LOCUS       sequence-178228-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized AsCpf1 variant that recognizes TATV PAMs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, 
            Nishimasu H, Nureki O, Crosetto N, Zhang F
  TITLE     Engineered Cpf1 variants with altered PAM specificities.
  JOURNAL   Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
  PUBMED    28581492
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900."
COMMENT     Sequence Label: pcDNA3.1-hAsCpf1(TATV) (pY220)
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     214..234
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(243..671)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(353..374)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     565..584
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    717..941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     930..947
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1021..1101)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(1108..1155)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(1156..5073)
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNVEKNRGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      1427..1436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5098..5118)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5121..5130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5165..5184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5166..5184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5229..5432)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5258..5278)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5433..5812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5984..6003
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6078..6182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6183..6251,6252..7043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6401..6420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7214..7802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7703..7722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7956..7973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8090..8111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8126..8131,8132..8149,8150..8156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8164..8180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8169..8191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8188..8204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8188..8204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8253..8374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8264..8283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8318..8337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8548..9342)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8659..8678)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9269..9288
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9409..9509,1..229))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9423..9509,1..49)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9477..9496)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
       61 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
      121 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
      181 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
      241 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
      301 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
      361 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
      421 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
      481 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
      541 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
      601 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
      661 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
      721 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
      781 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
      841 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
      901 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
      961 ttaaacgggc cctctagact cgagcggccg ccactgtgct ggatatctgc agaattctta
     1021 ggcatagtcg gggacatcat atgggtatgc ataatcaggc acgtcgtagg gataagcgta
     1081 atctggaaca tcgtatgggt aggatccctt tttctttttt gcctggccgg cctttttcgt
     1141 ggccgccggc cttttgttgc gcagctcctg gatgtaggcc agccagtcct gattggagat
     1201 gccgttctgc agcttcagat ccttgctctc cttcaggtga ttcagcagca gctggccctt
     1261 cagggcgatg tggtaggcgc cattggcatc ggcgtccatg ggccactctg ggttctgaaa
     1321 ccgggagtcg aagcacacgc cattcagatc gcgcacgggg ctgttgatat agtcctcgcc
     1381 tgtggcggca ttggagttcc gcatctgcag cacgctgcgg atcagggcca ccatggtgtc
     1441 gatggcgtga gaatcgtcat tctccagcag ctttggcagg atgttggagc catccctgaa
     1501 cacgatgccc ttctcctcca gcagggcgat cagctcgttg gcaggataca ggtcccggta
     1561 tctgccggtg aatctgtgat tctcgatcac tggcacgatt ctcttgccgg cgatgaaagg
     1621 ggtgcccttg gcgtcaaact gtgtctcgtt cttctcgaac acgatatccc atgcaggcat
     1681 aaagccgggc aggcccctct ggaaggacag atttctgttc atcttaaagt gcaggatgaa
     1741 gtcgccggtt ttcacgtcgt agtgcagaaa gtcgaagccc tccaggaagt gcttgcggct
     1801 ctcgtgattc ttgatggttt tccacacgaa ggggtccacg aagccggtca ggggatcgat
     1861 cttagatgta tatggggcag gcacgtaaaa caggaagcca gactgggtgc ccatcttggc
     1921 aaaggaggtg aactggtctg tcagctggta tgggttcagc acgcctccca ctttctctgc
     1981 tggatagtcc ttcagcacca ggcaattcag cttatcgatc agcatcttct cgaactgctg
     2041 gtacacggcc ttctcggcga tgccggtcct cttgctctta aagccgaaat tcaggttctc
     2101 cagcaccacc acggcctggt agtggatcat caggtccacg atctcgtgga tgacctggct
     2161 cagatagccc tgcttcagat ccttgattgt gcccaccaca gaccaggcct gccttgctgc
     2221 caccctctcc ttctccctgt tgtccagctt cttctggtaa tcaaactgct ggatggtgtt
     2281 caggctccgc tgctccagga tcttgccggt ggagtcgatc actgtgatat agatcaggtt
     2341 tctctcgccc cgatcgatgc cgatgatagg tgtctcgggg tgctccttca ggtaggcatt
     2401 caccctctgg ttgaacttag atggggaatt ggcggcctga tagttcagtg tgataggcac
     2461 gtggaaaaag aacttgtcgc tggtaaagcg cctatccttg atgatctcgt gagacacctc
     2521 cttggtgatc acgttgggca gcagggccct ggcctcatca gacaggtcgt gggacagtct
