LOCUS       sequence-178086-       11706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii
            NCIMB 8052.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren 
            X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
  TITLE     CRISPR-based genome editing and expression control systems in 
            Clostridium acetobutylicum and Clostridium beijerinckii.
  JOURNAL   Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
  PUBMED    27213844
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2016 May 23. doi: 10.1002/biot.201600053."
COMMENT     Sequence Label: pNICKclos2.0
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1488..1522
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1543..1618
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3804..3830,3831..3833,3834..7910)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7927..7944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7927..7943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7936..7958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8152..8171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8271..8293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8331..8349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8417..8521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8522..8590,8591..9382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8740..8759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9553..10141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10042..10061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10295..10312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10429..10450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10465..10470,10471..10488,10489..10495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10503..10519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10508..10530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10527..10543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10527..10543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgataattta cagaaaagaa aattatagaa tttagtatga ttaattatac tcatttatga
       61 atgtttaatt gaatacaaaa aaaaantact tgttatgtat tcaattacgg gttaaaatat
      121 agacaagttg aaaaatttaa taaaaaaata agtcctcagc tcttatatat taagctacca
      181 acttagtata taagccaaaa cttaaatgtg ctaccaacac atcaagccgt tagagaactc
      241 tatctatagc aatatttcaa atgtaccgac atacaagaga aacattaact atatatattc
      301 aatttatgag attatcttaa cagatataaa tgtaaattgc aataagtaag atttagaagt
      361 ttatagcctt tgtgtattgg aagcagtacg caaaggcttt tttatttgat aaaaattaga
      421 agtatattta ttttttcata attaatttat gaaaatgaaa gggggtgagc aaagtgacag
      481 aggaaagcag tatcttatca aataacaagg tattagcaat atcattattg actttagcag
      541 taaacattat gacttttata gtgcttgtag ctaagtagta cgaaaggggg agctttaaaa
      601 agctccttgg aatacataga attcataaat taatttatga aaagaagggc gtatatgaaa
      661 acttgtaaaa attgcaaaga gtttattaaa gatactgaaa tatgcaaaat acattcgttg
      721 atgattcatg ataaaacagt agcaacctat tgcagtaaat acaatgagtc aagatgttta
      781 cataaaggga aagtccaatg tattaattgt tcaaagatga accgatatgg atggtgtgcc
      841 ataaaaatga gatgttttac agaggaagaa cagaaaaaag aacgtacatg cattaaatat
      901 tatgcaagga gctttaaaaa agctcatgta aagaagagta aaaagaaaaa ataatttatt
      961 tattaattta atattgagag tgccgacaca gtatgcacta aaaaatatat ctgtggtgta
     1021 gtgagccgat acaaaaggat agtcactcgc attttcataa tacatcttat gttatgatta
     1081 tgtgtcggtg ggacttcacg acgaaaaccc acaataaaaa aagagttcgg ggtagggtta
     1141 agcatagttg aggcaactaa acaatcaagc taggatatgc agtagcagac cgtaaggtcg
     1201 ttgtttaggt gtgttgtaat acatacgcta ttaagatgta aaaatacgga taccaatgaa
     1261 gggaaaagta taatttttgg atgtagtttg tttgttcatc tatgggcaaa ctacgtccaa
     1321 agccgtttcc aaatctgcta aaaagtatat cctttctaaa atcaaagtca agtatgaaat
     1381 cataaataaa gtttaatttt gaagttatta tgatattatg tttttctatt aaaataaatt
     1441 aagtatatag aatagtttaa taatagtata tacttaatgt gctgcagttg acagctagct
     1501 cagtcctagg tataatacta gtcgagttag acataatagt gagttttaga gctagaaata
     1561 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     1621 ttttgcggcc gcaaaacctg gttctaatgg aattgtttac ttaccatatc ttatgggaga
     1681 aagaacacct catatagacc ctaatgtaaa aggtgcattt ttaggagtat cactaataaa
     1741 taaccatgat gattttgtac gtagtatatt agaaggtgtt agctttagtt tgaaaaactg
     1801 ccttgattta atcgaagaca tgaatgtaaa cattgatgaa ataagagtaa gtggcggtgg
     1861 agcagaaagt gctgtatgga gacaaatatt agctgatatt ttccaatatt cattaactac
     1921 agttaaagca tctgaaggcg gtgcgcttgg agttgcaata ttagcagggg ttggagcagg
     1981 aatctatgat tctgtagaag atgcttgtga tagaatcgta aaaggaaaca agaaagtttc
     2041 tccaaatgaa gaattaaaag aagtatactc aaaagtatat gaaacatata attcagctta
     2101 tccaagaata aaagatattt aaatatatta aagatagaaa tcaatcatct tagttaagta
     2161 attgtataat taattaaggg tgtgtagtag tatcgaattt tagcaaaaaa atcatatagt
     2221 tttttaaata ctttaacaaa aataataata gtgatattat ttaagaaaaa gctactatta
     2281 ctgataatag tcgagaggag tattttaaag tttgatagaa gtaaatcaca gtaaaataaa
     2341 agaaactaac agaaaaaaga taatagcatt attattaaag aaaaatgaga tcaccaaatt
     2401 agatatatct agaatattag atataagtat aacaacagtt tctacaaata ttacagaatt
     2461 gaaaagcgaa ggtatnnnnn nnnatgtaag atcgttggaa tctacaggag gaagaaaagc
     2521 aattgctatt aagttgaatg aaaactgtag atattcaatt ggagtcgcac taacaccgaa
     2581 tcatataaag atatcattag taaatataaa gaaaaaagtt attgaaagta tgctgcagtg
     2641 gaatagagaa cataataaat ttacttaatg aaaatataga tttgttaatg aagaaatata
     2701 atttatgctc tgcaaattta ttaggaatag ggatttcatt acctggaacc gtagatttta
     2761 aagaaggcat aataaagtat tcatatttgt taggcgccaa agattttaat ttaaaggaaa
     2821 agttcgaata cttagacatt ccagtatatg tagataatga agcaaattta tctgcttact
     2881 acgagttttt aaataaaaga gatatactaa gaaatctatt atatgtatca ataacagaag
     2941 gtttaggcct tggtataata ataaatggaa aaatttacag aggtgacaat aactcatcag
     3001 gagaattagg tcatactaag atagcaattg atggaaaaaa atgtaagtgt ggagctagag
     3061 gctgtttaga agcatacaca tctatgaatt ctctgattga tagttataat gaagctaata
     3121 gttcaaatat tagtgatatt gatgaatttg aagaaagata taatcaaaat gacaaagatg
     3181 ctcatgatgt attaaatgat tatttaaaaa cattgtcttt aggtctatcg aatttggtta
     3241 tgatatttga tcctagcagc atagtaatag gtggagatat aaataattta cttaatgata
     3301 agatagatat gctgaaaaaa gaggtttata aagataattt atttacaaat gaaaataatt
     3361 gcagtataag tattgcaagt tttaaagaat cttatctact aggagctgct atgatgccta
     3421 tagaagaatt cttagaaatt aaataatgga gtggtgaaag ctgctctatt aatttaaaaa
     3481 cttattaaat aaatttttaa aatatatttt taaagttatg taagcatagc tatattaagg
     3541 ggggtcttaa tctttgattg gaactccaga ttaagacaag ccttttatat aaaactaaat
     3601 atctatttat ttcatattag atttatgttc ttgattttct cgagttttta acaaaatata
     3661 ttgataaaaa taataatagt gggtataatt aagttgttag agaaaacgta taaattaggg
     3721 ataaactatg gaacttatga aatagattga aatggtttat ctgttacccc gtatcaaaat
     3781 ttaggaggtt agttagaccc gggatggata agaaatactc aataggctta gctatcggca
     3841 caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca
     3901 aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat
     3961 ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata
     4021 cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag
     4081 tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc
     4141 atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc
     4201 caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct
     4261 taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag
