LOCUS       sequence-178177-        3766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MultiSite Gateway entry clones, second fragment (attL5, attL2) Human
            NR1H3 CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner
            E, Nelson PJ
  TITLE     A vector platform for the rapid and efficient engineering of stable 
            complex transgenes.
  JOURNAL   Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
  PUBMED    27694838
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-3"; volume: "6:34365. doi"; pages: " 
            10.1038/srep34365"
COMMENT     Sequence Label: pENTR221-NR1H3
FEATURES             Location/Qualifiers
     source          1..3766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    570..665
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1878..1977)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2007..2026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2008..2026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2031..2047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2031..2047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2149..2168)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2160..2969
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2227..2246)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3116..3704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3605..3624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtaatg ccccactctg ctgggggtac tgcaggggtg gggctggagg ctgcagagcc
      721 cacagccctg ctcaccaggg cagagccccc ttcagaaccc acagagatcc gtccacaaaa
      781 gcggaaaaag gggccagccc ccaaaatgct ggggaacgag ctatgcagcg tgtgtgggga
      841 caaggcctcg ggcttccact acaatgttct gagctgcgag ggctgcaagg gattcttccg
      901 ccgcagcgtc atcaagggag cgcactacat ctgccacagt ggcggccact gccccatgga
      961 cacctacatg cgtcgcaagt gccaggagtg tcggcttcgc aaatgccgtc aggctggcat
     1021 gcgggaggag tgtgtcctgt cagaagaaca gatccgcctg aagaaactga agcggcaaga
     1081 ggaggaacag gctcatgcca catccttgcc ccccagggct tcctcacccc cccaaatcct
     1141 gccccagctc agcccggaac aactgggcat gatcgagaag ctcgtcgctg cccagcaaca
     1201 gtgtaaccgg cgctcctttt ctgaccggct tcgagtcacg ccttggccca tggcaccaga
     1261 tccccatagc cgggaggccc gtcagcagcg ctttgcccac ttcactgagc tggccatcgt
     1321 ctctgtgcag gagatagttg actttgctaa acagctaccc ggcttcctgc agctcagccg
     1381 ggaggaccag attgccctgc tgaagacctc tgcgatcgag gtgatgcttc tggagacatc
     1441 tcggaggtac aaccctggga gtgagagtat caccttcctc aaggatttca gttataaccg
     1501 ggaagacttt gccaaagcag ggctgcaagt ggaattcatc aaccccatct tcgagttctc
     1561 cagggccatg aatgagctgc aactcaatga tgccgagttt gccttgctca ttgctatcag
     1621 catcttctct gcagaccggc ccaacgtgca ggaccagctc caggtagaga ggctgcagca
     1681 cacatatgtg gaagccctgc atgcctacgt ctccatccac catccccatg accgactgat
     1741 gttcccacgg atgctaatga aactggtgag cctccggacc ctgagcagcg tccactcaga
     1801 gcaagtgttt gcactgcgtc tgcaggacaa aaagctccca ccgctgctct ctgagatctg
     1861 ggatgtgcac gaatgaaacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     1921 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     1981 tccagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg
     2041 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2101 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     2161 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     2221 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     2281 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2341 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2401 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2461 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2521 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2581 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2641 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2701 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2761 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2821 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2881 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2941 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     3001 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     3061 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
