LOCUS       sequence-178037-        8254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HUMAN STIM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Sun J, Lu F, He H, Shen J, Messina J, Mathew R, Wang D, Sarnaik AA, 
            Chang WC, Kim M, Cheng H, Yang S
  TITLE     STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates 
            invadopodium formation and melanoma invasion.
  JOURNAL   J Cell Biol. 2014 Nov 24;207(4):535-48. doi: 10.1083/jcb.201407082. 
            Epub 2014 Nov 17.
  PUBMED    25404747
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201407082"; journalName: "J Cell Biol"; date: 
            "2014-11-24- 24"; volume: "207"; issue: "4"; pages: "535-48"
COMMENT     Sequence Label: pLNCX2-STIM1
FEATURES             Location/Qualifiers
     source          1..8254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2650..2679
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2695..2712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             2780..3373
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3476..3498)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3584..3724
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3639..3658
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3739..3756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3910..4498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3990..4009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4669..5460,5461..5529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5292..5311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5530..5634)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5702..5720
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             6292..6467
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    6530..6729
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6930..7346
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7276..7298
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7314..7330
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7390..8184
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7444..7463)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8054..8073
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttggt accatggatg tatgcgtccg tcttgccctg
      601 tggctcctct ggggactcct cctgcaccag ggccagagcc tcagccatag tcacagtgag
      661 aaggcgacag gaaccagctc gggggccaac tctgaggagt ccactgcagc agagttttgc
      721 cgaattgaca agcccctgtg tcacagtgag gatgagaaac tcagcttcga ggcagtccgt
      781 aacatccaca aactgatgga cgatgatgcc aatggtgatg tggatgtgga agaaagtgat
      841 gagttcctga gggaagacct caattaccat gacccaacag tgaaacacag caccttccat
      901 ggtgaggata agctcatcag cgtggaggac ctgtggaagg catggaagtc atcagaagta
      961 tacaattgga ccgtggatga ggtggtacag tggctgatca catatgtgga gctgcctcag
     1021 tatgaggaga ccttccggaa gctgcagctc agtggccatg ccatgccaag gctggctgtc
     1081 accaacacca ccatgacagg gactgtgctg aagatgacag accggagtca tcggcagaag
     1141 ctgcagctga aggctctgga cactgtgctc tttgggcctc ctctcttgac tcgccataat
     1201 cacctcaagg acttcatgct ggtggtgtct atcgttattg gtgtgggcgg ctgctggttt
     1261 gcctatatcc agaaccgtta ctccaaggag cacatgaaga agatgatgaa ggacttggag
     1321 gggttacacc gagctgagca gagtctgcat gaccttcagg aaaggctgca caaggcccag
     1381 gaggagcacc gcacagtgga ggtggagaag gtccatctgg aaaagaagct gcgcgatgag
     1441 atcaaccttg ctaagcagga agcccagcgg ctgaaggagc tgcgggaggg tactgagaat
     1501 gagcggagcc gccaaaaata tgctgaggag gagttggagc aggttcggga ggccttgagg
     1561 aaagcagaga aggagctaga atctcacagc tcatggtatg ctccagaggc ccttcagaag
     1621 tggctgcagc tgacacatga ggtggaggtg caatattaca acatcaagaa gcaaaatgct
     1681 gagaagcagc tgctggtggc caaggagggg gctgagaaga taaaaaagaa gagaaacaca
     1741 ctctttggca ccttccacgt ggcccacagc tcttccctgg atgatgtaga tcataaaatt
     1801 ctaacagcta agcaagcact gagcgaggtg acagcagcat tgcgggagcg cctgcaccgc
     1861 tggcaacaga tcgagatcct ctgtggcttc cagattgtca acaaccctgg catccactca
     1921 ctggtggctg ccctcaacat agaccccagc tggatgggca gtacacgccc caaccctgct
     1981 cacttcatca tgactgacga cgtggatgac atggatgagg agattgtgtc tcccttgtcc
     2041 atgcagtccc ctagcctgca gagcagtgtt cggcagcgcc tgacggagcc acagcatggc
     2101 ctgggatctc agagggattt gacccattcc gattcggagt cctccctcca catgagtgac
     2161 cgccagcgtg tggcccccaa acctcctcag atgagccgtg ctgcagacga ggctctcaat
     2221 gccatgactt ccaatggcag ccaccggctg atcgaggggg tccacccagg gtctctggtg
     2281 gagaaactgc ctgacagccc tgccctggcc aagaaggcat tactggcgct gaaccatggg
     2341 ctggacaagg cccacagcct gatggagctg agcccctcag ccccacctgg tggctctcca
     2401 catttggatt cttcccgttc tcacagcccc agctccccag acccagacac accatctcca
     2461 gttggggaca gccgagccct gcaagccagc cgaaacacac gcattcccca cctggctggc
     2521 aagaaggctg tggctgagga ggataatggc tctattggcg aggaaacaga ctccagccca
     2581 ggccggaaga agtttcccct caaaatcttt aagaagcctc ttaagaagct cgagtctaga
     2641 gggcccttcg aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat
     2701 caccatcacc attgagtttc cttaatggcc taacatcgat aaaataaaag attttattta
     2761 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     2821 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     2881 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     2941 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     3001 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     3061 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     3121 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     3181 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     3241 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     3301 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     3361 ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac
     3421 ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     3481 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     3541 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     3601 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     3661 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     3721 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4321 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4621 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4681 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4741 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4801 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4861 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4921 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4981 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5041 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5101 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5161 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5221 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5281 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5341 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5401 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5461 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5521 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5641 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     5701 aaataggcgt atcacgaggc cctttcgtct tcaagaattc ataccagatc accgaaaact
     5761 gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg gcttctgcct cttagaccac
     5821 tctaccctat tccccacact caccggagcc aaagccgcgg cccttccgtt tctttgcttt
     5881 tgaaagaccc cacccgtagg tggcaagcta gcttaagtaa cgccactttg caaggcatgg
     5941 aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaaa gaaacagctg
     6001 aataccaaac aggatatctg tggtaagcgg ttcctgcccc ggctcagggc caagaacaga
     6061 tgagacagct gagtgatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     6121 cggggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagtgaatc
     6181 atcagatgtt tccagggtgc cccaaggacc tgaaaatgac cctgtacctt atttgaacta
     6241 accaatcagt tcgcttctcg cttctgttcg cgcgcttccg ctctccgagc tcaataaaag
     6301 agcccacaac ccctcactcg gcgcgccagt cttccgatag actgcgtcgc ccgggtaccc
     6361 gtattcccaa taaagcctct tgctgtttgc atccgaatcg tggtctcgct gttccttggg
     6421 agggtctcct ctgagtgatt gactacccac gacgggggtc tttcatttgg gggctcgtcc
     6481 gggatttgga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag
     6541 caacttatct gtgtctgtcc gattgtctag tgtctatgtt tgatgttatg cgcctgcgtc
     6601 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     6661 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     6721 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     6781 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     6841 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     6901 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     6961 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     7021 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     7081 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     7141 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     7201 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     7261 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     7321 cagccctcac tccttctcta ggcgccggaa ttccgatctg atcaagagac aggatgagga
     7381 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7441 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7501 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7561 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7621 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     7681 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7741 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7801 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     7861 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     7921 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     7981 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     8041 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     8101 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     8161 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaatagtaa tcaattacgg
     8221 ggtcattagt tcatagccca tatatggagt tccg
//
