LOCUS       sequence-178044-        6731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GATA3 C2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S, Yang S
  TITLE     GATA3 transcription factor abrogates Smad4 transcription 
            factor-mediated fascin overexpression, invadopodium formation, and 
            breast cancer cell invasion.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):36971-82. doi: 
            10.1074/jbc.M113.506535. Epub 2013 Nov 14.
  PUBMED    24235142
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.506535"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "36971-82"
COMMENT     Sequence Label: pLPCX2-HA-GATA3 C1(295-443)
FEATURES             Location/Qualifiers
     source          1..6731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             561..587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             1132..1725
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1828..1850)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    1978..2011
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2071..2104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2124..2143
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2224..2241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2395..2983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2475..2494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3154..3945,3946..4014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3777..3796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4015..4119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4187..4205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4807..4982
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5045..5244
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5445..5861
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5791..5813
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5829..5845
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5958..6557
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5958..5977)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6454..6474
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctatggac tacccttatg atgtgccgga ttatgccggg cagaaccggc
      601 cccttatcaa gcccaagcga aggctgtcgg cagcaaggag agcagggaca tcctgcgcga
      661 actgtcagac caccaccacc accctctgga ggaggaacgc taatggggac ccggtctgca
      721 atgcctgtgg gctgtactac aagcttcata atattaacag acccctgact atgaagaaag
      781 aaggcatcca gacccgaaac cggaagatgt ctagcaaatc gaaaaagtgc aaaaaggtgc
      841 atgacgcgct ggaggacttc cccaagagca gctccttcaa cccggccgct ctctccagac
      901 acatgtcatc cctgagccac atctctccct tcagccactc cagccacatg ctgaccacac
      961 cgacgcccat gcatccgccc tccggcctct ccttcggacc tcaccaccct tccagcatgg
     1021 tcaccgccat gggtagaatt cgttaggcca ttaaggcctg tcgacaagcg gccgcctcgg
     1081 ccaaacatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     1141 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     1201 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     1261 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     1321 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     1381 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     1441 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     1501 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     1561 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     1621 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     1681 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg
     1741 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct
     1801 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     1861 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     1921 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtactgata
     1981 acttcgtata atgtatgcta tacgaagtta ttaggtctga agaggagttt acgtccagcc
     2041 aagctagggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     2101 ttatcagtac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     2161 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2821 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4201 gaggcccttt cgtcttcaag aattgctagc aattgctagc aattgctagc aattcatacc
     4261 agatcaccga aaactgtcct ccaaatgtgt ccccctcaca ctcccaaatt cgcgggcttc
     4321 tgcctcttag accactctac cctattcccc acactcaccg gagccaaagc cgcggccctt
     4381 ccgtttcttt gcttttgaaa gaccccaccc gtaggtggca agctagctta agtaacgcca
     4441 ctttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga
     4501 acaaagaaac agctgaatac caaacaggat atctgtggta agcggttcct gccccggctc
     4561 agggccaaga acagatgaga cagctgagtg atgggccaaa caggatatct gtggtaagca
     4621 gttcctgccc cggctcgggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     4681 agtttctagt gaatcatcag atgtttccag ggtgccccaa ggacctgaaa atgaccctgt
     4741 accttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttccgctctc
     4801 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcttcc gatagactgc
     4861 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggtc
     4921 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccacgacgg gggtctttca
     4981 tttgggggct cgtccgggat ttggagaccc ctgcccaggg accaccgacc caccaccggg
     5041 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgtttgatg
     5101 ttatgcgcct gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg
     5161 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg
     5221 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat
     5281 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     5341 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt
     5401 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     5461 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     5521 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     5581 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     5641 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     5701 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     5761 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     5821 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaatgatc ccccgggctg
     5881 caggtcggcc gccacgaccg gtgccgccac catcccctga cccacgcccc tgacccctca
     5941 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6001 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6061 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     6481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     6541 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     6601 gaccccatgg ctccgaccga agccacccgg ggcggccccg ccgaccccgc acccgccccc
     6661 gaggcccacc gactctagta atagtaatca attacggggt cattagttca tagcccatat
     6721 atggagttcc g
//
