LOCUS       sequence-178106-       10587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Rubens JR, Selvaggio G, Lu TK
  TITLE     Synthetic mixed-signal computation in living cells.
  JOURNAL   Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
  PUBMED    27255669
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-3"; volume: "7:11658. doi"; pages: " 
            10.1038/ncomms11658"
COMMENT     Sequence Label: ADC BAC Reporter
FEATURES             Location/Qualifiers
     source          1..10587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    166..214
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     terminator      complement(234..320)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(423..443)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     RBS             1102..1110
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(1146..1195)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    1204..1261
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             complement(1829..2800)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2800..3975)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    4301..4551
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4554..5309)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      5400..5619
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      5687..6218
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5695..6309
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        7167..7269
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7270..7929
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7336..7355)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     7972..7990
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8235..8321)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8354..9067)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Mena et al., 2006)"
                     /label=Azurite BFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FRTAAGITHGMDELYK"
     misc_RNA        complement(9116..9165)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    9453..9486
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      complement(9512..9598)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             10352..10360
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(10399..10448)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 ggctgaactc gagcacagct aacaccacgt cgtccctatc tgctgcccta ggtctatgag
       61 tggttgctgg ataactttac gggcatgcat aaggctcgta taatatattc agggagacca
      121 caacggtttc cctctacaaa taattttgtt taacttttta attaacggcc ggcttgtcga
      181 cgacggcggt ctccgtcgtc aggatcatcc gggcatcgat tctagggcgg cggatttgtc
      241 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      301 tgagcctttc gttttatttg atgcctctag cacgcgtacc atgggatccc ccgggctgca
      361 ggaattcgat atcaagcttt tatttgtaga gatcatccat gccatgtgta atcccagcag
      421 ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg
      481 cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
      541 tattttgttg ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt
      601 tgaagttaac tttgattcca ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt
      661 tatagttgta ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt
      721 ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt
      781 agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac
      841 tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct cgcaaagcat tgaacaccat
      901 aaccgaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa
      961 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt
     1021 tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt
     1081 cttctccttt actcatctta aacctcctta cctcgtaaac tattaaacaa aattatttgt
     1141 agaggctgtt tcgtcctcac ggactcatca gaccggaaag cacatccggt gacagctgtg
