LOCUS       sequence-178167-        3640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MultiSite Gateway entry clones, first fragment, (attL1, attR5) 
            CCL5/RANTES promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner
            E, Nelson PJ
  TITLE     A vector platform for the rapid and efficient engineering of stable 
            complex transgenes.
  JOURNAL   Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
  PUBMED    27694838
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-3"; volume: "6:34365. doi"; pages: " 
            10.1038/srep34365"
COMMENT     Sequence Label: pENTR221-CCL5.Pro
FEATURES             Location/Qualifiers
     source          1..3640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1703..1826
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1881..1900)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1882..1900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1905..1921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1905..1921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2023..2042)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2034..2843
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2101..2120)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2990..3578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3479..3498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta gctcgagggc tttcatattc tgtaactttt gtgcccaaga aggccttaca
      721 gtgagatggg atcccagtat ttattgagtt tcctcattca taaaatgggg ataataatag
      781 taaatgagtt gacacgcgct aagacagtgg aatagtggct ggcacagata agccctcggt
      841 aaatggtagc caataatgat agagtatgct gtaagatatc tttctctccc tctgcttctc
      901 aacaagtctc taatcaatta ttccacttta taaacaagga aatagaactc aaagacatta
      961 agcacttttc ccaaaggtcg cttagcaagt aaatgggaga gaccctatga ccaggatgaa
     1021 agcaagaaat tcccacaaga ggactcattc caactcatat cttgtgaaaa ggttcccaat
     1081 gcccagctca gatcaactgc ctcaatttac agtgtgagtg tgctcacctc ctttggggac
     1141 tgtatatcca gaggaccctc ctcaataaaa cactttataa ataacatcct tccatggatg
     1201 agggaaagga ggtaagatct gtaatgaata agcaggaact ttgaagactc agtgactcag
     1261 tgagtaataa agactcagtg acttctgatc ctgtcctaac tgccactcct tgttgtcccc
     1321 aagaaagcgg cttcctgctc tctgaggagg accccttccc tggaaggtaa aactaaggat
     1381 gtcagcagag aaatttttcc accattggtg cttggtcaaa gaggaaactg atgagctcac
     1441 tctagatgag agagcagtga gggagagaca gagactcgaa tttccggagg ctatttcagt
     1501 tttcttttcc gttttgtgca atttcactta tgataccggc caatgcttgg ttgctatttt
     1561 ggaaactccc cttaggggat gcccctcaac tggccctata aagggccagc ctgagctgca
     1621 gaggattcct gcagaggatc aagacagcac gtggacctcg cacagcctct cccacaggta
     1681 ctgtacaaag cttatccagc cacaactttg tatacaaaag ttgaacgaga aacgtaaaat
     1741 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     1801 aaaacacaac atatccagtc actatgaatc aactacttag atggtattag tgacctgtac
     1861 tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1921 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     1981 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2041 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     2101 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     2161 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2221 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2281 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     2341 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2401 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2461 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2521 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2581 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2641 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2701 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2761 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2821 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2881 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     2941 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3601 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
