LOCUS       sequence-178310-        8087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc with cDNA encoding HsTRAPPC8 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Verdier P, Morthorst SK, Pedersen LB
  TITLE     Targeting of ASH Domain-Containing Proteins to the Centrosome.
  JOURNAL   Methods Mol Biol. 2016;1454:15-33. doi: 10.1007/978-1-4939-3789-9_2.
  PUBMED    27514913
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1454:15-33. doi"; pages: " 
            10.1007/978-1-4939-3789-9_2"
COMMENT     Sequence Label: LP538
FEATURES             Location/Qualifiers
     source          1..8087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    5200..5334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5224..5243)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5278..5297
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5445..5461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5445..5461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5458..5480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5469..5485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5493..5499,5500..5517,5518..5523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5538..5559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5676..5693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5847..6435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5927..5946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6606..7397,7398..7466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7229..7248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7467..7571)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7639..7657
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7695..7717)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7817..7836
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8030..8052
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8044..8061
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8045..8061
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatggcatc aatgcagaag ctgatctcag aggaggacct
      841 gcttatggcc atggaggccc gaattcggtc gactatggcc cagtgtgtac aatcagtgca
      901 ggagctaatc ccggactcct tcgtcccctg tgtcgctgcg ctgtgcagcg acgaagccga
      961 gcggctcact cgtctcaatc acctcagctt cgcggagctg cttaagccct tctcccgcct
     1021 cacttccgag gttcacatga gagatcctaa taatcaactt cacgtaatta aaaatttgaa
     1081 gatagcagta agcaacattg tcacccagcc acctcagcct ggagccatcc ggaagctttt
     1141 gaatgatgtt gtttctggca gtcagcctgc agaaggatta gtagctaatg tgattacagc
     1201 aggagattat gaccttaaca tcagtgccac tactccatgg tttgagtctt acagagaaac
     1261 ctttcttcag tcgatgccag catcggatca tgaatttctg aaccactatt tagcatgtat
     1321 gttggtagcg tcatctagtg aagctgaacc tgtggaacag ttttcaaagt tgtcacaaga
     1381 acagcatcga attcagcaca acagtgatta ttcctacccc aagtggttta taccaaatac
     1441 acttaaatac tatgtacttt tacatgatgt aagtgcagga gatgaacaga gagctgaatc
     1501 aatttatgaa gaaatgaaac agaaatatgg aactcagggt tgctatttac ttaaaattaa
     1561 ttctcgaaca tctaatcgag catcagatga acagatacca gatccttgga gtcagtatct
     1621 ccagaaaaat agtattcaaa accaggaatc atatgaagat ggcccttgta ctataacttc
     1681 aaataagaat tctgataata acttgctttc attggatgga ttagataacg aagtcaaaga
     1741 tggcttacca aataacttta gagctcaccc acttcagttg gagcaatcca gtgacccttc
     1801 taacagtatt gatggcccag atcatctaag atctgcttca tcgttacatg aaacaaagaa
     1861 aggaaatact ggaataattc atggtgcatg tttaacactt actgatcatg atagaattcg
     1921 acagtttata caagagttca catttcgggg ccttttgcca catatagaga aaacaattag
     1981 gcaattaaac gatcagctaa tatcaagaaa aggtttgagt cgatctctat tttctgcaac
     2041 taaaaaatgg tttagtggca gtaaagttcc agaaaagagc attaatgacc tgaaaaatac
     2101 atctggcttg ctgtatccgc cggaagcacc agaacttcaa atcaggaaaa tggctgactt
     2161 atgttttttg gtgcagcatt atgatttggc ttacagttgc tatcatactg caaagaaaga
     2221 ttttcttaat gatcaagcaa tgctttatgc agctggtgcc ttggaaatgg cagcagtgtc
     2281 tgcttttctt caaccaggag cacctaggcc atatcctgct cattacatgg atacagcaat
     2341 tcagacatac agagatatct gcaagaatat ggtgttggct gaaagatgtg tgttgcttag
     2401 tgctgaactt ttaaaaagcc aaagcaaata ttcagaggct gcagctctcc taatacggtt
     2461 gaccagtgag gattctgatc ttcgaagtgc acttcttttg gaacaggcag cacattgctt
     2521 tataaacatg aaaagtccca tggttagaaa atatgcattt catatgatat tggcaggcca
     2581 tcgatttagt aaagcagggc agaaaaagca tgctttacgc tgttattgtc aagccatgca