     1201 cacgtcgggg tttgtaccgt acaccactga gaccgcggtg gttgaccaga caaaccacga
     1261 cacatgtcaa tacttgccct tgacaggcat tgatggaatc gtagtctcac gctgatagtc
     1321 tgatcgacaa tacaagtggg accgtggtcc cagaccgata atcagaccga caacacgagt
     1381 gggatcgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccaga
     1441 ctaataatca gaccgacgat acgagtggga ccgtggttcc agactaataa tcagaccgac
     1501 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccat
     1561 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat
     1621 cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag
     1681 tgggaccgtg gtcccagtct gattatcaga ccgacgatac aagtggaaca gtgggcccag
     1741 agagaatatt caggccagtt atgctttctg gcctgtaaca aaggacatta agtaaagaca
     1801 gataaacgta gactaaaacg tggtcgcatc agggtgctgg cttttcaagt tccttaagaa
     1861 tggcctcaat tttctctata cactcagttg gaacacggga cctgtccagg ttaagcacca
     1921 ttttatcgcc cttatacaat actgtcgctc caggagcaaa ctgatgtcgt gagcttaaac
     1981 tagttcttga tgcagatgac gttttaagca cagaagttaa aagagtgata acttcttcag
     2041 cttcaaatat caccccagct tttttctgct catgaaggtt agatgcctgc tgcttaagta
     2101 attcctcttt atctgtaaag gctttttgaa gtgcatcacc tgaccgggca gatagttcac
     2161 cggggtgaga aaaaagagca acaactgatt taggcaattt ggcggtgttg atacagcggg
     2221 taataatctt acgtgaaata ttttccgcat cagccagcgc agaaatattt ccagcaaatt
     2281 cattctgcaa tcggcttgca taacgctgac cacgttcata agcacttgtt gggcgataat
     2341 cgttacccaa tctggataat gcagccatct gctcatcatc cagctcgcca accagaacac
     2401 gataatcact ttcggtaagt gcagcagctt tacgacggcg actcccatcg gcaatttcta
     2461 tgacaccaga tactcttcga ccgaacgccg gtgtctgttg accagtcagt agaaaagaag
     2521 ggatgagatc atccagtgcg tcctcagtaa gcagctcctg gtcacgttca ttacctgacc
     2581 atacccgaga ggtcttctca acactatcac cccggagcac ttcaagagta aacttcacat
     2641 cccgaccaca tacaggcaaa gtaatggcat taccgcgagc cattactcct acgcgcgcaa
     2701 ttaacgaatc caccatcggg gcagctggtg tcgataacga agtatcttca accggttgag
     2761 tattgagcgt atgttttgga ataacaggcg cacgcttcat tatctaatct cccagcgtgg
     2821 tttaatcaga cgatcgaaaa tttcattgca gacaggttcc caaatagaaa gagcatttct
     2881 ccaggcacca gttgaagagc gttgatcaat ggcctgttca aaaacagttc tcatccggat
     2941 ctgaccttta ccaacttcat ccgtttcacg tacaacattt tttagaacca tgcttcccca
     3001 ggcatcccga atttgctcct ccatccacgg ggactgagag ccattactat tgctgtattt
     3061 ggtaagcaaa atacgtacat caggctcgaa ccctttaaga tcaacgttct tgagcagatc
     3121 acgaagcata tcgaaaaact gcagtgcgga ggtgtagtca aacaactcag caggcgtggg
     3181 aacaatcagc acatcagcag cacatacgac attaatcgtg ccgataccca ggttaggcgc
     3241 gctgtcaata actatgacat catagtcatg agcaacagtt tcaatggcca gtcggagcat
     3301 caggtgtgga tcggtgggca gtttaccttc atcaaatttg cccattaact cagtttcaat
     3361 acggtgcaga gccagacagg aaggaataat gtcaagcccc ggccagcaag tgggctttat
     3421 tgcataagtg acatcgtcct tttccccaag atagaaaggc aggagagtgt cttctgcatg
     3481 aatatgaaga tctggtaccc atccgtgata cattgaggct gttccctggg ggtcgttacc
     3541 ttccacgagc aaaacacgta gccccttcag agccagatcc tgagcaagat gaacagaaac
     3601 tgaggttttg taaacgccac ctttatgggc agcaaccccg atcaccggtg gaaatacgtc
     3661 ttcagcacgt cgcaatcgcg taccaaacac atcacgcata tgattaattt gttcaattgt
     3721 ataaccaaca cgttgctcaa cccgtcctcg aatttccata tccgggtgcg gtagtcgccc
     3781 tgctttctcg gcatctctga tagcctgaga agaaacccca actaaatccg ctgcttcacc
     3841 tattctccag cgccgggtta ttttcctcgc ttccgggctg tcatcattaa actgtgcaat
     3901 ggcgatagcc ttcgtcattt catgaccagc gtttatgcac tggttaagtg tttccatgag
     3961 tttcattctg aacatccttt aatcattgct ttgcgttttt ttattaaatc ttgcaattta
     4021 ctgcaaagca acaacaaaat cgcaaagtca tcaaaaaacc gcaaagttgt ttaaaataag
     4081 agcaacacta caaaaggaga taagaagagc acatacctca gtcacttatt atcactagcg
     4141 ctcgccgcag ccgtgtaacc gagcatagcg agcgaactgg cgaggaagca aagaagaact
     4201 gttctgtcag atagctctta cgctcagcgc aagaagaaat atccaccgtg ggaaaaactc
