LOCUS       sequence-178103-        9776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Rubens JR, Selvaggio G, Lu TK
  TITLE     Synthetic mixed-signal computation in living cells.
  JOURNAL   Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
  PUBMED    27255669
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-3"; volume: "7:11658. doi"; pages: " 
            10.1038/ncomms11658"
COMMENT     Sequence Label: 
            pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(7..93)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(129..752)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     660..679
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(951..1037)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             2576..2587
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(2597..2622,2623..2628,2629..2645,2646..2651,
                     2652..2670))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2627..2645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2652..2670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3151..4965
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     terminator      5073..5159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6302..6388
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6933..7883)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      7931..8153
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(8651..8745)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(8775..9566,9567..9635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9398..9417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9636..9740)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
       61 ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagcacgcgt accatgggat
      121 cccccgggtt aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
      181 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt
      241 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
      301 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat
      361 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
      421 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
      481 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
      541 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc
      601 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
      661 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg
      721 ttaatcactt tacttttatc taatctagac atggtaccgg tttcctgtgt gaagaattca
      781 aagttaaaca aaattatttg tagagggaaa ccgttgtggt ctccctgaat atagcctacg
      841 agccttatgc atgcccgtaa agttatccag caaccactca tagacctagg gcagcagata
      901 gggacgacgt ggtgttagct gtggctagca tctcgagtct agggcggcgg atttgtccta
      961 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     1021 gcctttcgtt ttatttgatg cctctagcac gcgtaccatg ggatcccccg ggctaaactg
     1081 ctgcagcgta gttttcgtcg tttgctgcag gcctagcgag ttggaattta aatatgatat
     1141 ctacattatc agcagtaaca tcaacctttg atacaaggtt gttgacgatt ttctttttat
     1201 tatcatatga tagttcatta atcggaattg agcccaactg agttttaact aactcaaaaa
     1261 catcagtaga gtcattaaat ttattttcgc taatcttagc tttaagcagc tttttctcag
     1321 cctgaaggga atcagtacga tctttcaact catccatagt gataaaatca tttaggtaca
     1381 aatcagagtt cttttgtatt tttttatcga tctgtgaaat ttgcttttta aatgacgaag
     1441 tatcaagaat aggttggttg ttgccattga taattttcaa taaggagtca ttattttctt
     1501 gaaatccaat caggttgtca ataacagtat tttctaaatt acttaaatca taagttcctg
     1561 aatcacactt tttattgtca ttatatactg taattccttt tgtttttcga ggaaatctat
     1621 ttgcacagtg atatttcata gtgcggcttc catcttttct tttgtggcca agaacaattt
     1681 ttaaaggtgc tccacagtaa ccgcaccttg ccatccctga cagcatatat ttagcttgga
     1741 aaggtctagg gttgttattt ctttcataag tctgctgttg tctttcttct agctcttttt
     1801 gaacttttaa ataagtctca taagggataa ttggtttgtg cataccttca aataggctgt
     1861 ccttaaattt gatataacca cagtaaactg gattatcaag tgtttgtctt agggtacgat
     1921 aagaccacgg tatatcttta ccgatgtgtc cagattcatt gagtttatct cttaattttg
     1981 taagtgatat tcctgataaa taatcagtga atatttgttc aactattgta gcttgtaaag
     2041 gaacaatttc taatatacct gtctttctgt tgtggtaata cccaaaagct gtcttagtcc
     2101 acatcataga cttaccagat ttcgctcgcc ctagtttacc catagtcatg cgttctttta
     2161 tattctctct ttcaaactca ttaattgcag aaagaatagt gagaaacaag ctacccatag
     2221 cagaagaagt atcaatactt tcattaagcg agataaagtc tattttattt tttgtgaaca
