LOCUS       sequence-178176-        4250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human
            IL6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner
            E, Nelson PJ
  TITLE     A vector platform for the rapid and efficient engineering of stable 
            complex transgenes.
  JOURNAL   Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
  PUBMED    27694838
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-3"; volume: "6:34365. doi"; pages: " 
            10.1038/srep34365"
COMMENT     Sequence Label: pENTR221-IL6.Pro
FEATURES             Location/Qualifiers
     source          1..4250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2313..2436
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2491..2510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2492..2510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2515..2531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2515..2531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2633..2652)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2644..3453
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2711..2730)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3600..4188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4089..4108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta cttaagacag gagagcacta aaaatgaaac catccagcca tcctccccca
      721 ttttcatttt cacaccaaag aatcccaccg cggcagagga ccaccgtctc tgtttagaca
      781 atcggtgaag aatggatgac ctcactttcc ccaacaggcg ggtcctgaaa tgttatgcac
      841 gaaacaaaac ttgagtaaat gcccaacaga ggtcactgtt ttatcgatct tgaagagatc
      901 tcttcttagc aaagcaaaga aaccgattgt gaaggtaaca ccatgtttgg taaataagtg
      961 ttttggtgtt gtgcaagggt ctggtttcag cctgaagcca tctcagagct gtctgggtct
     1021 ctggagactg gagggacaac ctagtctaga gcccatttgc atgagaccaa ggatcctcct
     1081 gcaagagaca ccatcctgag ggaagagggc ttctgaacca gcttgaccca ataagaaatt
     1141 cttgggtgcc gacgcggaag cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc
     1201 cttctagctt cttttgattt caaatcaaga cttacaggga gagggagcga taaacacaaa
     1261 ctctgcaaga tgccacaagg tcctcctttg acatccccaa caaagaggtg agtagtattc
     1321 tccccctttc tgccctgaac caagtgggct tcagtaattt cagggctcca ggagacctgg
     1381 ggcccatgca ggtgccccag tgaaacagtg gtgaagagac tcagtggcaa tggggagagc
     1441 actggcagca caaggcaaac ctctggcaca gagagcaaag tcctcactgg gaggattccc
     1501 aaggggtcac ttgggagagg gcagggcagc agccaacctc ctctaagtgg gctgaagcag
     1561 gtgaagaaag tggcagaagc cacgcggtgg caaaaaggag tcacacactc cacctggaga
     1621 cgccttgaag taactgcacg aaatttgagg atggccaggc agttctacaa cagccgctca
     1681 cagggagagc cagaacacag aagaactcag atgactggta gtattacctt cttcataatc
     1741 ccaggcttgg ggggctgcga tggagtcaga ggaaactcag ttcagaacat ctttggtttt
     1801 tacaaataca aattaactgg aacgctaaat tctagcctgt taatctggtc actgaaaaaa
     1861 aatttttttt ttttcaaaaa acatagcttt agcttatttt ttttctcttt gtaaaacttc
     1921 gtgcatgact tcagctttac tctttgtcaa gacatgccaa agtgctgagt cactaataaa
     1981 agaaaaaaag aaagtaaagg aagagtggtt ctgcttctta gcgctagcct caatgacgac
     2041 ctaagctgca cttttccccc tagttgtgtc ttgccatgct aaaggacgtc acattgcaca
     2101 atcttaataa ggtttccaat cagccccacc cgctctggcc ccaccctcac cctccaacaa
     2161 agatttatca aatgtgggat tttcccatga gtctcaatat tagagtctca acccccaata
     2221 aatataggac tggagatgtc tgaggctcat tctgccctcg agcccaccgg gaacgaaaga
     2281 gaagctctat ctcccctcca ggagcccagc tacaactttg tatacaaaag ttgaacgaga
     2341 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     2401 ataatactgt aaaacacaac atatccagtc actatgaatc aactacttag atggtattag
     2461 tgacctgtac tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
     2521 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2581 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2641 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2701 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2761 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2821 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2881 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2941 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3001 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3061 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3121 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3181 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3241 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3301 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3361 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3421 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3481 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3541 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3601 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3661 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3721 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3781 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3841 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3901 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3961 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4021 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4081 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4201 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
