LOCUS       sequence-178054-        5395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus Cry2b cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  TITLE     Carla Green lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Xcry2b
FEATURES             Location/Qualifiers
     source          1..5395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     75..91
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..91
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2608..2626)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2647..2663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2647..2663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2660..2682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2671..2687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2695..2701,2702..2719,2720..2725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2740..2761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2878..2895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3049..3637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3129..3148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3808..4599,4600..4668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4431..4450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4669..4773)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4799..5254)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4936..4957)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5148..5167
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(5381..5395,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5395,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctccacc
       61 gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc ggcacgagga
      121 aacacaagcg gtaccagggt ggagaggagc ggcctgacat ggagggtaaa ccctcggtga
      181 gctcggtgca ttggttccgg aagggactga gactacacga taacccagcc ttgttagctg
      241 ccctgcgcgg tgctaactcc gtgcgatgtg tctacatcct ggatccttgg tttgcggcct
      301 cctcctcagg gggagtcaat cgctggaggt ttctcttgca gtccttggag gatctggaca
      361 gcagtttaag gaagctcaac tctcgcctgt ttgtagttcg agggcaaccg gcagacgtct
      421 tccccaagct gtttaaggag tggggtgtct cccgcctcac cttcgagtac gattcggagc
      481 ctttcgggaa ggagcgagat gcggtcatta tgaagctggc gaaagaagcc ggagtggagg
      541 tgatcgtgga gaactcgcat actctgtatg acttggacag ggttattgaa ctgaacggac
      601 atagcccccc tctcacgtac aagcggttcc aggccatcat cagccgcatg gagcttcctc
      661 gtcggccggc ccctagtgtc acccgccagc agatggaggc ttgccgagca gagatcaagc
      721 gcaatcatga tgaaacatat ggggtgccat ctcttgagga gttgggattc cacagtgaaa
      781 ataagggaca cgctatttgg ccaggaggag aaactgaagc gctggcgcgt ctagacagac
      841 acctggagcg taaggcttgg gtggcaaact acgagaggcc cagaatgagt gccaactccc
      901 tgttagcaag tcctacgggc ctcagtcctt acctgcgatt tggatgcttg tcgtgtcgac
      961 tcttctatta ccgcctacag gagttatacc agaaggtgaa gaaaaacagc cctcccccat
     1021 tatccttgta cgggcagctg ctttggcgag agttcttcta cacggccgcc actaacaacc
     1081 ccaaatttga ccaaatggaa gggaacccaa tctgtgtgca gatcccctgg gacaagaacc
     1141 cgaaggctct ggccaagtgg acagaaggaa agacggggtt cccttggatc gacgccatta
     1201 tgacgcagct caggcaggag ggctggatac accacttggc gaggcacgcg gttgcatgtt
     1261 tcctcacccg gggggatctg tggaacagct gggagtgtgg cgtaaaggtc ttcgatgaat
     1321 tgttgctgga cgcagatttt agcgtgaatg ccggcagctg gatgtggctc tcgtgcagcg
     1381 cctttttcca gcaattcttc cactgttatt gtcccgtggg ctttggaagg cgaacagatc
     1441 caagtgggga ctatgtaaaa cggtaccttc ccgtgctgaa ggcgttccca tcccgctaca
     1501 tatatgagcc ttggagtgcc ccagagtctg tacagaaaga agccaagtgc attattggta
     1561 ttgactaccc aaagcccatc gtaaaccacg ccgaggcgag ccggatgaat atagagcgca
     1621 tgaaacagac ctaccagcag ctgtcccact atagaggcct ctgtatcttg gcttctgtac
     1681 catcctgtgt ggaagacctg ggaggtcccg taacagactc cagccacaac ccagcggaag
     1741 cagctcctaa acagtctctg tgcaatgccg actcccccaa acgcaaactg gaggggtcag
     1801 aagaagcgtc gcacgtgaag gtcagggtgc ggagtgtccc ggtgatgagg aggccagaga
     1861 acgacttctg agtgtcgcat ctgatgagct gctgaaacag agagaaacct gcacctgtaa
     1921 cagcattagg actagtggga ggctgcactt cctgaagata cctgcgttcc tagaggggcc
     1981 ccctgaatat atttaatctc ttggctccaa gacaagcaac aatagtatta ttactggcat
     2041 ctctgtcagc cctagttttc tccaaccttt ggcttggtat ttaaacaagt gtctgctcct
     2101 atatctgttt aagaggtgta aatcaggctg ctgcggaact acagcacgtc ccctgaggat
     2161 gatgggagtt gtagttcccc aagcacttta gcgcttctgg ctggagagct ctggtgtaga
     2221 tggtggagga tatttcaacc ctaaacccag cgaatccgct ccggtagcaa ccctgggctg
     2281 catctgacaa tataaaaaaa aaatatatat atatatatct atatatatat atatatatct
     2341 atctatatat atatatctac tagaggctta ctcattgacg gtgcaagagg agctcatact
     2401 cattctgcct cggcagggga ggggttaatt ttgcatgttt tagcagataa aaaagctgct
     2461 gacgtgtcac cagtcgtatg tgtacattct gcccatgtat gtaaatacag atgctgtttg
     2521 tatataataa atgtaatttt aaatactctt caaaaaaaaa aaaaaaaaac tcgacctcga
     2581 gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     2641 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2701 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2761 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2941 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3001 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3061 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3481 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     4801 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4861 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4921 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4981 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     5041 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     5101 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     5161 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     5221 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     5281 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     5341 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgtt
//
