LOCUS       sequence-178242-        2052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of B2AR-APEX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, 
            Gygi SP, Kruse AC
  TITLE     Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed
            Proximity Labeling.
  JOURNAL   Cell. 2017 Apr 6;169(2):338-349.e11. doi: 
            10.1016/j.cell.2017.03.028.
  PUBMED    28388415
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-6- 6";
            volume: "169"; issue: "2"; pages: "338-349.e11"
COMMENT     Sequence Label: pcDNA5/FRT/TO-B2AR-APEX
FEATURES             Location/Qualifiers
     source          1..2052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1264..2010
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             2011..2046
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 atggggcaac ctggcaatgg atcagctttt ctcctcgccc ctaatcggag ccacgctccc
       61 gaccacgatg tgactcagca gagagacgag gtctgggttg taggcatggg tattgtgatg
      121 tcactgatcg tcctggcaat tgtgttcggc aacgtcctcg tcattactgc tattgcaaag
      181 ttcgagcggc ttcagacggt taccaattac tttattactt ccctcgcctg tgccgacttg
      241 gtcatgggcc ttgcggttgt accgtttggc gcagctcata tcctgatgaa gatgtggact
      301 tttggtaatt tttggtgcga attctggact tcaattgacg tgctctgcgt cacagcgtca
      361 atcgaaactc tgtgtgttat tgccgttgat aggtacttcg cgattacgag tccctttaaa
      421 taccagtctc tgctgactaa gaacaaagct cgggttatca tactcatggt gtggatcgtc
      481 tcaggactca caagctttct gcctatacag atgcactggt atcgcgccac acaccaagaa
      541 gctatcaatt gttacgcaaa cgaaacctgt tgcgacttct tcactaatca agcctatgct
      601 atcgctagtt caattgtctc tttttatgtg cctctggtta tcatggtgtt cgtctactct
      661 cgggtttttc aggaggcaaa aagacagctc cagaaaatcg acaagtcaga aggccgattt
      721 cacgttcaaa atctgagcca ggtcgaacaa gacggaagaa ctggacatgg tcttcggaga
      781 tcttccaaat tttgcttgaa ggaacacaag gccctgaaga ccttggggat aattatgggc
      841 acattcactc tctgctggct cccctttttc attgtcaata tcgttcacgt tatacaagac
      901 aacttgatca gaaaagaggt gtatatcctc ctgaactgga ttggctacgt gaactctggg
      961 ttcaaccctc tgatttactg ccgcagccca gacttcagaa tcgcattcca ggaattgctg
     1021 tgtctgaggc gctcttcctt gaaggcttat ggaaacggat actcctctaa tggcaacacg
     1081 ggcgaacagt caggatacca cgtggagcag gaaaaagaga ataagctgtt gtgcgaggac
     1141 ctccctggca ctgaggattt tgttggccac caaggaacag tcccaagcga caatattgac
     1201 agccaggggc gaaactgcag cactaatgat tcactgctgg gtggaagttc tggcggttca
     1261 agtggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag
     1321 aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca
     1381 ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc
     1441 aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt
     1501 ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct
     1561 ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag
     1621 gacaagcctg agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat
     1681 ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct
     1741 gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc
     1801 tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa
     1861 ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc
     1921 gttgacaaat atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa
     1981 aagctttccg agcttgggtt tgctgatgcc gaagatcagg tggatccgcg cctgattgat
     2041 ggcaaataat ag
//