     2641 agtttacaaa ggaaaaggct ggtctcttgc agaggatcac attaatttca ctattgggcg
     2701 ccagtcctat actcttagac agctggataa tgctgtgtct gcttttaggc atattctaat
     2761 taatgaaagt aaacaatctg ctgctcaaca gggggctttc ctcagagaat atctttatgt
     2821 ttacaagaat gtaagtcagc tgtcaccaga tggtcctttg ccacagcttc ctttaccgta
     2881 tattaacagt tcagcaacac gggttttttt tggccatgac agacgaccag cggatggtga
     2941 aaaacaagca gctactcatg taagtcttga tcaagaatat gattctgaat cctctcagca
     3001 gtggcgagaa cttgaggaac aagttgtttc tgtggttaac aaaggagtaa ttccatccaa
     3061 ttttcatccc acacaatact gtttgaacag ttactcagat aattcaagat ttccacttgc
     3121 agttgtagaa gaaccaatta cagtggaagt ggcttttaga aaccctttga aagttctact
     3181 tttgttgact gatttgtcat tgctttggaa gtttcatcct aaagatttca gtggaaagga
     3241 taatgaagaa gttaaacaac tagttacaag tgaacctgaa atgattggag ctgaagttat
     3301 ttcagagttc ttaattaatg gcgaagaatc aaaagtggca agactaaagc tctttcccca
     3361 tcacataggg gagctgcata ttctgggagt tgtttataat cttggcacta ttcagggctc
     3421 tatgacagta gatggcattg gtgctcttcc cggatgtcac acaggaaaat attccttgag
     3481 tatgtcagtc cgagggaagc aggatttaga aattcaaggt cctcgactta acaacacaaa
     3541 agaagagaaa acatctgtta aatatggccc tgatcgacgt ttagatccca taatcacaga
     3601 agaaatgcca ctgttggagg tgttctttat acattttcct acagggcttc tctgtggaga
     3661 aatccgaaaa gcatatgtag aatttgtcaa tgtcagcaaa tgtccactta ctggattgaa
     3721 ggttgtttct aaacgtccag agttctttac tttcggtggt aatactgctg ttctaacacc
     3781 actaagtccc tcagcttctg agaattgtag tgcttacaag actgttgtga cagatgctac
     3841 ctctgtgtgt acagcactca tatcatcagc ttcttctgta gactttggca ttggcacagg
     3901 aagtcaacca gaggtgattc ctgttcccct tcctgacact gttcttctac ccggagcctc
     3961 agtgcagctg ccaatgtggt tacgtgggcc tgatgaagaa ggtgtccatg aaattaactt
     4021 tttgttttac tatgaaagtg tcaaaaagca gccaaaaata cggcacagaa tattaagaca
     4081 cactgcaatt atttgtacca gtcggtcttt aaatgtacgg gccactgtct gcagaagtaa
     4141 ttctcttgaa aatgaagaag gcagaggagg caatatgcta gtctttgtgg atgtggaaaa
     4201 taccaatact agtgaagcag gcgttaagga attccacata gtgcaagtat caagtagtag
     4261 caaacactgg aagttacaga aatctgtaaa tctttctgaa aacaaagatg ccaaacttgc
     4321 cagtagggag aagggaaagt tttgctttaa ggcaataaga tgtgagaaag aagaagcggc
     4381 cacacagtcc tctgaaaaat atacctttgc agatatcatc tttggaaatg aacagataat
     4441 aagttcagca agcccatgtg cagacttctt ttatcgaagt ttatcttctg aattgaaaaa
     4501 accacaagct cacttgcctg tgcatacaga aaaacagtca acagaggatg ctgtgagatt
     4561 gattcaaaaa tgcagtgagg tagatttgaa tattgtcata ttatggaagg catacgttgt
     4621 ggaagacagt aaacagctta ttttggaagg tcaacatcat gttattcttc gcactatagg
     4681 aaaagaagcc ttttcatatc ctcagaaaca ggagccacca gaaatggaac tattgaaatt
     4741 tttcaggcca gaaaacatta cagtttcctc aaggccatca gtagagcagc tttctagtct
     4801 cattaaaacg agtcttcact acccagaatc atttaatcat ccatttcatc aaaaaagcct
     4861 ttgtttagta ccagtcactc ttttactttc caattgttct aaggctgatg tagatgtcat
     4921 agttgatctt cggcataaaa caacaagtcc agaagcactg gaaatccatg gatcattcac
     4981 atggcttgga caaacacagt ataaacttca acttaaaagc caggagattc acagtctgca
     5041 gctgaaagca tgctttgttc atacaggtgt ttataacctt ggaactccta gggtatttgc
     5101 caagttatcg gaccaagtta cagtgtttga aacaagtcag cagaattcca tgcctgccct
     5161 gatcatcatc agtaatgtgt gaggtaccgc ggccgcgggg atccagacat gataagatac
     5221 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5281 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5341 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat
     5401 cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct
     5461 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5521 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5581 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5641 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6901 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7201 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7621 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     7681 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7741 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7801 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7861 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     7921 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7981 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8041 cgttgtaaaa cgacggccag tgagttcgag cttgcatgcc tgcaggt
//
