LOCUS       sequence-178241-        1854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of AT1R-APEX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, 
            Gygi SP, Kruse AC
  TITLE     Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed
            Proximity Labeling.
  JOURNAL   Cell. 2017 Apr 6;169(2):338-349.e11. doi: 
            10.1016/j.cell.2017.03.028.
  PUBMED    28388415
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-6- 6";
            volume: "169"; issue: "2"; pages: "338-349.e11"
COMMENT     Sequence Label: pcDNA5/FRT/TO-AT1R-APEX
FEATURES             Location/Qualifiers
     source          1..1854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1102..1848
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 atgattctca actcttctac tgaagatggt attaaaagaa tccaagatga ttgtcccaaa
       61 gctggaaggc ataattacat atttgtcatg attcctactt tatacagtat catctttgtg
      121 gtgggaatat ttggaaacag cttggtggtg atagtcattt acttttatat gaagctgaag
      181 actgtggcca gtgtttttct tttgaattta gcactggctg acttatgctt tttactgact
      241 ttgccactat gggctgtcta cacagctatg gaataccgct ggccctttgg caattaccta
      301 tgtaagattg cttcagccag cgtcagtttc aacctgtacg ctagtgtgtt tctactcacg
      361 tgtctcagca ttgatcgata cctggctatt gttcacccaa tgaagtcccg ccttcgacgc
      421 acaatgcttg tagccaaagt cacctgcatc atcatttggc tgctggcagg cttggccagt
      481 ttgccagcta taatccatcg aaatgtattt ttcattgaga acaccaatat tacagtttgt
      541 gctttccatt atgagtccca aaattcaacc ctcccgatag ggctgggcct gaccaaaaat
      601 atactgggtt tcctgtttcc ttttctgatc attcttacaa gttatactct tatttggaag
      661 gccctaaaga aggcttatga aattcagaag aacaaaccaa gaaatgatga tatttttaag
      721 ataattatgg caattgtgct tttctttttc ttttcctgga ttccccacca aatattcact
      781 tttctggatg tattgattca actaggcatc atacgtgact gtagaattgc agatattgtg
      841 gacacggcca tgcctatcac catttgtata gcttatttta acaattgcct gaatcctctt
      901 ttttatggct ttctggggaa aaaatttaaa agatattttc tccagcttct aaaatatatt
      961 cccccaaaag ccaaatccca ctcaaacctt tcaacaaaaa tgagcacgct ttcctaccgc
     1021 ccctcagata atgtaagctc atccaccaag aagcctgcac catgttttga ggttgagggt
     1081 ggaagttctg gcggttcaag tggaaagtct tacccaactg tgagtgctga ttaccaggac
     1141 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct
     1201 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccggt
     1261 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt
     1321 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc
     1381 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca
     1441 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcccgatccc
     1501 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa
     1561 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga
     1621 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg
     1681 ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct tttgtctgac
     1741 cctgtattcc gccctctcgt tgacaaatat gcagcggacg aagatgcctt ctttgctgat
     1801 tacgctgagg ctcaccaaaa gctttccgag cttgggtttg ctgatgccta atag
//
