LOCUS       sequence-178002-        8203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A mammalian expression plasmid encoding Transportin-1 (TNPO1) with 
            N-terminus double Myc-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Madugula V, Lu L
  TITLE     A ternary complex comprising transportin1, Rab8 and the ciliary 
            targeting signal directs proteins to ciliary membranes.
  JOURNAL   J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15.
  PUBMED    27633000
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2016-10-15"; volume: "129(20)"; pages: "3922-3934. 
            Epub 2016 Sep 15"
COMMENT     Sequence Label: pDMyc-TNPO1
FEATURES             Location/Qualifiers
     source          1..8203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      29..617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     518..537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        828..1207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1208..1419
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1370..1390
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1416..1440
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1580..1712
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1695..1714
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1757..1775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1790..1819
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1826..1855
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4597..4718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4608..4627)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4662..4681
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4904..5359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4991..5010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5201..5222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5476..5833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5476..5667
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5499..5519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5684..5819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5884..6678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5938..5957)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6548..6567
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6742..6790
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6831..6850)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6950..6972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7010..7028)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7096..7200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7201..7269,7270..8061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7419..7438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
       61 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      661 ggccttttgc tcacatggct cgacagatct tcaatattgg ccattagcca tattattcat
      721 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
      781 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt
      841 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      901 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      961 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1021 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1081 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1141 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1201 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     1261 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1321 acgggacttt ccaaaatgtc gtaacaactg cgatcgcccg ccccgttgac gcaaatgggc
     1381 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     1441 actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct
     1501 gacacaacag tctcgaactt aagctgcagt gactctctta aggtagcctt gcagaagttg
     1561 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     1621 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     1681 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1741 ttaaggctag agtacttaat acgactcact ataggctagc ggcaccatgg agcagaaact
     1801 gatctcggag gaggacctgt catcagagca gaaactgatc tcggaggagg acctgctcga
     1861 gatggagtat gagtggaaac ctgacgagca agggcttcag caaatcctgc agctgttgaa
     1921 ggagtcccag tccccagaca ccaccatcca gagaaccgtg caacaaaaac tggaacaact
     1981 taatcagtat ccagacttta acaactactt gatttttgtt cttacaaaat taaaatctga
     2041 agatgaaccc acaagatcat tgagtggtct tatcttgaag aataatgtga aagcacactt
     2101 tcagaacttc ccaaatggtg taacagactt tattaaaagt gaatgtttaa ataatattgg
     2161 tgactcctct cctctgatta gagccactgt tggtattttg atcacaacta tagcctccaa
     2221 gggagaattg cagaattggc ctgacctctt accaaaactc tgtagcctgt tggattctga
     2281 agattataat acctgtgagg gagcatttgg tgcccttcag aagatttgtg aagattctgc
     2341 tgagatttta gacagtgatg ttttagatcg tcctctcaac atcatgattc ccaaattttt
     2401 acagttcttc aagcatagta gtccaaaaat aaggtctcac gctgttgcat gtgtcaatca
     2461 gtttatcatc agtaggactc aagctctaat gttgcacatt gattctttta ttgagaatct
     2521 ctttgcatta gctggtgatg aagaaccaga ggtacggaaa aatgtgtgcc gagcacttgt
     2581 gatgttgctc gaagttcgaa tggatcgcct gcttcctcac atgcataata tagttgagta
     2641 catgctacag aggactcaag atcaagatga aaatgtggct ttagaagcct gtgaattttg
     2701 gctaacttta gctgaacagc caatatgcaa agatgtactc gtaaggcatc ttcctaagtt
     2761 gattcctgtg ttagtgaatg gcatgaagta ctcagacata gatattatcc tacttaaggg
     2821 tgatgttgaa gaagacgaaa cgattcctga tagtgaacag gatatacggc cacgttttca
     2881 ccgatcgagg acggtggctc agcagcatga tgaagatgga attgaagagg aagatgatga
     2941 tgatgatgaa attgatgatg atgatacaat ttctgactgg aatctaagaa aatgttctgc
     3001 tgctgccctg gatgttcttg caaatgtgta tcgtgatgaa ctgctgccac atattttgcc
     3061 ccttttgaaa gaattacttt ttcatcatga atgggttgtt aaagaatcag gcattttggt
     3121 tttaggagca attgctgaag gttgcatgca gggcatgatt ccatacttgc ctgagcttat
     3181 tcctcacctt attcagtgcc tctctgataa aaaggctctt gtgcgttcca taacatgctg
     3241 gactcttagc cgctatgcac actgggtggt cagccagccg ccagacacgt acctgaagcc
     3301 attaatgaca gaattgctaa agcgcatcct ggatagcaac aagagagtac aagaagctgc
     3361 ctgcagtgcc tttgctaccc tagaagagga ggcttgtaca gaacttgttc cttaccttgc
     3421 ttatatactt gataccctgg tctttgcatt tagtaaatac cagcataaga acctgctcat
     3481 tctttacgat gccataggaa cattagcaga ttcagtagga catcatttaa acaaaccaga
     3541 atatattcag atgctaatgc ctccactgat ccagaaatgg aacatgttaa aggatgaaga
     3601 taaagatctc ttccctttac ttgagtgcct atcttcagtt gccacagcac tgcagtctgg
     3661 attccttccg tactgtgaac ctgtgtatca gcgttgtgta aacctagtac agaagactct
     3721 tgcacaagcc atgctaaaca atgctcaacc agatcaatat gaagctccag ataaagattt
     3781 tatgatagtg gctcttgatt tactgagtgg cctggctgaa ggacttggag gcaacattga
     3841 acagctggta gcccgaagta acatcctgac actaatgtat cagtgcatgc aggataaaat
     3901 gccagaagtt cgacagagtt cttttgccct gttaggtgac ctcacaaaag cttgctttca
     3961 gcatgttaag ccttgtatag ctgatttcat gccaatattg ggaaccaacc taaatccaga
     4021 attcatttca gtctgcaaca atgccacatg ggcaattgga gaaatctcca ttcaaatggg
     4081 tatagagatg cagccttata ttcctatggt gttgcaccag cttgtagaaa tcattaacag
     4141 acccaacaca ccaaagacgt tgttagagaa tacagcaata acaattggtc gtcttggtta
     4201 cgtttgtcct caagaggtgg cccccatgct acagcagttt ataagaccct ggtgcacctc
     4261 tctgagaaac ataagagaca atgaggaaaa ggattcagca ttccgtggaa tttgtaccat
     4321 gatcagtgtg aatcccagtg gcgtaatcca agattttata tttttttgtg atgccgttgc
     4381 atcatggatt aacccaaaag atgatctcag agacatgttc tgtaagatcc ttcatggatt
     4441 taaaaatcaa gttggcgatg aaaattggag gcgtttctct gaccagtttc ctcttccctt
     4501 aaaagagcgt cttgcagctt tttatggtgt ttaatctaga gtcgacccgg gcggccgctt
     4561 ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat gagtttggac
     4621 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4681 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4741 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     4801 aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac
     4861 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     4921 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4981 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5041 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5101 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5161 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5221 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5281 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5341 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5401 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     5461 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5761 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5821 aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa ggctagagcc
     5881 accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5941 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6001 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6061 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6121 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6181 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6241 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6301 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6361 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6421 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6481 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6541 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6601 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6661 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     6721 acctgccatc acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     6781 tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag tacaatctgc
     6841 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     6901 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6961 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     7021 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7141 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7201 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7261 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7321 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7381 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7441 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7501 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7561 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7621 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7681 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7741 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7801 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7861 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7921 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7981 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8041 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8101 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cag
//
