LOCUS       sequence-178105-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Rubens JR, Selvaggio G, Lu TK
  TITLE     Synthetic mixed-signal computation in living cells.
  JOURNAL   Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
  PUBMED    27255669
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-3"; volume: "7:11658. doi"; pages: " 
            10.1038/ncomms11658"
COMMENT     Sequence Label: pZS1katGp-RBS33-TP901-proD-oxyR
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1914..2000
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3143..3229
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3774..4724)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4772..4994
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5492..5586)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5616..6407,6408..6476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6239..6258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6477..6581)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtggctttt atgaaaatca cacagtgatc acaaatttta aacagagcac aaaatgctgc
       61 ctcgaaatga gggcgggaaa ataaggttat cagccttgtt ttctccctca ttacttgaag
      121 gatatgaagc taaaaccctt ttttataaag catttgtccg aattcggaca taatcaaaaa
      181 agcttaatta agatcaattt gatctacatc tctttaacca acaatatgta agatctcaac
      241 tatcgcatcc gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt
      301 aacactgtag aggggagcac attgatgcga attctcacac aggacggtac catgactaag
      361 aaagtagcaa tctatacacg agtatccact actaaccaag cagaggaagg cttctcaatt
      421 gatgagcaaa ttgaccgttt aacaaaatat gctgaagcaa tggggtggca agtatctgat
      481 acttatactg atgctggttt ttcaggggcc aaacttgaac gcccagcaat gcaaagatta
      541 atcaacgata tcgagaataa agcttttgat acagttcttg tatataagct agaccgcctt
      601 tcacgtagtg taagagatac tctttatctt gttaaggatg tgttcacaaa aaataaaata
      661 gactttatct cgcttaatga aagtattgat acttcttctg ctatgggtag cttgtttctc
      721 actattcttt ctgcaattaa tgagtttgaa agagagaata taaaagaacg catgactatg
      781 ggtaaactag ggcgagcgaa atctggtaag tctatgatgt ggactaagac agcttttggg
      841 tattaccaca acagaaagac aggtatatta gaaattgttc ctttacaagc tacaatagtt
      901 gaacaaatat tcactgatta tttatcagga atatcactta caaaattaag agataaactc
      961 aatgaatctg gacacatcgg taaagatata ccgtggtctt atcgtaccct aagacaaaca
     1021 cttgataatc cagtttactg tggttatatc aaatttaagg acagcctatt tgaaggtatg
     1081 cacaaaccaa ttatccctta tgagacttat ttaaaagttc aaaaagagct agaagaaaga
     1141 caacagcaga cttatgaaag aaataacaac cctagacctt tccaagctaa atatatgctg
     1201 tcagggatgg caaggtgcgg ttactgtgga gcacctttaa aaattgttct tggccacaaa
     1261 agaaaagatg gaagccgcac tatgaaatat cactgtgcaa atagatttcc tcgaaaaaca
     1321 aaaggaatta cagtatataa tgacaataaa aagtgtgatt caggaactta tgatttaagt
     1381 aatttagaaa atactgttat tgacaacctg attggatttc aagaaaataa tgactcctta
     1441 ttgaaaatta tcaatggcaa caaccaacct attcttgata cttcgtcatt taaaaagcaa
     1501 atttcacaga tcgataaaaa aatacaaaag aactctgatt tgtacctaaa tgattttatc
     1561 actatggatg agttgaaaga tcgtactgat tcccttcagg ctgagaaaaa gctgcttaaa
     1621 gctaagatta gcgaaaataa atttaatgac tctactgatg tttttgagtt agttaaaact
     1681 cagttgggct caattccgat taatgaacta tcatatgata ataaaaagaa aatcgtcaac
     1741 aaccttgtat caaaggttga tgttactgct gataatgtag atatcatatt taaattccaa
     1801 ctcgctaggc ctgcagcaaa cgacgaaaac tacgctgcag cagtttagac acatggcatg
     1861 gatgaactat acaaataacc cgggggatcc catggtacgc gtgctagagg catcaaataa
     1921 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1981 ctctcctgag taggacaaat ccgccgccct agacctagca cagctaacac cacgtcgtcc
     2041 ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca tgcataaggc
     2101 tcgtataata tattcaggga gaccacaacg gtttccctct acaaataatt ttgtttaact
     2161 ttgaattctt cacacaggaa accggtacca tgaatattcg tgatcttgag tacctggtgg
     2221 cattggctga acaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga
     2281 cgcttagcgg gcaaattcgt aagctggaag atgagctggg cgtgatgttg ctggagcgga
     2341 ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtggatcag gcgcgtaccg
     2401 tgctgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag acgatgtccg
     2461 gaccgctgca cattggtttg attcccacag ttggaccgta cctgctaccg catattatcc
     2521 ctatgctgca ccagaccttt ccaaagctgg aaatgtatct gcatgaagca cagacccacc
     2581 agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag
     2641 agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg
     2701 aagatcaccc gtgggcgaac cgcgaatgcg taccgatggc cgatctggca ggggaaaaac
     2761 tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgttttgaag
     2821 ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg cgcaacatgg
     2881 tggcggcagg tagcgggatc actttactgc cagcgctggc tgtgccgccg gagcgcaaac
     2941 gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact attggcctgg
     3001 tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgcg
     3061 caagaatgga tggccatttc gataaagttt taaaacaggc ggtttaaccc gggggatccc
     3121 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3181 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     3241 gacctagggc ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     3301 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     3361 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     3421 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     3481 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     3541 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     3601 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     3661 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     3721 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     3781 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     3841 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     3901 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     3961 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     4021 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     4081 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     4141 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     4201 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     4261 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     4321 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     4381 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     4441 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     4501 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     4561 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     4621 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     4681 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     4741 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     4801 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     4861 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     4921 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     4981 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     5041 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     5101 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     5161 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     5221 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     5281 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     5341 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     5401 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     5461 ccgtcttact gtccctagta ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     5521 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     5581 ggagtccaag cgagctcgta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6601 ccacctgacg tc
//