     4321 atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca
     4381 atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt
     4441 ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga
     4501 agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta
     4561 aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg
     4621 atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag
     4681 ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa
     4741 ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga
     4801 ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg
     4861 atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt
     4921 ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac
     4981 taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc
     5041 aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt
     5101 taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg
     5161 gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa
     5221 ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg
     5281 aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt
     5341 tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag
     5401 gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct
     5461 tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag
     5521 aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta
     5581 cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg
     5641 aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg
     5701 aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac
     5761 gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata
     5821 agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt
     5881 ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag
     5941 tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta
     6001 ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc
     6061 ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg
     6121 gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa
     6181 gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc
     6241 tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt
     6301 taagtgatta tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag
     6361 acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg
     6421 aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca
     6481 ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata
     6541 aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac
     6601 aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg
     6661 ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct
     6721 ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg
     6781 ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt
     6841 ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa
     6901 ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg
     6961 caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg
     7021 tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca
     7081 atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa
     7141 aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg
     7201 gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga
     7261 aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt
     7321 cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag
     7381 acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga
     7441 tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg
     7501 tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg
     7561 aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa
     7621 tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg
     7681 catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat
     7741 ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc
     7801 gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca
     7861 ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactga gcggccgcgg
     7921 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7981 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8041 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     8101 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     8161 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     8221 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8281 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     8341 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8521 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8581 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8641 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8701 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8761 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8821 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8881 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8941 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9001 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9061 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9121 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9181 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9241 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9301 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9361 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9421 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9481 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9661 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10201 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10261 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10321 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10381 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10441 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10501 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
    10561 ttggctaaca cacacgccat tccaaccaat agttttctcg gcataaagcc atgctctgac
    10621 gcttaaatgc actaatgcct taaaaaaaca ttaaagtcta acacactaga cttatttact
    10681 tcgtaattaa gtcgttaaac cgtgtgctct acgaccaaaa gtataaaacc tttaagaact
    10741 ttcttttttc ttgtaaaaaa agaaactaga taaatctctc atatctttta ttcaataatc
    10801 gcatcagatt gcagtataaa tttaacgatc actcatcatg ttcatattta tcagagctcg
    10861 tgctataatt atactaattt tataaggagg aaaaaataaa gagggttata atgaacgaga
    10921 aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat aaaataatga
    10981 caaatataag attaaatgaa catgataata tctttgaaat cggctcagga aaagggcatt
    11041 ttacccttga attagtacag aggtgtaatt tcgtaactgc cattgaaata gaccataaat
    11101 tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt ttaaacaagg
    11161 atatattgca gtttaaattt cctaaaaacc aatcctataa aatatttggt aatatacctt
    11221 ataacataag tacggatata atacgcaaaa ttgtttttga tagtatagct gatgagattt
    11281 atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc tcattggcat
    11341 tatttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga gaatattttc
    11401 atcctaaacc taaagtgaat agctcactta tcagattaaa tagaaaaaaa tcaagaatat
    11461 cacacaaaga taaacagaag tataattatt tcgttatgaa atgggttaac aaagaataca
    11521 agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga attgacgatt
    11581 taaacaatat tagctttgaa caattcttat ctcttttcaa tagctataaa ttatttaata
    11641 agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgta cctatttttt
    11701 gtgaat
//
