LOCUS       sequence-178303-        6423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 841-1426.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP451
FEATURES             Location/Qualifiers
     source          1..6423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             618..641
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2447..3069
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2646..2665)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3093..3113)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3098..3427
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3278..3413
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3465..3484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3466..3484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3498..3514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3498..3514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3511..3533)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3522..3538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3546..3552,3553..3570,3571..3576))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3591..3612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3729..3746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3900..4488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3980..3999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4659..5450,5451..5519))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5282..5301
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5520..5624)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5651..6106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5738..5757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5948..5969
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6232..6254
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6246..6263
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6247..6263
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc gcaacattgc
      661 caccgtccag atctcgggtc atctcaaacg atggagccag ccgcttctct ggaggcagcc
      721 tcctcacgac tggaagctca aggcgaaaac acgtggtgca agcacagaag ctggcggacg
      781 tggacagtga gctggctgcc atgctactga cccatgcccg gcagggcaag gggccccagg
      841 acgtcagccg cgagtcggat gccacccgca ggcgtaagct ggagcggatg aggtctgtgc
      901 gcctgcagga ggccggggga gacttgggcc ggcgtgggac gagcgtgttg gcgcagcaga
      961 gcgtccgcac acagcacttg cgggacctac aggtcatcgc cgcctaccgg gaacgcacga
     1021 aggccgagag catcgccagc ctgctgagcc tggccatcac cacggagcac acgctccacg
     1081 ccacgctggg ggtcgccgag ttctttgagt ttgtgcttaa gaacccccac aacacacagc
     1141 acacggtgac tgtggagatc gacaaccccg agctcagcgt catcgtggac agtcaggagt
     1201 ggagggactt caagggtgct gctggcctgc acacaccggt ggaggaggac atgttccacc
     1261 tgcgtggcag cctggccccc cagctctacc tgcgccccca cgagaccgcc cacgtcccct
     1321 tcaagttcca gagcttctct gcagggcagc tggccatggt gcaggcctct cctgggttga
     1381 gcaacgagaa gggcatggac gccgtgtcac cttggaagtc cagcgcagtg cccactaaac
     1441 acgccaaggt cttgttccga gcgagtggtg gcaagcccat cgccgtgctc tgcctgactg
     1501 tggagctgca gccccacgtg gtggaccagg tcttccgctt ctatcacccg gagctctcct
     1561 tcctgaagaa ggccatccgc ctgccgccct ggcacacatt tccaggtgct ccggtgggaa
     1621 tgcttggtga ggacccccca gtccatgttc gctgcagcga cccgaacgtc atctgtgaga
     1681 cccagaatgt gggccccggg gaaccacggg acatatttct gaaggtggcc agtggtccaa
     1741 gcccggagat caaagacttc tttgtcatca tttactcgga tcgttggctg gcgacaccca
     1801 cacagacgtg gcaggtctac ctccactccc tgcagcgcgt ggatgtctcc tgcgtcgcag
     1861 gccagctgac ccgcctgtcc cttgtccttc gggggacaca gacagtgagg aaagtgagag
     1921 ctttcacctc tcatccacag gagctgaaga cagaccccaa aggtgtcttc gtgctgccgc
     1981 ctcgtggggt gcaggacctg catgttggcg tgaggcccct tagggccggc agccgctttg
     2041 tccatctcaa cctggtggac gtggattgcc accagctggt ggcctcctgg ctcgtgtgcc
     2101 tctgctgccg ccagccgctc atctccaagg cctttgagat catgttggct gcgggcgaag
     2161 ggaagggtgt caacaagagg atcacctaca ccaaccccta cccctcccgg aggacattcc
     2221 acctgcacag cgaccacccg gagctgctgc ggttcagaga ggactccttc caggtcgggg
     2281 gtggagagac ctacaccatc ggcttgcagt ttgcgcctag tcagagagtg ggtgaggagg
     2341 agatcctgat ctacatcaat gaccatgagg acaaaaacga agaggcattt tgcgtgaagg
     2401 tcatctacca gtgagatatc ggtaccagtc gactctagag gatcccgggt ggcatccctg
     2461 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
     2521 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
     2581 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
     2641 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
     2701 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
     2761 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
     2821 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
     2881 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact
     2941 ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg
     3001 acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc
     3061 tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag ggtgtggaaa
     3121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3181 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3241 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3361 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3421 ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aattccctat agtgagtcgt
     3481 attaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5641 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5701 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5761 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5821 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5881 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5941 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6001 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6061 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca
     6121 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     6181 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     6241 gacgttgtaa aacgacggcc agtgccaagc tgatctatac attgaatcaa tattggcaat
     6301 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     6361 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
     6421 gtt
//
