LOCUS       sequence-178043-        6839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GATA3 C1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S, Yang S
  TITLE     GATA3 transcription factor abrogates Smad4 transcription 
            factor-mediated fascin overexpression, invadopodium formation, and 
            breast cancer cell invasion.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):36971-82. doi: 
            10.1074/jbc.M113.506535. Epub 2013 Nov 14.
  PUBMED    24235142
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.506535"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "36971-82"
COMMENT     Sequence Label: pLPCX2-HA-GATA3 C1(259-443)
FEATURES             Location/Qualifiers
     source          1..6839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             561..587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             1240..1833
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1936..1958)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    2086..2119
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2179..2212
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2232..2251
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2332..2349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2503..3091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2583..2602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3262..4053,4054..4122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3885..3904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4123..4227)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4295..4313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4915..5090
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5153..5352
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5553..5969
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5899..5921
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5937..5953
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6066..6665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6066..6085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6562..6582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctatggac tacccttatg atgtgccgga ttatgccgaa ggcagggagt
      601 gtgtgaactg cggggcaacc tctaccccac tgtggcggcg agatggtacc gggcactacc
      661 tttgcaatgc ctgcggactc taccataaaa tgaatgggca gaaccggccc cttatcaagc
      721 ccaagcgaag gctgtcggca gcaaggagag cagggacatc ctgcgcgaac tgtcagacca
      781 ccaccaccac cctctggagg aggaacgcta atggggaccc ggtctgcaat gcctgtgggc
      841 tgtactacaa gcttcataat attaacagac ccctgactat gaagaaagaa ggcatccaga
      901 cccgaaaccg gaagatgtct agcaaatcga aaaagtgcaa aaaggtgcat gacgcgctgg
      961 aggacttccc caagagcagc tccttcaacc cggccgctct ctccagacac atgtcatccc
     1021 tgagccacat ctctcccttc agccactcca gccacatgct gaccacaccg acgcccatgc
     1081 atccgccctc cggcctctcc ttcggacctc accacccttc cagcatggtc accgccatgg
     1141 gtagaattcg ttaggccatt aaggcctgtc gacaagcggc cgcctcggcc aaacatcgat
     1201 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     1261 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     1321 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
     1381 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
     1441 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     1501 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
     1561 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     1621 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     1681 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     1741 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     1801 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
     1861 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
     1921 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1981 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2041 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tactgataac ttcgtataat
     2101 gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa gctagggccg
     2161 cgatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt atcagtactg
     2221 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2281 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     2341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2941 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3241 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3301 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3361 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3421 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3481 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3541 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     3601 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3661 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3721 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3781 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3841 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     3901 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3961 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4021 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4081 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4261 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4321 tcttcaagaa ttgctagcaa ttgctagcaa ttgctagcaa ttcataccag atcaccgaaa
     4381 actgtcctcc aaatgtgtcc ccctcacact cccaaattcg cgggcttctg cctcttagac
     4441 cactctaccc tattccccac actcaccgga gccaaagccg cggcccttcc gtttctttgc
     4501 ttttgaaaga ccccacccgt aggtggcaag ctagcttaag taacgccact ttgcaaggca
     4561 tggaaaaata cataactgag aatagaaaag ttcagatcaa ggtcaggaac aaagaaacag
     4621 ctgaatacca aacaggatat ctgtggtaag cggttcctgc cccggctcag ggccaagaac
     4681 agatgagaca gctgagtgat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4741 gctcggggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagtga
     4801 atcatcagat gtttccaggg tgccccaagg acctgaaaat gaccctgtac cttatttgaa
     4861 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ccgctctccg agctcaataa
     4921 aagagcccac aacccctcac tcggcgcgcc agtcttccga tagactgcgt cgcccgggta
     4981 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt
     5041 gggagggtct cctctgagtg attgactacc cacgacgggg gtctttcatt tgggggctcg
     5101 tccgggattt ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     5161 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc
     5221 gtctgtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     5281 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     5341 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     5401 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     5461 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     5521 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     5581 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     5641 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     5701 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     5761 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     5821 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     5881 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     5941 atccagccct cactccttct ctaggcgccg gaatgatccc ccgggctgca ggtcggccgc
     6001 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     6061 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6121 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6181 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6241 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6301 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6361 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6421 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6481 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6541 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6601 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6661 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     6721 ccgaccgaag ccacccgggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     6781 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccg
//
