LOCUS       sequence-178307-        4556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Schou KB, Morthorst SK, Christensen ST, Pedersen LB
  TITLE     Identification of conserved, centrosome-targeting ASH domains in 
            TRAPPII complex subunits and TRAPPC8.
  JOURNAL   Cilia. 2014 Jun 18;3:6. doi: 10.1186/2046-2530-3-6. eCollection 
            2014.
  PUBMED    25018876
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cilia."; 
            date: "2014-06-18"; volume: "3:6. doi"; pages: " 
            10.1186/2046-2530-3-6. eCollection 2014"
COMMENT     Sequence Label: LP376
FEATURES             Location/Qualifiers
     source          1..4556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1669..1803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1693..1712)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1747..1766
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1914..1930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1914..1930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1927..1949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1938..1954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1962..1968,1969..1986,1987..1992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2007..2028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2145..2162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2316..2904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2396..2415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3075..3866,3867..3935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3698..3717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3936..4040)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4108..4126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4164..4186)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4286..4305
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4499..4521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4513..4530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4514..4530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatggcatc aatgcagaag ctgatctcag aggaggacct
      841 gcttatggcc atggaggccc gaattctccc catctacagc aagcagtcgg tgttcttcgt
      901 ctgggaactc aagtggacag aagagcctcc cccttctctg cattgccggt tctctgttgg
      961 attttcccca gcttctgagg aacagctgtc tatctcctta aagccgtata cttatgaatt
     1021 taaagtggaa aattttttta cattatacaa cgtgaaggct gagatctttc ccccttcggg
     1081 aatggagtat tgcagaacag gctccctctg ctccctggag gttttgatca cgaggctctc
     1141 agacctcttg gaggtggata aagatgaagc actgactgaa tctgatgagc atttttcgac
     1201 aaagcttatg tatgaagttg tcgacaacag tagcaactgg gcagtgtgtg ggaaaagctg
     1261 cggtgtcatc tccatgccag tggctgctcg ggccactcac agggtccaca tggaagtgat
     1321 gccgctcttc gccgggtatc tccccctgcc cgacgtcagg ctgttcaagt acctccccca
     1381 tcattctgca cactcctccc aactggacgc tgacagctgg atagaaaacg acagcctgtc
     1441 agtagacaag cacggggacg accagccgga cagcagcagc ctcaagagca ggggcagcgt
     1501 gcattcggcc tgcagcagcg agcacaaagg cctacccatg ccccggctgc aggcactgcc
     1561 ggccggccag gtcttcaact ccagctcggg cacacaagtc ctggtcatcc ccagccaaga
     1621 tgaccacgtc ctggaagtca gtgtaacatg aggtaccgcg gccgcgggga tccagacatg
     1681 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1741 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1801 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1861 ttttcggatc ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag
     1921 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1981 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4141 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4201 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4261 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4321 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     4381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     4501 cagtcacgac gttgtaaaac gacggccagt gagttcgagc ttgcatgcct gcaggt
//
