gaaagacaat actggatttt ctgaaaagcg atgggtttgc aaatcgcaat tttatgcaac
     3001 taattcatga tgatagtctg acattcaaag aagacatcca gaaagctcag gtatccggcc
     3061 agggagatag tctgcatgaa catatcgcta atctggccgg gtctcccgcc atcaagaaag
     3121 gaatcctgca aaccgtgaaa gttgttgatg aactggtcaa agtgatgggt cgacataagc
     3181 cagaaaatat tgttatcgaa atggctagag aaaaccaaac aacccagaag ggacaaaaga
     3241 actcgcgcga gaggatgaaa cgcattgaag aagggattaa agaactgggc agtcaaatcc
     3301 ttaaagagca tcccgttgaa aatacccaac tgcaaaacga aaagctctat ctctattatc
     3361 tccagaatgg cagagacatg tatgtagacc aggaactgga tatcaatagg ctgagtgatt
     3421 atgatgtcgc cgctatcgtt ccacagagtt tccttaaaga cgatagcata gacaataagg
     3481 tcctgacgag atccgataag aatagaggga aatcggataa cgttcctagt gaagaagtgg
     3541 tcaagaagat gaagaactat tggagacagc ttttgaacgc aaagctgatt acccagagga
     3601 agttcgataa tctgacgaaa gccgaaagag gcgggctgag tgaacttgat aaagccgggt
     3661 ttattaaacg ccagctggtt gaaacccgcc agattaccaa gcatgtagct cagatcctgg
     3721 atagtcgcat gaataccaaa tacgatgaaa atgataaact tatccgcgag gttaaagtaa
     3781 tcactctgaa atccaaactg gtttccgact tccgcaaaga tttccagttc tataaagtga
     3841 gagagatcaa caattaccat catgcacatg atgcgtattt gaatgcagtc gttggcaccg
     3901 ccctgatcaa gaagtatcca aaacttgaat cggagtttgt ctatggggat tataaagttt
     3961 atgatgttag gaaaatgatc gccaagtccg agcaggaaat aggaaaagct actgctaagt
     4021 atttctttta ctccaatatt atgaacttct tcaaaacaga aatcacactt gctaatggcg
     4081 agatccgcaa acgccccttg attgaaacca atggtgaaac cggcgaaatc gtctgggata
     4141 aaggtcgcga ttttgcaaca gtacgcaaag tgctgtcaat gccgcaggtc aatatcgtca
     4201 agaaaacaga agtgcaaaca ggaggcttct caaaggagag catcctgcct aaacggaact
     4261 cggacaagct tatcgccagg aagaaagact gggacccaaa gaaatatgga gggtttgata
     4321 gtcctacggt ggcctatagc gtccttgtag ttgccaaggt agagaagggt aagtcgaaga
     4381 agctgaaatc agttaaagag ctgcttggta ttacaatcat ggaaagaagt tcatttgaaa
     4441 agaatccgat cgactttctg gaagccaaag gctataagga agttaagaaa gacctgatta
     4501 tcaaacttcc caaatatagt cttttcgagc tggaaaacgg gaggaaacga atgctagcca
     4561 gtgcaggcga actgcagaaa ggcaatgagc tagccctacc atctaagtat gtaaacttcc
     4621 tgtatctggc cagtcattat gaaaagctga aggggagtcc agaagataac gaacagaagc
     4681 agctgtttgt agagcaacat aagcattatc tggatgagat catcgagcag attagtgaat
     4741 tttccaagag ggttatcctg gctgatgcaa atctggataa agttcttagt gcttataaca
     4801 aacatagaga caaaccaata agggaacagg ctgaaaatat catccatctg tttacgctga
     4861 cgaatcttgg cgccccagcc gccttcaagt atttcgatac aacaatcgat aggaaacgct
     4921 atacgtccac aaaagaagtt ctggatgcaa cccttattca tcagtcaatt accgggcttt
     4981 atgaaacacg catcgatctg agtcaacttg gaggggaccc tgcttctggc ggaacttcat
     5041 ctgatggtgg cacgtcagac ggagggtcaa gcaacacagg cggtagctct gacggaggga
     5101 gctcagaagg cgaacctgcg catgcactga agtacaacct gactgagttc aagaagacca
     5161 aatcaaacat caacaaggca agcctcattg ggtatagcaa gttcaactca gaaggctacg
     5221 tgtacgaccc tgagttcaca ggaccaaccc tgactgcaag tggagcaaac tcacggatca
     5281 agatcaagga cggatctaac attcgcaaga tgaactcaga tgaaacgttt ctgtacatgg
     5341 gcttcgattc acaagatgga aagagggtga acgagattga gttcctgact gaaaaccaga
     5401 agatcttcgt gtgcggaaac tcaatctccg tggaagttct tgaagcgatc atcgacaaga
     5461 ttggagggac cggaccgaag aagaaacgga aagtgtaatc tagatgatca gaacggtcag
     5521 cctgctacgt aaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata
     5581 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5641 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5701 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5761 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5821 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5881 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5941 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6001 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6061 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgc
     6121 ctaagaagaa aagaaaggtt tacccatacg acgttccaga ctacgctagt aaggtggaga
     6181 acaaaacaaa gaaactccgg gtgttcgagg cttttgctgg gatcggggcc caacggaagg
     6241 ccttggagaa agtaaggaag gacgagtatg agatcgttgg gcttgctgag tggtatgtcc
     6301 ctgccattgt gatgtatcaa gcgatccaca ataacttcca tactaagctt gagtacaagt
     6361 ccgtgtctag ggaagagatg attgattact tggagaacaa gactctatct tggaatagca
     6421 agaaccccgt gagcaatggc tactggaaga ggaagaagga tgatgaacta aagattatct
     6481 acaacgctat aaagctctca gaaaaggagg ggaatatctt cgacatcagg gatctatata
     6541 agcggactct caaaaacatt gaccttctga catacagctt cccttgccag gatctgtccc
     6601 agcaaggcat tcagaagggt atgaaaagag gatctggcac taggtctgga ctcctgtggg
     6661 agattgaaag ggcactggac tcaactgaga agaacgatct tccaaagtat ctattgatgg
     6721 agaacgttgg cgctttactc cataagaaga acgaggaaga gctaaaccag tggaaacaga
     6781 aacttgagtc tttgggatac caaaactcaa ttgaggtgtt gaacgctgcc gattttggct
     6841 cctctcaggc tagaaggaga gtgttcatga taagcactct taacgagttc gttgaactgc
     6901 ctaaaggcga caagaagcct aagtccatca agaaagttct gaacaagatc gtgtcagaga
     6961 aggacattct gaacaacctg taagctgatt ttctgctatc tttcataata aaggcagcct
     7021 tgcctgaagt cgcaggttga cttcattttt accgggacga tctctcgaag cggatccatg
     7081 aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg caaggcatgg
     7141 aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta
     7201 acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa
     7261 cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat
     7321 ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc
     7381 tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc
     7441 gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc
     7501 ctccgattga ctgagtcgcc cggatgcatc ttcatcggac aggttagacc agacttatcc
     7561 ggataagtct ggtcccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     7621 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     7681 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     7741 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     7801 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     7861 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     7921 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     7981 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     8041 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     8101 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     8161 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     8221 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     8281 catggacgag ctgtacaagt aactagtacg tgctacgaga tttcgattcc accgccgcct
     8341 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8401 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8521 ctagttgtgg tttgtcccgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9361 tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga
     9421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9841 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct tt
//