     2281 catccttaac aagataaaga gtatctctta cactacgtga aaggcggtct agcttatata
     2341 caagaactgt atcaaaagct ttattctcga tatcgttgat taatctttgc attgctgggc
     2401 gttcaagttt ggcccctgaa aaaccagcat cagtataagt atcagatact tgccacccca
     2461 ttgcttcagc atattttgtt aaacggtcaa tttgctcatc aattgagaag ccttcctctg
     2521 cttggttagt agtggatact cgtgtataga ttgctacttt cttagtcatg gtacctttct
     2581 cctctttaat gaattcggtc agtgcgtcct gctgatgtgc tcagtatctc tatcactgat
     2641 agggatgtca atctctatca ctgataggga gacgtcaggt ggcacttttc ggggaaatgt
     2701 gcgcggaacc cctatttgtt tatttttcta aatacattca rayaaatccg ccgccctaga
     2761 gacgtcgacg tctgtggctt ttatgaaaat cacacagtga tcacaaattt taaacagagc
     2821 acaaaatgct gcctcgaaat gagggcggga aaataaggtt atcagccttg ttttctccct
     2881 cattacttga aggatatgaa gctaaaaccc ttttttataa agcatttgtc cgaattcgga
     2941 cataatcaaa aaagcttaat taagatcaat ttgatctaca tctctttaac caacaatatg
     3001 taagatctca actatcgcat ccgtggatta attcaattat aacttctctc taacgctgtg
     3061 tatcgtaacg gtaacactgt agaggggagc acattgatgc gaattctcac acaggaaacc
     3121 ggtaccatga cacaaggggt tgtgaccggg gtggacacgt acgcgggtgc ttacgaccgt
     3181 cagtcgcgcg agcgcgaaaa ttcgagcgca gcaagcccag cgacacagcg tagcgccaac
     3241 gaagacaagg cggccgacct tcagcgcgaa gtcgagcgcg acgggggccg gttcaggttc
     3301 gtcgggcatt tcagcgaagc gccgggcacg tcggcgttcg ggacggcgga gcgcccggag
     3361 ttcgaacgca tcctgaacga atgccgcgcc gggcggctca acatgatcat tgtctatgac
     3421 gtgtcgcgct tctcgcgcct gaaggtcatg gacgcgattc cgattgtctc ggaattgctc
     3481 gccctgggcg tgacgattgt ttccactcag gaaggcgtct tccggcaggg aaacgtcatg
     3541 gacctgattc acctgattat gcggctcgac gcgtcgcaca aagaatcttc gctgaagtcg
     3601 gcgaagattc tcgacacgaa gaaccttcag cgcgaattgg gcgggtacgt cggcgggaag
     3661 gcgccttacg gcttcgagct tgtttcggag acgaaggaga tcacgcgcaa cggccgaatg
     3721 gtcaatgtcg tcatcaacaa gcttgcgcac tcgaccactc cccttaccgg acccttcgag
     3781 ttcgagcccg acgtaatccg gtggtggtgg cgtgagatca agacgcacaa acaccttccc
     3841 ttcaagccgg gcagtcaagc cgccattcac ccgggcagca tcacggggct ttgtaagcgc
     3901 atggacgctg acgccgtgcc gacccggggc gagacgattg ggaagaagac cgcttcaagc
     3961 gcctgggacc cggcaaccgt tatgcgaatc cttcgggacc cgcgtattgc gggcttcgcc
     4021 gctgaggtga tctacaagaa gaagccggac ggcacgccga ccacgaagat tgagggttac
     4081 cgcattcagc gcgacccgat cacgctccgg ccggtcgagc ttgattgcgg accgatcatc
     4141 gagcccgctg agtggtatga gcttcaggcg tggttggacg gcagggggcg cggcaagggg
     4201 ctttcccggg ggcaagccat tctgtccgcc atggacaagc tgtactgcga gtgtggcgcc
     4261 gtcatgactt cgaagcgcgg ggaagaatcg atcaaggact cttaccgctg ccgtcgccgg
     4321 aaggtggtcg acccgtccgc acctgggcag cacgaaggca cgtgcaacgt cagcatggcg
     4381 gcactcgaca agttcgttgc ggaacgcatc ttcaacaaga tcaggcacgc cgaaggcgac
     4441 gaagagacgt tggcgcttct gtgggaagcc gcccgacgct tcggcaagct cactgaggcg
     4501 cctgagaaga gcggcgaacg ggcgaacctt gttgcggagc gcgccgacgc cctgaacgcc
     4561 cttgaagagc tgtacgaaga ccgcgcggca ggcgcgtacg acggacccgt tggcaggaag
     4621 cacttccgga agcaacaggc agcgctgacg ctccggcagc aaggggcgga agagcggctt
     4681 gccgaacttg aagccgccga agccccgaag cttccccttg accaatggtt ccccgaagac
     4741 gccgacgctg acccgaccgg ccctaagtcg tggtgggggc gcgcgtcagt agacgacaag
     4801 cgcgtgttcg tcgggctctt cgtagacaag atcgttgtca cgaagtcgac tacgggcagg
     4861 gggcagggaa cgcccatcga gaagcgcgct tcgatcacgt gggcgaagcc gccgaccgac
     4921 gacgacgaag acgacgccca ggacggcacg gaagacgtag cggcgaggcc tgcagcaaac
     4981 gacgaaaact acgctgcagc agtttagaca catggcatgg atgaactata caaataaccc
     5041 gggggatccc atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag
     5101 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     5161 cgccgcccta gacctagcac agctaacacc acgtcgtccc tatctgctgc cctaggtcta
     5221 tgagtggttg ctggataact ttacgggcat gcataaggct cgtataatat attcagggag
     5281 accacaacgg tttccctcta caaataattt tgtttaactt tgaattcttc acacaggaaa
     5341 ccggtaccat gaatattcgt gatcttgagt acctggtggc attggctgaa caccgccatt
     5401 ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg caaattcgta
     5461 agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa gtgttgttca
     5521 cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag gtgaaagtcc
     5581 ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcac attggtttga
     5641 ttcccacagt tggaccgtac ctgctaccgc atattatccc tatgctgcac cagacctttc
     5701 caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg caactggaca
     5761 gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag
