LOCUS       sequence-178071-        9223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Chen F, LoTurco J
  TITLE     A method for stable transgenesis of radial glia lineage in rat 
            neocortex by piggyBac mediated transposition.
  JOURNAL   J Neurosci Methods. 2012 Jun 15;207(2):172-80. Epub 2012 Apr 11.
  PUBMED    22521325
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2012-06-15"; volume: "207(2)"; pages: "172-80. 
            Epub 2012 Apr 11"
COMMENT     Sequence Label: PBCAG-EGFR
FEATURES             Location/Qualifiers
     source          1..9223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   12..74
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    371..404
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        500..803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        805..1080
                     /label=chicken beta-actin promoter
     intron          1081..2089
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2013..2035
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2097..2116
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(2160..2231,2232..4094,4095..4163,4164..5792)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKISSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     primer_bind     complement(5878..5897)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5943..5998
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5997..6016)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   6669..6703
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6726..6742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6726..6742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6739..6761)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6750..6766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6774..6780,6781..6798,6799..6804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6819..6840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6899..7094
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6945..7080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7100..7234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7137..7156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7191..7210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7302..7319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7473..8061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7553..7572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8232..9023,9024..9092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9093..9197)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgactta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt
       61 aaaattgacg catgtgtttt atcgatctgt atatcgaggt ttatttatta atttgaatag
      121 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
      181 ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
      241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc
      301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact
      361 tattaatcgc ataacttcgt ataatgtatg ctatacgaag ttatgcgatt aagggatctg
      421 tagggcgcag tagtccaact agttattaat agtaatcaat tacggggtca ttagttcata
      481 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      541 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      601 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      661 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      721 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      781 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      841 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      901 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      961 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     1021 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     1081 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
     1141 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     1201 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
     1261 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt
     1321 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc
     1381 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg
     1441 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1501 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc
     1561 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg
     1621 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1681 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg
     1741 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1801 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct
     1861 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1921 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga
     1981 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     2041 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     2101 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtacca
     2161 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg
     2221 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt
     2281 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg
     2341 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga
     2401 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt
     2461 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag
     2521 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac
     2581 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga
     2641 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc
     2701 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg
     2761 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg
     2821 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca
     2881 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca
     2941 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc
     3001 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg
     3061 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag
     3121 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag
     3181 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa
     3241 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct
     3301 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa
     3361 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg
     3421 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg
     3481 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg
     3541 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt
     3601 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg
     3661 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca
     3721 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc
     3781 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag
     3841 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc
     3901 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg
     3961 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc
     4021 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc
     4081 ctaagatctc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg
     4141 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga
     4201 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc
     4261 aagctctctt gaggatcttg aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg
     4321 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc
     4381 ccgtcgctat caaggaatta agagaagcaa catctccgaa ggccaacaag gaaatcctcg
     4441 atgaagccta cgtgatggcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct
     4501 gcctcacctc caccgtgcaa ctcatcacgc agctcatgcc cttcggctgc ctcctggact
     4561 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga
     4621 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca
     4681 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac
     4741 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga
     4801 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg
     4861 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca
     4921 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca
     4981 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt
     5041 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca
     5101 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc
     5161 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc
     5221 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa
     5281 ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca
     5341 aggaagacag cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca
     5401 gcatagacga caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc
     5461 ccgctggctc tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca
     5521 gagacccaca ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca
     5581 ctgtccagcc cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag
     5641 gcagccacca aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag
     5701 ccaagccaaa tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg
     5761 cgccacaaag cagtgaattt attggagcat gagcggccgc actcctcagg tgcaggctgc
     5821 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     5881 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     5941 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     6001 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     6061 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     6121 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     6181 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     6241 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     6301 tgtccctctt ctcttatgga gatccctcga cctgcagagc tccagcctac acaatcgctc
     6361 aagacgtgta atgcttttat tatatattag tcacgatatc tataacaaga aaatatatat
     6421 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     6481 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     6541 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     6601 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     6661 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaaaag cttggcgtaa
     6721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     6901 atctcaataa gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7021 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7081 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7201 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     7261 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7321 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7381 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7501 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7561 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7621 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7681 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7741 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7801 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7861 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7921 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7981 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8041 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8101 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8161 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8221 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8281 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8341 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8401 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8461 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8521 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8581 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8641 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8701 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8761 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8821 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8881 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8941 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9001 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9061 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9121 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9181 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
