LOCUS       sequence-178081-        3943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MultiSite Gateway entry clones, second fragment (attL5, attL2) Human
            NR1H2 CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner
            E, Nelson PJ
  TITLE     A vector platform for the rapid and efficient engineering of stable 
            complex transgenes.
  JOURNAL   Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
  PUBMED    27694838
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-3"; volume: "6:34365. doi"; pages: " 
            10.1038/srep34365"
COMMENT     Sequence Label: pENTR221-NR1H2
FEATURES             Location/Qualifiers
     source          1..3943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    570..665
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2055..2154)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2184..2203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2185..2203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2208..2224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2208..2224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2326..2345)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2337..3146
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2404..2423)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3293..3881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3782..3801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtaatg tcctctccta ccacgagttc cctggatacc cccctgcctg gaaatggccc
      721 ccctcagcct ggcgcccctt cttcttcacc cactgtaaag gaggagggtc cggagccgtg
      781 gcccgggggt ccggaccctg atgtcccagg cactgatgag gccagctcag cctgcagcac
      841 agactgggtc atcccagatc ccgaagagga accagagcgc aagcgaaaga agggcccagc
      901 cccgaagatg ctgggccacg agctttgccg tgtctgtggg gacaaggcct ccggcttcca
      961 ctacaacgtg ctcagctgcg aaggctgcaa gggcttcttc cggcgcagtg tggtccgtgg
     1021 tggggccagg cgctatgcct gccggggtgg cggaacctgc cagatggacg ctttcatgcg
     1081 gcgcaagtgc cagcagtgcc ggctgcgcaa gtgcaaggag gcagggatga gggagcagtg
     1141 cgtcctttct gaagaacaga tccggaagaa gaagattcgg aaacagcagc agcaggagtc
     1201 acagtcacag tcgcagtcac ctgtggggcc gcagggcagc agcagctcag cctctgggcc
     1261 tggggcttcc cctggtggat ctgaggcagg cagccagggc tccggggaag gcgagggtgt
     1321 ccagctaaca gcggctcaag aactaatgat ccagcagttg gtggcggccc aactgcagtg
     1381 caacaaacgc tccttctccg accagcccaa agtcacgccc tggcccctgg gcgcagaccc
     1441 ccagtcccga gatgcccgcc agcaacgctt tgcccacttc acggagctgg ccatcatctc
     1501 agtccaggag atcgtggact tcgctaagca agtgcctggt ttcctgcagc tgggccggga
     1561 ggaccagatc gccctcctga aggcatccac tatcgagatc atgctgctag agacagccag
     1621 gcgctacaac cacgagacag agtgtatcac cttcttgaag gacttcacct acagcaagga
     1681 cgacttccac cgtgcaggcc tgcaggtgga gttcatcaac cccatcttcg agttctcgcg
     1741 ggccatgcgg cggctgggcc tggacgacgc tgagtacgcc ctgctcatcg ccatcaacat
     1801 cttctcggcc gaccggccca acgtgcagga gccgggccgc gtggaggcgt tgcagcagcc
     1861 ctacgtggag gcgctgctgt cctacacgcg catcaagagg ccgcaggacc agctgcgctt
     1921 cccgcgcatg ctcatgaagc tggtgagcct gcgcacgctg agctctgtgc actcggagca
     1981 ggtcttcgcc ttgcggctcc aggacaagaa gctgccgcct ctgctgtcgg agatctggga
     2041 cgtccacgag tgaaacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2101 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2161 agctgcaggg cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2221 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2281 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2341 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     2401 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     2461 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     2521 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     2581 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     2641 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     2701 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2761 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2821 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2881 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2941 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     3001 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     3061 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     3121 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     3181 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     3241 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
