LOCUS       sequence-178070-        8021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a L
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(861..872,873..1035)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1414..1538
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1563..1568,1569..1586,1587..1593)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1647..2306
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1713..1732)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2648..2953
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2832..2851
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2994..3118)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          4898..5470
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      5544..5553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5550..5552,5553..5555,5556..6269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5550..5552,5553..5555,5556..6266)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5595..5616)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5856..5875)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6203..6224
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6314..6448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6338..6357)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6392..6411
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6885..7072,7073..7084)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7118..7134)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7118..7134)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7171..7191)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7171..7189)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7210..7226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7210..7226)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7223..7245)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7234..7250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7258..7264,7265..7282,7283..7288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7303..7324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7441..7458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7692..7711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcaatagct atattttaca tctccttttt ttgctgtctt ttaattagcc ttgttttgct
       61 gttatcttga ttgaaactgt aacttcttca cctgcttctt ttctttgtag gttttgccag
      121 cccagaaaca ggcatggctg tgcaaggcca gagcaccatg cacaatgcgc tgcatgtctt
      181 catgaacggc tcaatgtcct cagtccaggg ctcagccaac gaccccatct tcctccttca
      241 ccatgctttc attgacaggt aacaaacacg tcatgacatt agactgcaca gtttttgaca
      301 aagttcatac aatctgttgt ttatagctgc tacaattagt gaagtttgtg aatgtacttg
      361 gatgagcagc gaaagatcaa ttgagatcaa ttgttagagt ttggttgccc tgcagagcaa
      421 agaacaaaaa ataatctggt ggctttactg cgtgaggtta ttattggtgg aatagaaaca
      481 caaaacataa ttgcatttat ttgtttaatt ttttatctta tcttaacttt catcttgcat
      541 atttgtttct tacatcattt ctagcatctt tgagcgctgg ctaagaactc atcagcctcc
      601 ccggtccatc tacccacgta ccaatgcacc aattggccac aatgacggct actacatggt
      661 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg ctcttggata
      721 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      781 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      841 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      901 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      961 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
     1021 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
     1081 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
     1141 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
     1201 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
     1261 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     1321 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     1381 gaaacacagg ccagatgggc cctcgaggat atcacaagtt tgtacaaaaa agctgaacga
     1441 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     1501 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     1561 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     1621 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1681 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1741 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1801 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1861 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1921 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1981 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     2041 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     2101 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     2161 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     2221 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     2281 gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac
     2341 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     2401 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     2461 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     2521 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     2581 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     2641 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     2701 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     2761 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     2821 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     2881 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     2941 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     3001 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     3061 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga
     3121 tatcaagatc tgtcagttta cctcaagcta cagggggctt agcaatcagg ccttaaaaaa
     3181 gcactgacag gttaacctgc tgtgcacagg ggtctctagg gacaggcctc cactacagtt
     3241 ttacatgcaa cgcttctgtt tgccaaccag agactcagca cataatagag ttgttaccag
     3301 ccacttgagt ttaacctttg cagaccctag gaaaaattga catgctctaa actgctttat
     3361 gctctttcta gttccagctt actttgttta ttttgttaag taatgtgaca gggaatgtca
     3421 aacagatgca ttgcagtttt aggaaccaat gaaaaatggc aatgtagttg aacttatcaa
     3481 aatatagtag tttaatcatc ttactgtaac tcgaatggag atttttaaat attttaacta
     3541 aatcatcatt gttaactaac tagtttgcca ggtttaaaca tttcctgtgc tgtaatttac
     3601 aacctattac acgcgatctt acaaatgcat ccattttact atggtcatgt cttagtaatg
     3661 ggcttttagt gagacagttt agggttttca cattttgaag acagtctaca acaatagtca
     3721 gcacatcaac atgcttgtcg aaggtgaaca cagcactcta agaggctctt tattgttcgt
     3781 tctctaacaa gccattgtgg gccacagaaa atgtaaaacg tcttccatag atatcaatcg
     3841 aagaatttat cgttacatct ctaaaaagtt aagctccttt ttgataggtt attgccttat
     3901 tctgtatttc acccagcctg gattgtactg caatatgctt tttcatgctt agacaagagt
     3961 attcggtaat gacaatcact gagcattact aacgactagc cattagagcc gtaaccacgg
     4021 agcgttccaa actcctcctg tgtagcacaa tggaactgca tctatctgag agatacaagt
     4081 agaattaaca tttattgttt gcgacagatt ttgcaggcgt gctccttcat caaatgtttt
     4141 tagaccttaa cagatttgac aggaatttgt aatcttgata cctccacaaa tctctattga
     4201 taacgctgac aatgtcagta tatttatatt tacattttct tgcatcagat gccccacatt
     4261 aattctatct atttcttttt ttccagttac aagattgaca aattttcatg ttcagcataa
     4321 tttgtttaat ccatgactta aatgtcaaaa taatcttgac agtcttttcc tagagaaata
     4381 tattctatga tagtagacta taattaggca tgtgatgata attgttttca aggtataccg
     4441 cggtttggaa aagccaaggt tttacaactg tcaacatacg gtatatgtaa gatatgtata
     4501 agtaagattg tatgtatatg tatattaaat tgtttttgtt tggtttttta gaacaacaaa
     4561 agatatccaa agatgccttt ttaaaatgta aagaaatctg tttttgaaac taatgaagac
     4621 agaagtcaat gaatcatttg aattacttag cctcacatgt ttacggttcc aaaatatttt
     4681 aaatgcttct taaaataaaa tatatactgt gttcaaaggg gaagaagttt tgttttttac
     4741 tcagacattt aaagagaata tattttagat ccgtaatcac aatgccatcg aacaggtaga
     4801 tctctcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc
     4861 ctccacacga atttcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
     4921 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     4981 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     5041 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     5101 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     5161 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     5221 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     5281 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     5341 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     5401 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     5461 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
     5521 attcctcgac ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     5581 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5641 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5701 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5761 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5821 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5881 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5941 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     6001 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     6061 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6121 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6181 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6241 ctctcggcat ggacgagctg tacaagtaaa gcggccgcca ccgcggtgga gctcgaatta
     6301 attcatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
     6361 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6421 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6481 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     6541 ggctgattat gatcctctag atcagatccg aagatacggc cacgggtgct cttgatcctg
     6601 tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt
     6661 cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg
     6721 gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca
     6781 tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc
     6841 agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa
     6901 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
     6961 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
     7021 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
     7081 tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc
     7141 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     7201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7981 tagcagagcg aggtatgtag gcggtgctac agagttcttg a
//
