LOCUS       sequence-178050-        5643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Mouse Nocturnin with 3x Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5643)
  TITLE     Carla Green lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-mNoc-3X FLAG
FEATURES             Location/Qualifiers
     source          1..5643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1948..1995
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2002..2025
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2078..2097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2079..2097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2371..2492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2408..2427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2462..2481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2499..2954)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2636..2657)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2848..2867
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2981..3085
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3087..3444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3087..3278
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3110..3130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3295..3430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3479..4273
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3479..4273
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3533..3552)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4143..4162
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4461..4480)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4505..4552
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4881..5469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5370..5389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      601 gcggccgctc tagcccgggc ggatcccccg ggctgcagga attcgccctt attcatgtat
      661 cagagcccgc ggcggctctg ctcggccctg ctgctcaggg acgcccccgg cctgcgccgc
      721 acactcgtgc ccggcccgcg ccgaacgctc gccccgccgg tgctcggatc tcggcccaag
      781 tccccgcaac tgcaggcggc ggcggcctcg ggcgccgcga ggtcgcgtcc ccgaacagtg
      841 agttccatgg gaaacggcac cagtcgactc tacagtgctc tcgccaagac cgtcaacagc
      901 agtgcagctg ctcagcaccc ggagtacttg gtgtcaactg accccgaaca tctggagccc
      961 atcgatccta aggaacttct ggaggaatgc agggctgttc tgcacactcg gccaccccgc
     1021 taccagcggg attttgtgga cctgaggaca gattgctcca gcagccactc tcccattcgc
     1081 gtcatgcagt ggaacatcct cgcccaagct ctcggagaag gcaaagacaa ctttgtgcag
     1141 tgccccgtgg aagcgctcaa atgggaagag aggaagtgcc tgatcctgga ggagatcctg
     1201 gcttaccagc cagacatact gtgcctccag gaagtggacc actactttga caccttccag
     1261 ccactcctca gtagactggg ctaccaaggc acgtttttcc ccaagccctg gtcaccatgt
     1321 ctagatgtgg aacacaacaa cggtccagat ggctgtgcct tattttttct ccaaaaccga
     1381 ttcaagctta tcagcagcac caatattagg ctgacagcca tgaccctaaa aaccaatcag
     1441 gtggccatcg cacagaccct ggagtgcaag gagtctgggc gacagttctg cattgctgtc
     1501 acccacttaa aagcccgcac tggctgggag cggttccggt cagctcaggg ctgtgacctc
     1561 ctccagaacc tgcagaacat cacccaggga gcaaagatcc ccctgatcgt ctgcggggac
     1621 ttcaacgcag agccaaccga agaggtctac aaacactttg cgtcctccag cctcaacctc
     1681 aacagcgcct acaagctgct gagtcccgat ggacagtcgg agcctcccta cacaacctgg
     1741 aagatccgga cctcaggcga gtgtcgccac acgctggact atatctggta ctccagacat
     1801 gctctgagtg tcacgtccgc cctggatcta ctcactgaag aacagattgg gcccaaccgg
     1861 ctcccatcct tccattaccc ctcggaccac ctgtccctgg tgtgtgactt cagctttaac
     1921 gaggagcccc atgagctctt tctcgaggat tacaaggatg acgacgataa ggattacaag
     1981 gatgacgacg ataaggtcga ggattacaag gatgacgacg ataagtaggg cccggtacct
     2041 taattaatta aggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa
     2101 ttcactcgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga gatccaattt
     2161 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattcacagt
     2221 cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat
     2281 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2341 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2461 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     2521 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2581 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     2641 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2701 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     2761 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     2821 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     2881 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     2941 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3061 tcaataatat tgaaaaagga agaatcctga ggcggaaaga accagctgtg gaatgtgtgt
     3121 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3181 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3241 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3301 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3361 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3421 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3481 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3541 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3601 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3661 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3721 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3781 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3841 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3901 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3961 acatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4021 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4081 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4141 agcgttggct acccgtgata ttgctgaaga acttggcggc gaatgggctg accgcttcct
     4201 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4261 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4321 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4381 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4441 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4501 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     4561 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     4621 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     4681 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     4741 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4801 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4861 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4921 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4981 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5041 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5101 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5461 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5521 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     5581 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     5641 ccg
//
