LOCUS       sequence-178041-        7165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GATA3 N2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S, Yang S
  TITLE     GATA3 transcription factor abrogates Smad4 transcription 
            factor-mediated fascin overexpression, invadopodium formation, and 
            breast cancer cell invasion.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):36971-82. doi: 
            10.1074/jbc.M113.506535. Epub 2013 Nov 14.
  PUBMED    24235142
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.506535"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "36971-82"
COMMENT     Sequence Label: pLPCX2-HA-GATA3 N2(1-294)
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             561..587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             1566..2159
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    2412..2445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2505..2538
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2558..2577
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2658..2675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2829..3417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2909..2928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3588..4379,4380..4448))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4211..4230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4449..4553)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4621..4639
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5241..5416
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5479..5678
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5879..6295
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6225..6247
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6263..6279
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6392..6991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6392..6411)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6888..6908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctatggac tacccttatg atgtgccgga ttatgccgag gtgactgcgg
      601 accagccgcg ctgggtgagc caccatcacc ccgcggtcct caacggtcag cacccagaca
      661 cgcaccaccc gggcctcggc cattcgtaca tggaagctca gtatccgctg acggaagagg
      721 tggacgtact ttttaacatc gatggtcaag gcaaccacgt cccgtcctac tacggaaact
      781 ccgtcagggc tacggtgcag aggtatcctc cgacccacca cgggagccag gtatgccgcc
      841 cgcctctgct gcacggatct ctgccctggc tggatggcgg caaagccctg agcagccacc
      901 acaccgcctc gccctggaac ctcagcccct tctccaagac gtccatccac cacggctctc
      961 cggggcctct gtccgtttac cctccggctt catcctcttc tctggcggcc ggccactcca
     1021 gtcctcatct cttcaccttc ccgcccaccc cgccgaaaga cgtctcccca gacccgtcgc
     1081 tgtccacccc gggatccgcc gggtcggcca ggcaagatga gaaagagtgc ctcaagtatc
     1141 aggtgcagct gccagatagc atgaagctgg agacgtctca ctctcgaggc agcatgacca
     1201 ccctgggtgg ggcctcatcc tcagcccacc accccattac cacctatccg ccctatgtgc
     1261 ccgagtacag ctctggactc ttcccaccca gcagcctgct gggaggatcc cctaccgggt
     1321 tcggatgtaa gtcgaggccc aaggcacgat ccagcacaga aggcagggag tgtgtgaact
     1381 gcggggcaac ctctacccca ctgtggcggc gagatggtac cgggcactac ctttgcaatg
     1441 cctgcggact ctaccataaa atgaattagg aattcgttag gccattaagg cctgtcgaca
     1501 agcggccgcc tcggccaaac atcgataaaa taaaagattt tatttagtct ccagaaaaag
     1561 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     1621 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     1681 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     1741 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     1801 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     1861 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     1921 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     1981 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     2041 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     2101 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     2161 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
     2221 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     2401 ggagtgtact gataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga
     2461 gtttacgtcc agccaagcta gggccgcgat ccggaaccct taatataact tcgtataatg
     2521 tatgctatac gaagttatca gtactggctt aactatgcgg catcagagca gattgtactg
     2581 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     2641 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2701 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2761 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2821 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2881 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2941 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3001 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3061 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3121 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3181 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3241 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3301 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3361 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3901 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4201 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4561 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4621 aataggcgta tcacgaggcc ctttcgtctt caagaattgc tagcaattgc tagcaattgc
     4681 tagcaattca taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca
     4741 aattcgcggg cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca
     4801 aagccgcggc ccttccgttt ctttgctttt gaaagacccc acccgtaggt ggcaagctag
     4861 cttaagtaac gccactttgc aaggcatgga aaaatacata actgagaata gaaaagttca
     4921 gatcaaggtc aggaacaaag aaacagctga ataccaaaca ggatatctgt ggtaagcggt
     4981 tcctgccccg gctcagggcc aagaacagat gagacagctg agtgatgggc caaacaggat
     5041 atctgtggta agcagttcct gccccggctc ggggccaaga acagatggtc cccagatgcg
     5101 gtccagccct cagcagtttc tagtgaatca tcagatgttt ccagggtgcc ccaaggacct
     5161 gaaaatgacc ctgtacctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     5221 gcgcttccgc tctccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     5281 ttccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
     5341 tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg
     5401 acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc
     5461 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
     5521 gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg
     5581 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
     5641 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
     5701 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
     5761 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     5821 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     5881 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     5941 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     6001 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     6061 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     6121 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     6181 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     6241 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
     6301 gatcccccgg gctgcaggtc ggccgccacg accggtgccg ccaccatccc ctgacccacg
     6361 cccctgaccc ctcacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
     6421 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     6481 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     6541 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6601 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6661 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6721 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6781 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6841 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6901 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6961 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     7021 cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc
     7081 ccgcacccgc ccccgaggcc caccgactct agtaatagta atcaattacg gggtcattag
     7141 ttcatagccc atatatggag ttccg
//
