LOCUS       sequence-178188-       12002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Turning off/down miR156/157 in Soybean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 12002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: TF101-STTM-miR156/157
FEATURES             Location/Qualifiers
     source          1..12002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..411
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    572..748
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1310..1328)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1311..1328)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1399..2058)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1973..1992
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2095..2100,2101..2117,2118..2123))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2404..2420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2404..2420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2417..2439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2428..2444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2452..2458,2459..2476,2477..2482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2497..2518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2676..2700
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4031..4660
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5089..6162
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6228..6422
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6658..6680)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6766..6906
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6821..6840
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6921..6938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7092..7680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7926..8717)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9238..9262
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     9462..9484
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9476..9493
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9477..9493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(10063..10611)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10763..11107)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11642..11661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11642..11660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacgcacaat cccaccccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg
       61 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggctt ccattgccca
      121 gctgtctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      181 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
      241 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      301 caagtggatt gatgtgacgt ctccactgac gtaagggatg acgcacaatc ccactatcct
      361 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct
      421 tagtgctcac tcctatcttc tgtcagttgt tgttgttatg gtctaattta aatatggtct
      481 aaagaagaag aatgtgctct ctatctactt ctgtcaagaa ttcggtacgc tgaaatcacc
      541 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
      601 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
      661 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
      721 accaaaatcc agtactaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat
      781 ataaaccaga cacgagacga ctaaacctgg agcccagacg ccgttcgaag ctagaagtac
      841 cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta cgttgactcc
      901 cccgtaggtt tggtttaaat atgatgaagt ggacggatgg aaggaggaag acaaggaagg
      961 ataaggttgc aggccctgcg caaggtaaga agatggaaat ttgatagagg tacgctacta
     1021 tacttatact atacgctaag ggaatgcttg tatttatacc ctataccccc taataacccc
     1081 ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccatg
     1141 aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagttctt
     1201 aactctaaag ataaaagatc tttcaagatc aaaactagtt ccctcacacc ggtgacgggg
     1261 atcgcatgcg atctctagac catcacattg gagcttcagc tactcgagtt ctatagtgtc
     1321 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     1381 tcgacaggcc tgtaccaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1441 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     1501 cggcatcagc accttgtcgc cttgcgtata gtatttgccc atggtgaaaa cgggggcgaa
     1561 gaagttgtcc atattggcta cgtttaaatc aaaactggtg aaactcaccc agggattggc
     1621 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1681 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1741 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1801 atcccatatc accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat
     1861 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1921 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1981 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2041 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcattaaa
     2101 acttatcgat gataagctgt caaacatgag aatttagccc gcctaatgag cgggcttttt
     2161 gttgtgatcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2281 acgtagcgat cgcgatcgct acgtaacgcc tggtatcttt atagtcctgt cgcccgggaa
     2341 tttaacccgc ctaatgagcg ggcttaatta agacccgggt accgagctcg aattcgtaat
     2401 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2461 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2521 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2581 aatcggccaa cgcgcgggga gaggcggttt gcgtattgga gcttgagctt ggatcagatt
     2641 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     2701 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     2761 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     2821 ttaaagtact ttaaagtact ttaaagtact ttgatccaac ccctccgctg ctatagtgca
     2881 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     2941 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     3001 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     3061 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3121 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3181 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3241 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3301 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3361 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3421 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3481 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3541 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3601 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     3661 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     3721 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     3781 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     3841 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     3901 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     3961 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     4021 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     4081 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4141 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4201 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4261 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4321 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4381 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4441 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4501 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4561 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4621 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     4681 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     4741 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     4801 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     4861 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     4921 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     4981 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     5041 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     5101 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     5161 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     5221 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     5281 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     5341 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     5401 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     5461 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     5521 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     5581 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     5641 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     5701 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     5761 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     5821 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     5881 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     5941 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     6001 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     6061 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     6121 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     6181 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     6241 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     6301 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     6361 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     6421 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     6481 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     6541 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     6601 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6661 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6721 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     6781 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     6841 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6901 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7501 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7741 gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta tgcacgctta
     7801 aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg tgcttagtgc
     7861 atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga atttctagct
     7921 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     7981 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     8041 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     8101 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     8161 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     8221 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     8281 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     8341 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     8401 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     8461 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     8521 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     8581 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     8641 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     8701 atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
     8761 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     8821 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
     8881 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     8941 tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg tgcccgaatt
     9001 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     9061 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     9121 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     9181 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     9241 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     9301 gtttttaatg tactgaatta acgccgaatt gctctagcat tcgccattca ggctgcgcaa
     9361 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9421 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     9481 aacgacggcc agtgccaagc taattcgctt caagacgtgc tcaaatcact atttccacac
     9541 ccctatattt ctattgcact cccttttaac tgttttttat tacaaaaatg ccctggaaaa
     9601 tgcactccct ttttgtgttt gtttttttgt gaaacgatgt tgtcaggtaa tttatttgtc
     9661 agtctactat ggtggcccat tatattaata gcaactgtcg gtccaataga cgacgtcgat
     9721 tttctgcatt tgtttaacca cgtggatttt atgacatttt atattagtta atttgtaaaa
     9781 cctacccaat taaagacctc atatgttcta aagactaata cttaatgata acaattttct
     9841 tttagtgaag aaagggataa ttagtaaata tggaacaagg gcagaagatt tattaaagcc
     9901 gcgtaagaga caacaagtag gtacgtggag tgtcttaggt gacttaccca cataacataa
     9961 agtgacatta acaaacatag ctaatgctcc tatttgaata gtgcatatca gcatacctta
    10021 ttacatatag ataggagcaa actctagcta gattgttgag cagatctcgg tgacgggcag
    10081 gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca
    10141 gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc
    10201 gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc
    10261 ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg
    10321 gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt
    10381 ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg
    10441 atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt
    10501 acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat
    10561 gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc
    10621 cccgttcgta aatggtgaaa attttcagaa aattgctttt gctttaaaag aaatgattta
    10681 aattgctgca atagaagtag aatgcttgat tgcttgagat tcgtttgttt tgtatatgtt
    10741 gtgttgagaa ttaattctcg aggtcctctc caaatgaaat gaacttcctt atatagagga
    10801 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    10861 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    10921 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga tggcctttcc
    10981 tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct tcacaataaa
    11041 gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc tttgttgaaa
    11101 agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg agtagacaag
    11161 tgtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac
    11221 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    11281 agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt aggagccacc
    11341 ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga atccgaggag
    11401 gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt
    11461 atctttgata tttttggagt agacaagtgt gtcgtgctcc accatgttga cctgcaggca
    11521 tgcaagcttg catgcctgca ggtcgactct agagagttaa ttaagcccgc tcattaggcg
    11581 ggttaaattc ccgggtactg cagccgattc attaatgcag gttaacctgg cttatcgaaa
    11641 ttaatacgac tcactatagg gagaccggca gatctgatcc ctactccaaa aatgtcaaag
    11701 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atttcgggaa
    11761 acctcctcgg atcccgttgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
    11821 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt caagatgcct
    11881 ctgccgacgg tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
    11941 acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact gacgtaaggg
    12001 at
//
