LOCUS       sequence-178198-        8310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CjCas9 with His tag in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung 
            MH, Kim S, Kim JH, Kim JH, Kim JS
  TITLE     In vivo genome editing with a small Cas9 orthologue derived from 
            Campylobacter jejuni.
  JOURNAL   Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500.
  PUBMED    28220790
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-21"; volume: "8:14500. doi"; pages: " 
            10.1038/ncomms14500"
COMMENT     Sequence Label: pET-CjCas9
FEATURES             Location/Qualifiers
     source          1..8310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    138..277
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     193..212
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(292..309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(463..1051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(543..562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1162..1181)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1173..1988
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1173..1988
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1246..1265)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(2080..2535)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2217..2238)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2429..2448
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(2572..2619)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2615..2633
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(2686..2703)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2725..5673)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Campylobacter jejuni 
                     Type II CRISPR/Cas system (Kim et al., 2016)"
                     /label=CjCas9
                     /translation="ARILAFDIGISSIGWAFSENDELKDCGVRIFTKVENPKTGESLAL
                     PRRLARSARKRLARRKARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYELR
                     FRALNELLSKQDFARVILHIAKRRGYDDIKNSDDKEKGAILKAIKQNEEKLANYQSVGE
                     YLYKEYFQKFKENSKEFTNVRNKKESYERCIAQSFLKDELKLIFKKQREFGFSFSKKFE
                     EEVLSVAFYKRALKDFSHLVGNCSFFTDEKRAPKNSPLAFMFVALTRIINLLNNLKNTE
                     GILYTKDDLNALLNEVLKNGTLTYKQTKKLLGLSDDYEFKGEKGTYFIEFKKYKEFIKA
                     LGEHNLSQDDLNEIAKDITLIKDEIKLKKALAKYDLNQNQIDSLSKLEFKDHLNISFKA
                     LKLVTPLMLEGKKYDEACNELNLKVAINEDKKDFLPAFNETYYKDEVTNPVVLRAIKEY
                     RKVLNALLKKYGKVHKINIELAREVGKNHSQRAKIEKEQNENYKAKKDAELECEKLGLK
                     INSKNILKLRLFKEQKEFCAYSGEKIKISDLQDEKMLEIDHIYPYSRSFDDSYMNKVLV
                     FTKQNQEKLNQTPFEAFGNDSAKWQKIEVLAKNLPTKKQKRILDKNYKDKEQKNFKDRN
                     LNDTRYIARLVLNYTKDYLDFLPLSDDENTKLNDTQKGSKVHVEAKSGMLTSALRHTWG
                     FSAKDRNNHLHHAIDAVIIAYANNSIVKAFSDFKKEQESNSAELYAKKISELDYKNKRK
                     FFEPFSGFRQKVLDKIDEIFVSKPERKKPSGALHEETFRKEEEFYQSYGGKEGVLKALE
                     LGKIRKVNGKIVKNGDMFRVDIFKHKKTNKFYAVPIYTMDFALKVLPNKAVARSKKGEI
                     KDWILMDENYEFCFSLYKDSLILIQTKDMQEPEFVYYNAFTSSTVSLIVSKHDNKFETL
                     SKNQKILFKNANEKEVIAKSIGIQNLKVFEKYIVSALGEVTKAEFRQREDFKK"
     CDS             complement(5701..5721)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5731..5757)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(5731..5757)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5761..5778)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             5797..5819
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    5834..5858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5858..5877)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5859..5877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5961..5980)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6186..6263
                     /gene="lacI"
                     /label=lacI promoter
     CDS             6264..7346
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6283..6302)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(8155..8310,1..36)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
      181 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattgc ggtgtgaaat
      241 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
      301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      901 aagracagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt
     1141 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
     1201 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     1261 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     1321 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1381 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1441 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
     1501 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1561 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1621 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1681 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1741 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1801 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1861 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1921 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1981 ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2041 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     2101 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     2161 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     2221 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2281 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     2341 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2401 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     2461 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     2521 cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca gcaaaaaacc
     2581 cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc
     2641 agccaactca gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg
     2701 gtgctcgagt tattaaggtc cggacttttt gaagtcctct ctctgccgga actcggcctt
     2761 tgtcacttct cccagggcgg acacgatgta cttctcgaac accttcagat tctggatgcc
     2821 gatgctcttg gcgatcactt ctttctcgtt ggcgttcttg aacaggatct tctggttctt
