LOCUS       sequence-178185-       10901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of CUL4B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, 
            Williams NS, Nijhawan D
  TITLE     Anticancer sulfonamides target splicing by inducing RBM39 
            degradation via recruitment to DCAF15.
  JOURNAL   Science. 2017 Mar 16. pii: eaal3755. doi: 10.1126/science.aal3755.
  PUBMED    28302793
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Mar 16. pii: eaal3755. doi: 10.1126/science.aal3755."
COMMENT     Sequence Label: pLVX CUL4B 3xFlag IRES puro
FEATURES             Location/Qualifiers
     source          1..10901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             376..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5553..5618
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(5650..6224,6225..6227,6228..6236)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5817..5834)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6044..6063
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6256..6855
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6256..6275)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6752..6772
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6869..7457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6922..6942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7340..7351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7664..8297
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8425..8441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8425..8441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8438..8460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8449..8465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8473..8479,8480..8497,8498..8503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8518..8539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8656..8673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8827..9415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8907..8926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9586..10377,10378..10446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10209..10228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10447..10551)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10599..10733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10636..10655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10690..10709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcatg atgtcacagt
     2821 catctggatc aggagatggg aatgatgatg aggctactac ctctaaagac ggtggttttt
     2881 cttcccccag tccctcagct gctgctgctg ctcaggaggt cagatctgcc actgatggta
     2941 ataccagcac cactccgccc acctctgcca agaagagaaa gttaaacagc agcagcagta
     3001 gcagcagtaa cagtagtaac gagagagaag actttgattc cacctcttcc tcctcttcca
     3061 ctcctccttt acaacccagg gattcggcat ccccttcaac ctcgtccttc tgcctggggg
     3121 tttcagtggc tgcttccagc cacgtaccga tacagaagaa gctgcgtttt gaagacaccc
     3181 tggagtttgt agggtttgat gcgaagatgg ctgaggaatc ctcctcctcc tcctcctcat
     3241 cttcaccaac tgctgcaaca tctcagcagc agcaacttaa aaataagagt atattaatct
     3301 cttctgtggc ttcggtgcat catgcaaacg gcctagccaa atcttctacc accgtctcta
     3361 gctttgctaa cagcaaacct ggctctgcta agaagttagt gatcaagaac tttaaagata
     3421 agcctaaatt accagaaaac tacacagatg aaacctggca aaaactgaaa gaagcagtgg
     3481 aagctattca gaatagtact tcaattaagt acaatttaga agaactctac caggctgtag
     3541 aaaatctctg ttcttacaag atttctgcaa acttgtacaa acagctgaga cagatctgcg
     3601 aagatcacat caaagcacag attcatcaat tcagagagga ttcattggat agcgttcttt
     3661 ttttaaagaa gattgataga tgctggcaaa accattgcag acaaatgatc atgatcagga
     3721 gcattttttt gtttctggat agaacttacg ttcttcagaa ttcaatgcta ccctccattt
     3781 gggacatggg actggagtta tttagggctc atattataag tgatcagaaa gtgcagaata
     3841 agacaattga tggcattctt ctcttgattg agagggaaag gaatggtgaa gcaattgata
     3901 gaagtttact tcgaagcctt ttaagcatgc tgtctgattt gcaaatttat caagattctt
     3961 ttgaacaacg atttttggaa gaaactaacc ggctctatgc agctgaaggc caaaaattaa
     4021 tgcaagaaag agaggttcct gaatatctac atcatgttaa caaacgtcta gaagaagaag
     4081 cagacagact tattacttac ttagatcaga ccacccagaa gtcattaatt gctactgtag
     4141 aaaaacaact tctaggtgaa cacttaacag caattcttca gaaaggttta aataacctcc
     4201 ttgatgaaaa ccgaattcaa gatttgtctc ttctgtatca gctcttcagt agagttcgag
     4261 gtggagttca ggttcttttg cagcagtgga tcgaatatat caaggcattt ggcagcacta
     4321 ttgtaattaa tcctgaaaaa gataaaacca tggttcaaga attgctggat tttaaagata
     4381 aggttgacca tataattgat atctgctttc tgaagaatga gaaatttatc aatgccatga
     4441 aagaagcatt tgaaacgttc attaacaaaa gaccaaataa accagctgaa cttatagcta
     4501 agtatgtaga ttcaaaactt cgtgcaggca acaaagaagc tacagatgaa gaacttgaga
     4561 aaatgttgga taaaattatg atcatattta gatttatcta tggcaaggat gtttttgagg
     4621 ccttctataa gaaagattta gccaagcgcc tgttagtcgg aaagagtgca tctgtagatg
     4681 ctgaaaaatc aatgctgtcc aaacttaaac atgaatgcgg agctgctttc accagcaaac
     4741 ttgaaggaat gtttaaagac atggaacttt ctaaagacat catgattcag ttcaaacagt
     4801 atatgcagaa tcagaatgtt ccgggaaata ttgagttaac tgtgaatatc ctgacaatgg
     4861 gctattggcc gacatatgtg cctatggaag ttcatttacc accagagatg gtaaaacttc
     4921 aggagatttt caagacattt tacctaggca aacatagtgg caggaaactt cagtggcagt
     4981 caaccatagg acactgtgtg ttaaaagcag aatttaaaga gggtaaaaag gaactccagg
     5041 tctctctttt tcaaacactg gtgctgctaa tgtttaatga gggagaggag ttcagtttag
     5101 aagagatcaa gcaggcaact ggaatagagg atggagagtt aaggagaaca ctgcagtcat
     5161 tagcctgtgg caaagctaga gttctggcga aaaatccaaa gggcaaagac attgaagatg
     5221 gtgacaagtt catttgtaat gatgatttca aacataaact tttcaggata aagatcaatc
     5281 aaatccagat gaaagaaacg gttgaagaac aagcaagcac tacagaaaga gtatttcaag
     5341 acagacagta tcaaattgat gctgcaattg ttcgaattat gaagatgaga aagacactta
     5401 gccacaatct ccttgtttca gaagtgtaca accagttgaa atttccagta aagcctgctg
     5461 atcttaagaa gagaatagaa tctttaattg accgggacta catggaaaga gataaagaaa
     5521 atccaaacca gtacaactat attgcaggta gcgactataa agaccacgac ggggattaca
     5581 aggaccatga cattgattac aaggacgatg atgataaata gactagttct agagcggccg
     5641 cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     5701 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5761 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5821 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5881 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5941 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6001 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6061 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6121 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6181 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca
     6241 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6301 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6361 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6841 aagcccggtg cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     6901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7261 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7441 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     7501 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7561 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     7681 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7741 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     7801 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     7861 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     7921 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     7981 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8041 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8101 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8161 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8221 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8281 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8341 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc
     8401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10561 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    10621 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10681 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10741 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10801 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10861 gaaattgtat ttgttaaata tgtactacaa acttagtagt t
//
