LOCUS       sequence-178077-        3700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MultiSite Gateway entry clones, second fragment (attL5, attL2) Human
            FOS CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner
            E, Nelson PJ
  TITLE     A vector platform for the rapid and efficient engineering of stable 
            complex transgenes.
  JOURNAL   Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
  PUBMED    27694838
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-3"; volume: "6:34365. doi"; pages: " 
            10.1038/srep34365"
COMMENT     Sequence Label: pENTR221-FOS
FEATURES             Location/Qualifiers
     source          1..3700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    570..665
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1812..1911)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1941..1960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1942..1960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1965..1981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1965..1981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2083..2102)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2094..2903
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2161..2180)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3050..3638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3539..3558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtaatg atgttctcgg gcttcaacgc agactacgag gcgtcatcct cccgctgcag
      721 cagcgcgtcc ccggccgggg atagcctctc ttactaccac tcacccgcag actccttctc
      781 cagcatgggc tcgcctgtca acgcgcagga cttctgcacg gacctggccg tctccagtgc
      841 caacttcatt cccacggtca ctgccatctc gaccagtccg gacctgcagt ggctggtgca
      901 gcccgccctc gtctcctctg tggccccatc gcagaccaga gcccctcacc ctttcggagt
      961 ccccgccccc tccgctgggg cttactccag ggctggcgtt gtgaagacca tgacaggagg
     1021 ccgagcgcag agcattggca ggaggggcaa ggtggaacag ttatctccag aagaagaaga
     1081 gaaaaggaga atccgaaggg aaaggaataa gatggctgca gccaaatgcc gcaaccggag
     1141 gagggagctg actgatacac tccaagcgga gacagaccaa ctagaagatg agaagtctgc
     1201 tttgcagacc gagattgcca acctgctgaa ggagaaggaa aaactagagt tcatcctggc
     1261 agctcaccga cctgcctgca agatccctga tgacctgggc ttcccagaag agatgtctgt
     1321 ggcttccctt gatctgactg ggggcctgcc agaggttgcc accccggagt ctgaggaggc
     1381 cttcaccctg cctctcctca atgaccctga gcccaagccc tcagtggaac ctgtcaagag
     1441 catcagcagc atggagctga agaccgagcc ctttgatgac ttcctgttcc cagcatcatc
     1501 caggcccagt ggctctgaga cagcccgctc cgtgccagac atggacctat ctgggtcctt
     1561 ctatgcagca gactgggagc ctctgcacag tggctccctg gggatggggc ccatggccac
     1621 agagctggag cccctgtgca ctccggtggt cacctgtact cccagctgca ctgcttacac
     1681 gtcttccttc gtcttcacct accccgaggc tgactccttc cccagctgtg cagctgccca
     1741 ccgcaagggc agcagcagca atgagccttc ctctgactcg ctcagctcac ccacgctgct
     1801 ggccctgtga aacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1861 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1921 tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1981 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2041 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2101 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     2161 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     2221 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2281 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2341 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     2401 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2461 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2521 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2581 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2641 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2701 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2761 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2821 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2881 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2941 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     3001 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3661 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