     2881 gctcagtgtc tcgaacttgt tgtcgtgctt agacacgatc agggacacgg tgctgctggt
     2941 gaaggcgttg tagtagacga actcgggttc ctgcatgtcc ttggtctgga tcaggatcag
     3001 ggagtccttg tacaggctaa agcagaactc gtagttctcg tccatcagaa tccagtcctt
     3061 gatctcgccc ttcttggacc gggccacggc cttgttgggc agcaccttca gggcgaagtc
     3121 catggtgtag atgggcacgg cgtagaactt gttggtcttt ttgtgcttga agatgtccac
     3181 ccggaacatg tccccgttct tcacgatctt gccgttcact tttctgatct tgcccagctc
     3241 gagggccttc agcacgcctt ctttgccgcc gtaggactgg tagaactctt cctcttttct
     3301 gaaggtttcc tcgtgcaggg cgccagaggg cttcttccgc tcgggcttgg acacgaagat
     3361 ctcgtcgatc ttatccagca ccttctgccg gaagccgctg aagggctcga agaacttccg
     3421 cttgttcttg tagtccagct cagagatctt cttggcgtac agctcggcgc tgttgctttc
     3481 ctgttctttc ttgaagtcgg agaaggcctt cacgatgctg ttgttggcgt aggcaatgat
     3541 cacggcgtcg atggcgtggt gcagatggtt gttccgatcc ttggcgctga agccccaggt
     3601 gtgtctcagg gcgctggtca gcatgccgct cttggcttcc acgtgcacct tggagccttt
     3661 ctgggtatcg ttcagcttgg tgttctcgtc gtcggacagg ggcaggaaat ccaggtagtc
     3721 ctttgtgtag ttcagcacca gccgggcgat ataccgggtg tcgttcaggt tccggtcctt
     3781 gaagttcttc tgctctttgt ccttgtaatt cttgtccagg attctcttct gtttcttggt
     3841 gggcaggttc ttggccagca cctcgatttt ctgccacttg gcgctgtcgt tgccgaaggc
     3901 ctcgaagggg gtctggttca gtttttcctg gttctgtttg gtgaacacca gcaccttgtt
     3961 catgtagctg tcgtcgaagg accggctgta ggggtagatg tggtcgattt ccagcatctt
     4021 ctcgtcctgc agatcgctga tcttgatctt ctcgccgctg taggcgcaga actctttctg
     4081 ttctttgaac agccgcagct tcaggatgtt cttgctgttg atcttcagtc ccagcttctc
     4141 gcattccagc tcagcgtcct tcttggcctt gtagttttcg ttctgctctt tctcgatctt
     4201 ggcccgctgg ctgtggttct tgcccacttc ccgggccagc tcgatgttga tcttgtgcac
     4261 cttgccgtat ttcttgagca gggcattcag caccttccgg tattctttga tggcccgcag
     4321 caccacgggg ttggtcactt cgtccttgta gtaggtttcg ttgaaggcgg gcaggaagtc
     4381 cttcttgtcc tcgttgatgg ccaccttcag gttcagctcg ttgcaggcct cgtcgtactt
     4441 cttgccttcc agcatcaggg gggtgaccag cttcagagcc ttgaagctga tgttcaggtg
     4501 atccttgaat tccagcttgc tcaggctgtc gatctggttc tgattcaggt cgtatttggc
     4561 cagggccttc ttcagcttaa tctcgtcctt gatcagggtg atgtccttgg cgatctcatt
     4621 cagatcgtcc tggctcaggt tgtgctcgcc cagggccttg atgaactctt tgtacttctt
     4681 gaactcgatg aagtaggtgc ccttctcgcc cttaaactcg tagtcgtcgc tcaggcccag
     4741 cagcttcttg gtctgcttgt aggtcagggt gccgttcttc agcacctcat tcagcagggc
     4801 gttcaggtcg tccttggtgt acaggatgcc ctcggtgttc ttcagattgt tcagcaggtt
     4861 gatgatccgg gtcagggcca cgaacatgaa ggccaggggg ctgttcttgg gggctctctt
     4921 ctcgtcggtg aagaagctgc agttgcccac gaggtggctg aagtccttca gggctctctt
     4981 gtagaaggcg acgctcagca cctcttcctc gaatttcttg ctgaagctga agccgaactc
     5041 tctctgcttc ttgaagatca gcttcagctc atccttcagg aagctctggg cgatacaccg
     5101 ctcgtagctt tctttcttgt tccgcacgtt ggtgaattct ttgctgttct ctttgaactt
     5161 ctggaagtac tctttgtaca ggtactcgcc cacggactgg tagttggcca gcttttcctc
     5221 gttctgcttg atggccttca ggatggcgcc cttctctttg tcgtcgctgt tcttgatgtc
     5281 gtcgtagcct ctccgcttgg caatgtgcag gatcactctg gcgaagtcct gcttgctcag
     5341 cagctcgttc agggcccgga accgcagctc gtaggggctg atcaggctgc ccttgtaggc
     5401 cttggccagg gactcgtcga agctctggta gtcctcgtag ttcagcttga actcgttggc
     5461 gatcaggtgc ttcaggtggt tcagccgggc cttccgtctg gccagtctct tccgggcgct
     5521 tctggccagc cttctgggca gggccaggct ctcgccggtc ttggggtttt ccaccttggt
     5581 gaagatccgc acgccgcagt ccttcagctc gtcgttctcg ctgaaggccc agccgatgct
     5641 gctgatgccg atgtcgaagg ccaggattct ggccggtttt tccccgggaa ttcggatccc
     5701 tacctttctc ttcttttttg gaggcaattc ggcgtagtcg ggcacgtcgt aggggtacac
     5761 atgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa
     5821 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
     5881 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     5941 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     6001 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     6061 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     6121 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga
     6181 tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
     6241 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     6301 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     6361 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     6421 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     6481 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     6541 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     6601 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     6661 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     6721 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
     6781 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     6841 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     6901 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     6961 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     7021 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     7081 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     7141 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     7201 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     7261 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7321 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat
     7381 taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     7441 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     7501 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     7561 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     7621 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     7681 gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     7741 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     7801 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     7861 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     7921 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     7981 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     8041 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     8101 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     8161 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     8221 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     8281 gaccacgctg atgagcttta ccgcagctgc
//