     2581 gtgattcaca tagtcgtaca gctcctggta cagggtgtcg gggattgggg ttttctgatc
     2641 cttcagcttc ttgttcagca tcttctctcc cagccggtgt gccatcctct tcatcctgga
     2701 cttagggcgg tagaacagct cggcctggcc attcagcttg atgcttgtct tggccaggtt
     2761 ctctggagaa aacagaccgg tccaatacag tgtgtgcaga ttaggcttgc cgtggtggcc
     2821 cttggcaaag tccttgttat agatctggaa caggtacagc ttgcctgtct ccacggcatc
     2881 catgatctcc ttctcggcga ttctctggaa gctgatgtgg tacagcaggg gattcagctc
     2941 ggcatagtac tcgcccaggt ccttatactg agaggatggc cgcaggctag acagatcgat
     3001 agaggttgtc ttggtatact tggacagaaa atcccttgtg aagtcgatcc acttgcacag
     3061 ggcctctctg tagcccttct ggtcgccggt tttcttggcg tacgctgtct gaaacttctt
     3121 tggctccttc tcaggattgt tcaggtcgta gatctccttt gtgatctcca gaggctcgat
     3181 gaaattgttg gacagcagga tgggggttgt gtgggtctga aagtgggcgg tcaccgcctt
     3241 cagctgggtg ctgcactttg ggatcatctt ggcggcatcc ggaaagtagt catagtacat
     3301 cttatcaaag ccctcgctgg ttttctctgt gggctcgaag ctgagggcct tatacctgcc
     3361 cttctgcttt ggcatgatgc ccagatagta caggccgttc ttcacaaaca ggatggcgcc
     3421 ccggttcttc tccacattca cgtcccagcc ccgggccagt gtaggcatct gaaagttcag
     3481 cttgaacttc tccacggagt agggcttctt ggtggcataa tttctggcct tgttgtagaa
     3541 gctcagagaa ggctccatct ccagcttgat gccggtcagc cgggcagaga actcggggtc
     3601 cacctcgttg gactcatcca cggcaaacca gtccagcagg tggtacaggc ccagcaggct
     3661 gtccagctga gacttcagga tctccttctc ctcctgcttc ttcagggttg taggcagtgg
     3721 ctgatccagg gcggcgtgtg cgtgggacag gatctcgctg gttttctgct tgaaggcctc
     3781 gctcagctcc ttgcctgcgg cagagatgat ctcctgcagg ttgatatcct cgtgcttcag
     3841 gctgcgctgc accttctcct tggcagactt ggtgatcttg cctgtcagct cggagattct
     3901 ccgctcatac agggcattcc tcagtgtatc ccagtggtcg cacagggcgc tgctgattgt
     3961 ctccagcttc ttgtggctga tgaagatgtg tgtcaggtcg atgctgttca gctcgttaaa
     4021 cagggcctcg gctgtctcca gcacgttctc gtttctcagc agtgtcttgt acttgcagaa
     4081 ggactggatc acttcctcgt cgctcttaaa ctcctccagg atgaaagaca gggtgttcct
     4141 atcggacagg atctgcttaa acagggggat gaatctgtgt ggcagggagg cgatgatgtg
     4201 ggctgtctca tcattcttct ggatggccag attcagcacc tcgttcaggc ccttgatctt
     4261 ctcggtgcct gcctcccgag agattcctcc cagcagctgg ttatacaggt cgatctgggt
     4321 ctgtgtcagc agctggttat aaaaagggaa ggaaaacacc tcctcgatgg aggtgctcac
     4381 gaagatgccg atggccttct tcacgttctc aaagtgctcc cgcaggctgg gcacggcggt
     4441 gatcaggcgt gtgaagatgt gacaattctc cttaaacttg gggaagttgt cctgcacgat
     4501 gcggtgtggg atggctgtgc tgatatcctc ggcgctgaac acgttcttcc tgttctcata
     4561 aaagccggag aagtaggttg taaacttgtc gaagctccgc agcagggcgt tctcgtgctc
     4621 ggttgtggtc acggtgccca gctgcttcag caccttgcca ttaaacagct cggccttgaa
     4681 caggcccttg tagatctcgg cgtgtctctt attgatggca tcggtcaggt tgtctgtccg
     4741 gccgatgaag tagtcgtgga tggcattgcg atatgtggcc tgctcctcga tcagggcgtt
     4801 ccttgtctcc tcggttttct cctttctata ggagtcgatg gcggcgctca ggttctccca
     4861 atccagctgc accagctgca ggcactggtc ggcataggtc ttgtagatcc gatcgatgat
     4921 gggcttcagc tccttgtagt gatcattgcg ggccttgtcc tcctcgatga agccctgctc
     4981 ctggatgtgc ttcagggtct tgccctgtgg gatcagctca aaccgcagtg tcttgctcac
     5041 ctgatacagg ttggtaaagc cctcgaactg tgtggctgct gggactccgt ggataccgac
     5101 cttccgcttc ttctttgggg ccatggtggc aagcttaagt ttaaacgcta gccagcttgg
     5161 gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt
     5221 agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     5281 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     5341 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     5401 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     5461 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     5521 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     5581 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     5641 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     5701 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     5761 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta
     5821 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
     5881 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
     5941 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
     6001 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca
     6061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6421 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6721 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7321 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7921 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7981 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8041 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8101 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8161 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8221 tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac
     8281 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     8341 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga
     8401 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     8461 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
     8521 cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     8581 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     8641 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     8701 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     8761 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     8821 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     8881 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     8941 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     9001 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     9061 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     9121 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
     9181 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     9241 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     9301 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     9361 tgtctcttga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     9421 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     9481 ggcctctgca taaataaaaa aaattagtc
//
