LOCUS       sequence-178202-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express shRNA against human Nup133.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Tie HC, Madugula V, Lu L
  TITLE     The development of a single molecule fluorescence standard and its 
            application in estimating the stoichiometry of the nuclear pore 
            complex.
  JOURNAL   Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 
            10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6.
  PUBMED    27613095
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2016.09.005"; journalName: "Biochem Biophys Res 
            Commun"; date: "2016-09-30- 30"; volume: "478"; issue: "4"; pages: 
            "1694-9"
COMMENT     Sequence Label: pSUPER-retro-puro-shNup133-1
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        26..240
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     161..180
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(323..739)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(339..355)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(371..393)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gcgcctcccc tacccggtag aattcgaacg ctgacgtcat caacccgctc caaggaatcg
       61 cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc
      121 tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa
      181 tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac
      241 agatcccctg ttcagggaca aatataattc aagagattat atttgtccct gaacattttt
      301 ctcgagagat cgatctaatt ccggcgccta gagaaggagt gagggctgga taaagggagg
      361 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
      421 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
      481 cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga
      541 ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga
      601 cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta
      661 ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg
      721 gagtggtaac agtctggccc taattttcag acaaatacag a
//
