LOCUS       sequence-178313-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc with cDNA encoding HsTRAPPC8 aa residues 1-700.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Verdier P, Morthorst SK, Pedersen LB
  TITLE     Targeting of ASH Domain-Containing Proteins to the Centrosome.
  JOURNAL   Methods Mol Biol. 2016;1454:15-33. doi: 10.1007/978-1-4939-3789-9_2.
  PUBMED    27514913
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1454:15-33. doi"; pages: " 
            10.1007/978-1-4939-3789-9_2"
COMMENT     Sequence Label: LP539
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2995..3129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3019..3038)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3073..3092
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3240..3256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3240..3256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3253..3275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3264..3280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3288..3294,3295..3312,3313..3318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3333..3354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3471..3488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3642..4230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3722..3741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4401..5192,5193..5261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5024..5043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5262..5366)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5434..5452
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5490..5512)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5612..5631
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5825..5847
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5839..5856
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5840..5856
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatggcatc aatgcagaag ctgatctcag aggaggacct
      841 gcttatggcc atggaggccc gaattcggtc gactatggcc cagtgtgtac aatcagtgca
      901 ggagctaatc ccggactcct tcgtcccctg tgtcgctgcg ctgtgcagcg acgaagccga
      961 gcggctcact cgtctcaatc acctcagctt cgcggagctg cttaagccct tctcccgcct
     1021 cacttccgag gttcacatga gagatcctaa taatcaactt cacgtaatta aaaatttgaa
     1081 gatagcagta agcaacattg tcacccagcc acctcagcct ggagccatcc ggaagctttt
     1141 gaatgatgtt gtttctggca gtcagcctgc agaaggatta gtagctaatg tgattacagc
     1201 aggagattat gaccttaaca tcagtgccac tactccatgg tttgagtctt acagagaaac
     1261 ctttcttcag tcgatgccag catcggatca tgaatttctg aaccactatt tagcatgtat
     1321 gttggtagcg tcatctagtg aagctgaacc tgtggaacag ttttcaaagt tgtcacaaga
     1381 acagcatcga attcagcaca acagtgatta ttcctacccc aagtggttta taccaaatac
     1441 acttaaatac tatgtacttt tacatgatgt aagtgcagga gatgaacaga gagctgaatc
     1501 aatttatgaa gaaatgaaac agaaatatgg aactcagggt tgctatttac ttaaaattaa
     1561 ttctcgaaca tctaatcgag catcagatga acagatacca gatccttgga gtcagtatct
     1621 ccagaaaaat agtattcaaa accaggaatc atatgaagat ggcccttgta ctataacttc
     1681 aaataagaat tctgataata acttgctttc attggatgga ttagataacg aagtcaaaga
     1741 tggcttacca aataacttta gagctcaccc acttcagttg gagcaatcca gtgacccttc
     1801 taacagtatt gatggcccag atcatctaag atctgcttca tcgttacatg aaacaaagaa
     1861 aggaaatact ggaataattc atggtgcatg tttaacactt actgatcacg atagaattcg
     1921 acagtttata caagagttca catttcgggg ccttttgcca catatagaga aaacaattag
     1981 gcaattaaac gatcagctaa tatcaagaaa aggtttgagt cgatctctat tttctgcaac
     2041 taaaaaatgg tttagtggca gtaaagttcc agaaaagagc attaatgacc tgaaaaatac
     2101 atctggcttg ctgtatccgc cggaagcacc agaacttcaa atcaggaaaa tggctgactt
     2161 atgttttttg gtgcagcatt atgatttggc ttacagttgc tatcatactg caaagaaaga
     2221 ttttcttaat gatcaagcaa tgctttatgc agctggtgcc ttggaaatgg cagcagtgtc
     2281 tgcttttctt caaccaggag cacctaggcc atatcctgct cattacatgg atacagcaat
     2341 tcagacatac agagatatct gcaagaatat ggtgttggct gaaagatgtg tgttgcttag
     2401 tgctgaactt ttaaaaagcc aaagcaaata ttcagaggct gcagctctcc taatacggtt
     2461 gaccagtgag gattctgatc ttcgaagtgc acttcttttg gaacaggcag cacattgctt
     2521 tataaacatg aaaagtccca tggttagaaa atatgcattt catatgatat tggcaggcca
     2581 tcgatttagt aaagcagggc agaaaaagca tgctttacgc tgttactgtc aagccatgca
     2641 agtttacaaa ggaaaaggct ggtctcttgc agaggatcac attaatttca ctattgggcg
     2701 ccagtcctat actcttagac agctggataa tgctgtgtct gcttttaggc atattctaat
     2761 taatgaaagt aaacaatctg ctgctcaaca gggggctttc ctcagagaat atctttatgt
     2821 ttacaagaat gtaagtcagc tgtcaccaga tggtcctttg ccacagcttc ctttaccgta
     2881 tattaacagt tcagcaacac gggttttttt tggccatgac agacgaccag cggatggtga
     2941 aaaacaagca gctactcatg tagatcttga tcaatgaggt accgcggccg cggggatcca
     3001 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3061 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3121 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3181 gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc gtaatcatgg
     3241 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3361 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3421 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4381 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4441 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4501 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4561 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4621 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4681 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4741 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4801 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4861 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4921 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4981 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5041 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5101 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5161 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5221 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5281 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5341 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5401 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5461 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     5521 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     5581 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     5641 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     5701 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5761 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5821 ttttcccagt cacgacgttg taaaacgacg gccagtgagt tcgagcttgc atgcctgcag
     5881 gt
//