     4261 caggtagagg tacacacgcg gatagccaat tcagagtaat aaactgtgat aatcaaccct
     4321 catcaatgat gacgaactaa cccccgatat caagtcacat gacgaaggga aagagaagga
     4381 aatcaactgt gacaaactgc cctcaaattt ggcttcctta aaaattacag ttcaaaaagt
     4441 atgagaaaat ccatgcaggc tgaaggaaac agcaaaactg tgacaaatta ccctcagtag
     4501 gtcagaacaa atgtgacgaa ccaccctcaa atctgtgaca gataaccctc agactatcct
     4561 gtcgtcatgg aagtgatatc gcggaaggaa aatacgatat gagtcgtctg gcggcctttc
     4621 tttttctcaa tgtatgagag gcgcattgga gttctgctgt tgatctcatt aacacagacc
     4681 tgcaggaagc ggcggcggaa gtcaggcata cgctggtaac tttgaggcag ctggtaacgc
     4741 tctatgatcc agtcgatttt cagagagacg atgcctgagc catccggctt acgatactga
     4801 cacagggatt cgtataaacg catggcatac ggattggtga tttcttttgt ttcactaagc
     4861 cgaaactgcg taaaccggtt ctgtaacccg ataaagaagg gaatgagata tgggttgata
     4921 tgtacactgt aaagccctct ggatggactg tgcgcacgtt tgataaacca aggaaaagat
     4981 tcatagcctt tttcatcgcc ggcatcctct tcagggcgat aaaaaaccac ttccttcccc
     5041 gcgaaactct tcaatgcctg ccgtatatcc ttactggctt ccgcagaggt caatccgaat
     5101 atttcagcat atttagcaac atggatctcg cagataccgt catgttcctg tagggtgcca
     5161 tcagattttc tgatctggtc aacgaacaga tacagcatac gtttttgatc ccgggagaga
     5221 ctatatgccg cctcagtgag gtcgtttgac tggacgattc gcgggctatt tttacgtttc
     5281 ttgtgattga taaccgctgt ttccgccatg acagatccat gtgaagtgtg acaagttttt
     5341 agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg aaaaaacagc
     5401 ttcttctgag ggcaatttgt cacagggtta agggcaattt gtcacagaca ggactgtcat
     5461 ttgagggtga tttgtcacac tgaaagggca atttgtcaca acaccttctc tagaaccagc
     5521 atggataaag gcctacaagg cgctctaaaa aagaagatct aaaaactata aaaaaaataa
     5581 ttataaaaat atccccgtgg ataagtggat aaccccaagg gaagtttttt caggcatcgt
     5641 gtgtaagcag aatatataag tgctgttccc tggtgcttcc tcgctcactc gaccgggagg
     5701 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
     5761 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
     5821 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
     5881 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
     5941 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
     6001 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
     6061 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
     6121 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
     6181 gccaagtttt ccgcgaggta tccacaacgc cggcggccgg ccgcggtgtc tcgcacacgg
     6241 cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag
     6301 ggcaaccagc cgagggcttc gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa
     6361 aggacagacc acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat
     6421 ccgctccact tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa
     6481 atcgttttcg ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca
     6541 caggctcctg agattaataa tgcggatctc tacgataatg ggagattttc ccgactgttt
     6601 cgttcgcttc tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc
     6661 cactcagttc cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca
     6721 gcatcgcaac cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat
     6781 ccagcgcaaa aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc
     6841 tttgcagaac tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga
     6901 acgtatgtgg tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg
     6961 cctccccaat ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg
     7021 tccttcctgt gcgacggtta cgccgctcca tgagcttatc gcgaataaat acctgtgacg
     7081 gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg
     7141 gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
     7201 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     7261 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     7321 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     7381 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     7441 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
