LOCUS       sequence-178040-        7060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express  GATA3 N1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S, Yang S
  TITLE     GATA3 transcription factor abrogates Smad4 transcription 
            factor-mediated fascin overexpression, invadopodium formation, and 
            breast cancer cell invasion.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):36971-82. doi: 
            10.1074/jbc.M113.506535. Epub 2013 Nov 14.
  PUBMED    24235142
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.506535"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "36971-82"
COMMENT     Sequence Label: pLPCX2-HA-GATA3 N1(1-259)
FEATURES             Location/Qualifiers
     source          1..7060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             561..587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             1461..2054
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2157..2179)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    2307..2340
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2400..2433
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2453..2472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2553..2570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2724..3312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2804..2823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3483..4274,4275..4343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4106..4125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4344..4448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4516..4534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5136..5311
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5374..5573
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5774..6190
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6120..6142
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6158..6174
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6287..6886
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6287..6306)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6783..6803
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctatggac tacccttatg atgtgccgga ttatgccgag gtgactgcgg
      601 accagccgcg ctgggtgagc caccatcacc ccgcggtcct caacggtcag cacccagaca
      661 cgcaccaccc gggcctcggc cattcgtaca tggaagctca gtatccgctg acggaagagg
      721 tggacgtact ttttaacatc gatggtcaag gcaaccacgt cccgtcctac tacggaaact
      781 ccgtcagggc tacggtgcag aggtatcctc cgacccacca cgggagccag gtatgccgcc
      841 cgcctctgct gcacggatct ctgccctggc tggatggcgg caaagccctg agcagccacc
      901 acaccgcctc gccctggaac ctcagcccct tctccaagac gtccatccac cacggctctc
      961 cggggcctct gtccgtttac cctccggctt catcctcttc tctggcggcc ggccactcca
     1021 gtcctcatct cttcaccttc ccgcccaccc cgccgaaaga cgtctcccca gacccgtcgc
     1081 tgtccacccc gggatccgcc gggtcggcca ggcaagatga gaaagagtgc ctcaagtatc
     1141 aggtgcagct gccagatagc atgaagctgg agacgtctca ctctcgaggc agcatgacca
     1201 ccctgggtgg ggcctcatcc tcagcccacc accccattac cacctatccg ccctatgtgc
     1261 ccgagtacag ctctggactc ttcccaccca gcagcctgct gggaggatcc cctaccgggt
     1321 tcggatgtaa gtcgaggccc aaggcacgat ccagcacaga ataggaattc gttaggccat
     1381 taaggcctgt cgacaagcgg ccgcctcggc caaacatcga taaaataaaa gattttattt
     1441 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     1501 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     1561 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     1621 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     1681 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     1741 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     1801 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     1861 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     1921 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     1981 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     2041 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     2101 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2161 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2221 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     2281 tcacgtagcg atagcggagt gtactgataa cttcgtataa tgtatgctat acgaagttat
     2341 taggtctgaa gaggagttta cgtccagcca agctagggcc gcgatccgga acccttaata
     2401 taacttcgta taatgtatgc tatacgaagt tatcagtact ggcttaacta tgcggcatca
     2461 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2521 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3121 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3781 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4081 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4441 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4501 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attgctagca
     4561 attgctagca attgctagca attcatacca gatcaccgaa aactgtcctc caaatgtgtc
     4621 cccctcacac tcccaaattc gcgggcttct gcctcttaga ccactctacc ctattcccca
     4681 cactcaccgg agccaaagcc gcggcccttc cgtttctttg cttttgaaag accccacccg
     4741 taggtggcaa gctagcttaa gtaacgccac tttgcaaggc atggaaaaat acataactga
     4801 gaatagaaaa gttcagatca aggtcaggaa caaagaaaca gctgaatacc aaacaggata
     4861 tctgtggtaa gcggttcctg ccccggctca gggccaagaa cagatgagac agctgagtga
     4921 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcggggc caagaacaga
     4981 tggtccccag atgcggtcca gccctcagca gtttctagtg aatcatcaga tgtttccagg
     5041 gtgccccaag gacctgaaaa tgaccctgta ccttatttga actaaccaat cagttcgctt
     5101 ctcgcttctg ttcgcgcgct tccgctctcc gagctcaata aaagagccca caacccctca
     5161 ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc ccaataaagc
     5221 ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc tcctctgagt
     5281 gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt tggagacccc
     5341 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
     5401 gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact agttagctaa
     5461 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
     5521 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
     5581 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
     5641 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
     5701 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     5761 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     5821 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     5881 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     5941 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     6001 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     6061 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     6121 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     6181 tctaggcgcc ggaatgatcc cccgggctgc aggtcggccg ccacgaccgg tgccgccacc
     6241 atcccctgac ccacgcccct gacccctcac aaggagacga ccttccatga ccgagtacaa
     6301 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     6361 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     6421 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6481 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6541 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6601 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6661 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6721 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6781 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6841 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     6901 cccgcagcgc ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccacccggg
     6961 gcggccccgc cgaccccgca cccgcccccg aggcccaccg actctagtaa tagtaatcaa
     7021 ttacggggtc attagttcat agcccatata tggagttccg
//
