LOCUS       sequence-178280-        8872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu 
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE
  TITLE     Optogenetic control with a photocleavable protein, PhoCl.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222.
  PUBMED    28288123
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222."
COMMENT     Sequence Label: pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
FEATURES             Location/Qualifiers
     source          1..8872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1742..1751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1751..2686
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2702..3427
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             3866..3886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3887..4120
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             4127..4852
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             4865..5800
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4913..5806
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5894..5913)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5959..6014
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6013..6032)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6375..6391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6375..6391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6388..6410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6399..6415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6423..6429,6430..6447,6448..6453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6468..6489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6548..6743
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6594..6729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6749..6883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6786..6805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6840..6859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6951..6968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7122..7710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7202..7221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7881..8672,8673..8741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASWQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYMTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8504..8523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8742..8846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg ggggggcgcg cgcgcggggg gggggggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ccgggtgagc
     1741 cgccaccatg gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa
     1801 acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt
     1861 gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga
     1921 agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa
     1981 ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct
     2041 agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc
     2101 agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga
     2161 gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa
     2221 tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta
     2281 cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct
     2341 ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca
     2401 gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa
     2461 caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct
     2521 gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc
     2581 cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt
     2641 gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctg tcgacggtac
     2701 cgtgatccct gactacttca agcagagctt ccccgagggc tacagctggg agcgcagcat
     2761 gacctacgag gacggcggca tctgcatcgc caccaacgac atcacaatgg agggggacag
     2821 cttcatcaac aagatccact tcaagggcac gaacttcccc cccaacggcc ccgtgatgca
     2881 gaagaggacc gtgggctggg aggccagcac cgagaagatg tacgagcgcg acggcgtgct
     2941 gaagggcgac gtgaagatga agctgctgct gaagggcggc ggccactatc gctgcgacta
     3001 ccgcaccacc tacaaggtca agcagaagcc cgtaaagctg cccgactacc acttcgtgga
     3061 ccaccgcatc gagatcctga gccacgacaa ggactacaac aaggtgaagc tgtacgagca
     3121 cgccgtggcc cgcaactcca ccgacagcat ggacgagctg tacaagggtg gcagcggtgg
     3181 catggtgagc aagggcgagg agaccattac aagcgtgatc aagcctgaca tgaagaacaa
     3241 gctgcgcatg gagggcaacg tgaacggcca cgccttcgtg atcgagggcg agggcagcgg
     3301 caagcccttc gagggcatcc agacgattga tttggaggtg aaggagggcg ccccgctgcc
     3361 cttcgcctac gacatcctga ccaccgcctt ccactacggc aaccgcgtgt tcaccaagta
     3421 cccacgggct agctctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg
     3481 ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc
     3541 tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct
     3601 agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt
     3661 gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa
     3721 tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac
     3781 attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca
     3841 aagacagttg actgtatcga tgggccctaa aaagaagcgt aaagtcgccc ccccgaccga
     3901 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga
     3961 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
     4021 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
     4081 gatgtttacc gatgcccttg gaattgacga gtacggtggg tccggagtga tccctgacta
     4141 cttcaagcag agcttccccg agggctacag ctgggagcgc agcatgacct acgaggacgg
     4201 cggcatctgc atcgccacca acgacatcac aatggagggg gacagcttca tcaacaagat
     4261 ccacttcaag ggcacgaact tcccccccaa cggccccgtg atgcagaaga ggaccgtggg
     4321 ctgggaggcc agcaccgaga agatgtacga gcgcgacggc gtgctgaagg gcgacgtgaa
     4381 gatgaagctg ctgctgaagg gcggcggcca ctatcgctgc gactaccgca ccacctacaa
     4441 ggtcaagcag aagcccgtaa agctgcccga ctaccacttc gtggaccacc gcatcgagat
     4501 cctgagccac gacaaggact acaacaaggt gaagctgtac gagcacgccg tggcccgcaa
     4561 ctccaccgac agcatggacg agctgtacaa gggtggcagc ggtggcatgg tgagcaaggg
     4621 cgaggagacc attacaagcg tgatcaagcc tgacatgaag aacaagctgc gcatggaggg
     4681 caacgtgaac ggccacgcct tcgtgatcga gggcgagggc agcggcaagc ccttcgaggg
     4741 catccagacg attgatttgg aggtgaagga gggcgccccg ctgcccttcg cctacgacat
     4801 cctgaccacc gccttccact acggcaaccg cgtgttcacc aagtacccac gggtcgagcc
     4861 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     4921 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     4981 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     5041 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     5101 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     5161 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     5221 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     5281 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     5341 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     5401 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     5461 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     5521 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     5581 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     5641 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     5701 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     5761 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatagc ggccgcactc
     5821 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     5881 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     5941 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     6001 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     6061 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     6121 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     6181 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     6241 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     6301 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     6361 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6541 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6601 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6661 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6721 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     6781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6841 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     6901 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7561 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7801 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7861 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7921 tgtctatttc gttcatccat agttgcctga ctccccgtca tgtagataac tacgatacgg
     7981 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8041 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8101 actttatccg cctccatcca gtctattaat tgttgccagg aagctagagt aagtagttcg
     8161 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8221 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8281 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8341 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8401 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8461 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8521 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8581 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8641 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8701 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