     7501 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     7561 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     7621 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     7681 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     7741 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     7801 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     7861 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     7921 ggggcgtaag acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7981 atcacgaggc cctttcgtct tcacctcgag cacagctaac accacgtcgt ccctatctgc
     8041 tgccctaggt ctatgagtgg ttgctggata actttacggg catgcataag gctcgtataa
     8101 tatattcagg gagaccacaa cggtttccct ctacaaataa ttttgtttaa ctttttaatt
     8161 aaatgccaac acaattaaca tctcaatcaa ggtaaatgct ttttgctttt tttgcatcga
     8221 ttctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     8281 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccacgc gtaccatggg
     8341 atcccccggg ttatttgtac aattcatcca taccatgggt aataccagca gcagtcctaa
     8401 attctaacag gaccatgtgg tctctctttt cgtttggatc tttggataag gctgattggg
     8461 tggataagta atggttgtct ggtaacaaga ctggaccatc accaattgga gtattttgtt
     8521 gataatggtc agctaattga acagaaccat cttcaatgtt gtgtctaatt ttgaagttca
     8581 ctttgatacc attcttttgt ttgtcagcca tgatgtatat attgtgagag ttgaagttgt
     8641 attccaattt gtgacctaaa atgttaccat cttctttaaa atcaatacct tttaattcga
     8701 ttctattaac taaggtatca ccttcaaact tgacttcagc tctggtcttg tagttaccgt
     8761 catctttgaa aaaaatagtt ctttcttgaa cataaccttc tggcatggca gacttgaaaa
     8821 agtcatgttg tttcatatga tctgggtatc tagaaaaaca ttgaacacca tggctcaaag
     8881 tagttactaa ggttggccat ggaactggca atttaccagt agtacaaata aattttaagg
     8941 tcaatttacc gtacgtagca tcaccttcac cttcaccgga gacagaaaat ttgtgaccat
     9001 taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
     9061 tagacatttt taacctcctc cctacgtact cattaaacaa aattatttgt agaggctgtt
     9121 tcgtcctcac ggactcatca gaccggaaag cacatccggt gacagctgtg tttaaaggag
     9181 ttttttagtt accttaattg aaataaacga aataaaaact cgccgagcac cgcctttggt
     9241 cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttccc tcgagcacag
     9301 ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt
     9361 acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt tccctctaca
     9421 aataattttg tttaactttt taattaatgc gggtgccagg gcgtgccctt gggctccccg
     9481 ggcgcgtact ccatcgattc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     9541 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     9601 gcctctagca cgcgtaccat gggatccccc gggttagttc agtttgtggc ccagtttgga
     9661 aggcagatcg caatagcgtg ccactgccac ttcatgctgt tcgacatagg tttctttgtc
     9721 cgcttctttg atacgttcca gacggcggtc gacataatat acgcccggca ttttcaggtt
     9781 tttagccggt tttttgctgc ggtaagtagt tttcaggtta cagatcaggt ggccgccacc
     9841 aaccagtttc agcgccatat cagaacggcc ttccaggccg ccgtcagccg ggtacagcat
     9901 ttcggtggat gcttcccagc ccagcgtttt tttctgcata accgggccgt tgctcgggaa
     9961 attaacacca cgaattttaa cgttatagat cagacagcca tcctgcagag acgtgtcctg
    10021 cgtagcggtc aggacgccac catcttcata cgtcgtaacg cgttcccagg taaagccctc
    10081 cgggaagctc tgtttgaaaa aatccgggat accttgagtg tggttgatga aggttttgct
    10141 gccgtacatg aagctggtcg ccaggatgtc gaatgcaaac ggcagcggac cgccttccac
    10201 aactttgata cgcatggttt gggtgccctc atacggtttg ccttcgcctt cgctggtaca
    10261 tttgaagtgg tggttgttta cagtaccttc catgtacagc ttcatgtgca tgttttcttt
    10321 aatcagttct tcacctttgg aaaccatttt tacctcctta ttaattttat gtcgtttaaa
    10381 caaaattatt tgtagaggct gtttcgtcct cacggactca tcagaccgga aagcacatcc
    10441 ggtgacagct gtgcaccccc caactgagag aactcaaagg ttaccccagt tggggcaccg
    10501 agcattgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg
    10561 gacaagaccg gcggtcttaa gtttttt
//
