LOCUS       sequence-178186-       12051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Turning off/down miR394 in Soybean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 12051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: TF101-STTM-miR394
FEATURES             Location/Qualifiers
     source          1..12051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..411
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    569..745
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1307..1325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1308..1325)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(2086..2091,2092..2108,2109..2114))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2384..2400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2384..2400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2397..2419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2408..2424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2432..2438,2439..2456,2457..2462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2477..2498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2656..2680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4011..4640
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5069..6142
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6208..6402
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6638..6660)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6746..6886
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6801..6820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6901..6918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7072..7660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7906..8697)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9218..9242
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     9442..9464
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9456..9473
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9457..9473
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(10043..10591)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10743..11087)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11691..11710
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11691..11709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacgcacaat cccaccccta ctccaaaaat gtcaaagata ctgtctcaga agaccaaagg
       61 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca
      121 gctatctgtc acctcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      181 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
      241 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      301 caagtggatt gatgtgacat ctccactgac ataagggatg acgcacaacc ccactatcct
      361 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct
      421 tggaggtgac ctaagaatgc caagttgttg ttgttatggt ctaatttaaa tatggtctaa
      481 agaagaagaa tggaggtgga cctaagaatg ccaagaattc ggtacgctga aatcaccagt
      541 ctctctctac aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc
      601 gataagggaa attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
      661 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
      721 aaaatccagt actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata
      781 aaccagacac gagacgacta gacctggagc ccagacgccg ttcgaagcta gaagtaccgc
      841 ttaggcagga ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc
      901 gtaggtttgg tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata
      961 aggttgcagg ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac
     1021 ttatactata cgctaaggga atgattgtat ttatacccta taccccctaa taacccctta
     1081 tcaatttaag aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat
     1141 aggtctatga ccaaaactca agaggataaa acctcaccaa aatacgaaag agtccttaac
     1201 tctaaagata aaagatcttt caagatcaaa actagttccc tcacaccggt gacggggatc
     1261 gcatgcgacc tctagaccat cacattggag cttcagctgc tcgagttcta tagtgtcacc
     1321 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tcttacgtcg
     1381 acgtaccaat tacgccccgc cctgtcactc atcgcagtac tgttgtaatt cattaagcat
     1441 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1501 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1561 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     1621 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1681 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1741 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1801 taccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca tcaggcgggc
     1861 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1921 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg accgaaatgc
     1981 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttc
     2041 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcattaa agcttatcga
     2101 tgataagctg tcaaacatga gaatttagcc cgcctaatga gcgggctttt tgttgtgatc
     2161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttcccccctg
     2281 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2341 ttcttaatta agacccgggt accgagctcg aattcgtaat catgtcatag ctgtttcctg
     2401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2581 gaggcggttt gcgtattgga gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
     2641 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     2701 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2761 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttaaagtact ttaaagtact
     2821 ttaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     2881 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     2941 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     3001 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     3061 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     3121 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     3181 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     3241 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     3301 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     3361 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     3421 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     3481 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     3541 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     3601 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     3661 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     3721 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     3781 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     3841 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     3901 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     3961 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     4021 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     4081 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     4141 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     4201 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     4261 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     4321 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     4381 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     4441 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     4501 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     4561 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     4621 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     4681 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     4741 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     4801 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     4861 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     4921 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     4981 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     5041 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     5101 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     5161 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     5221 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     5281 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     5341 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     5401 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     5461 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     5521 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     5581 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     5641 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     5701 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     5761 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     5821 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     5881 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     5941 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     6001 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     6061 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     6121 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     6181 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     6241 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     6301 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     6361 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     6421 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     6481 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     6541 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     6601 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6661 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6721 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6781 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6841 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7501 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga
     7741 tatatctccc aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt
     7801 gacctgatag tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc
     7861 cgcgccgcga agcggcgtcg gcttgaacga atttctagct agacattatt tgccgactac
     7921 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     7981 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     8041 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     8101 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     8161 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     8221 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     8281 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     8341 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     8401 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     8461 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     8521 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     8581 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     8641 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt cccccatgat
     8701 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     8761 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     8821 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
     8881 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg
     8941 aaccgaacag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     9001 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     9061 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     9121 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     9181 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     9241 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     9301 acgccgaatt gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9361 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     9421 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     9481 taattcgctt caagacgtgc tcaaatcact atttccacac ccctatattt ctattgcact
     9541 cccttttaac tgttttttat tacaaaaatg ccctggaaaa tgcactccct ttttgtgttt
     9601 gtttttttgt gaaacgatgt tgtcaggtaa tttatttgtc agtctactat ggtggcccat
     9661 tatattaata gcaactgtcg gtccaataga cgacgtcgat tttctgcatt tgtttaacca
     9721 cgtggatttt atgacatttt atattagtta atttgtaaaa cctacccaat taaagacctc
     9781 atatgttcta aagactaata cttaatgata acaattttct tttagtgaag aaagggataa
     9841 ttagtaaata tggaacaagg gcagaagatt tattaaagcc gcgtaagaga caacaagtag
     9901 gtacgtggag tgtcttaggt gacttaccca cataacataa agtgacatta acaaacatag
     9961 ctaatgctcc tatttgaata gtgcatatca gcatacctta ttacatatag ataggagcaa
    10021 actctagcta gattgttgag cagatctcgg tgacgggcag gaccggacgg ggcggtaccg
    10081 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
    10141 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
    10201 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
    10261 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
    10321 cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga
    10381 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
    10441 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
    10501 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
    10561 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cccgttcgta aatggtgaaa
    10621 attttcagaa aattgctttt gctttaaaag aaatgattta aattgctgca atagaagtag
    10681 aatgcttgat tgcttgagat tcgtttgttt tgtatatgtt gtgttgagaa ttaattctcg
    10741 aggtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
    10801 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
    10861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    10921 gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt
    10981 tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat
    11041 ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt
    11101 cttctgagac tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt
    11161 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    11221 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
    11281 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
    11341 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
    11401 gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt
    11461 agacaagtgt gtcgtgctcc accatgttga cctgcaggca tgcaagcttg catgcctgca
    11521 ggtcgactct agagagttaa ttaagtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11581 tctcaaggag atcctttgga tcacaaaaaa aaagcccgct cattaggcgg gttaaattcc
    11641 cgggtactgc agccgattca ttaatgcagg ttaacctggc ttatcgaaat taatacgact
    11701 cactataggg agaccggcag atctgatccc tactccaaaa atgtcaaaga tacggtctca
    11761 gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
    11821 ttccattgcc cagctatctg tcacttcatc gaaaggacag tggaaaagga aggtggctcc
    11881 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
    11941 ggtcccaaag atggaccccc acccacgagg agcatcgtgg gaaaagaaga cgttccaacc
    12001 acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga t
//
