LOCUS       sequence-178215-        6681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of heterologous genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Pertel T, Hausmann S, Morger D, Zuger S, Guerra J, Lascano J, 
            Reinhard C, Santoni FA, Uchil PD, Chatel L, Bisiaux A, Albert ML, 
            Strambio-De-Castillia C, Mothes W, Pizzato M, Grutter MG, Luban J
  TITLE     TRIM5 is an innate immune sensor for the retrovirus capsid lattice.
  JOURNAL   Nature. 2011 Apr 21;472(7343):361-5. doi: 10.1038/nature09976.
  PUBMED    21512573
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-04-21- 21"; volume: "472"; issue: "7343"; 
            pages: "361-5"
COMMENT     Sequence Label: pALPS
FEATURES             Location/Qualifiers
     source          1..6681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..87)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        72..401
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      252..387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     314..333
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        413..792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        794..992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     947..967
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1010..1190
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1237..1362
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1859..2092
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2277..2321
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2619..2736
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2873..3280
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3399..3987
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3452..3472)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3870..3881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4505..4685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4756..5344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4836..4855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5515..6306,6307..6375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6138..6157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6376..6480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6555..6574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
       61 cgctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      121 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      181 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      241 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      301 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
      361 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgcg ttgacattga
      421 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      661 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      721 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      781 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      841 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      901 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      961 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     1021 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1081 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1141 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1201 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1261 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1321 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1381 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1441 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1501 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1561 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1621 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1681 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     1741 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1801 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1861 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1921 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1981 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2041 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2101 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     2161 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2221 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2281 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2341 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2401 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2461 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2521 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2581 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2641 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2701 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2761 gatccagttt ggttaattaa ctgcagcccc gataaaataa aagattttat ttagtctcca
     2821 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc
     2881 attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg
     2941 tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc
     3001 ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca
     3061 gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca
     3121 ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct
     3181 tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc
     3241 actcggcgcg ccagtcctcc gacagactga gtcgcccggg ggtctagaag cgctggatcc
     3301 gtttaaacgc ggccgcccag cacagtggct cgagccgcgg gttaactggc cagaattcac
     3361 gcgtatcgat accggtggcc cctggggccg cgatcgctaa tcaacctctg gattacaaaa
     3421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3481 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3541 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3601 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3661 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3721 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3781 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3841 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3901 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3961 ggatctccct ttgggccgcc tccccgctta atcgcgtcga gacctagaaa aacatggagc
     4021 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     4081 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     4141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4201 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     4261 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     4321 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     4381 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     4441 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     4501 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4561 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4621 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4681 tagcagggcc cgtttcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5161 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5821 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5881 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5941 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6001 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6061 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6121 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6181 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6241 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6301 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6361 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6421 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6481 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     6541 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     6601 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     6661 ggcttgaccg acaattgcat g
//