     5821 tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg tgggcgaacc
     5881 gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg gaagatggtc
     5941 actgtttgcg cgatcaggca atgggtttct gttttgaagc cggggcggat gaagatacac
     6001 acttccgcgc gaccagcctg gaaactctgc gcaacatggt ggcggcaggt agcgggatca
     6061 ctttactgcc agcgctggct gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc
     6121 cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc
     6181 tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc aagaatggat ggccatttcg
     6241 ataaagtttt aaaacaggcg gtttaacccg ggggatccca tggtacgcgt gctagaggca
     6301 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     6361 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagggcc tagggtacgg
     6421 gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag aatcgcagat
     6481 ccggcttcag ccggtttgcc ggctgaaagc gctatttctt ccagaattgc catgattttt
     6541 tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg ataagcagca
     6601 tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt
     6661 caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta ggtgttacat
     6721 gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttgaat gcaccaaaaa
     6781 ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg catatggaca
     6841 gttttccctt tgatatgtaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg
     6901 cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg
     6961 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatac
     7021 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     7081 aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt aggaatctga tgtaatggtt
     7141 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     7201 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     7261 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     7321 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     7381 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     7441 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     7501 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     7561 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     7621 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     7681 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     7741 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     7801 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     7861 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     7921 caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc
     7981 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     8041 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     8101 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     8161 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     8221 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     8281 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     8341 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     8401 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     8461 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     8521 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     8581 atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tccctagtac
     8641 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     8701 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa
     8761 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8821 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8881 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8941 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9001 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9061 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9121 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9181 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9241 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9301 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9361 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9421 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9481 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9541 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9601 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9661 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9721 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctccat
//
