LOCUS       sequence-178314-        5987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc cDNA encoding HsTRAPPC8 aa residues 701-1436.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Verdier P, Morthorst SK, Pedersen LB
  TITLE     Targeting of ASH Domain-Containing Proteins to the Centrosome.
  JOURNAL   Methods Mol Biol. 2016;1454:15-33. doi: 10.1007/978-1-4939-3789-9_2.
  PUBMED    27514913
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1454:15-33. doi"; pages: " 
            10.1007/978-1-4939-3789-9_2"
COMMENT     Sequence Label: LP555
FEATURES             Location/Qualifiers
     source          1..5987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3100..3234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3124..3143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3178..3197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3345..3361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3345..3361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3358..3380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3369..3385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3393..3399,3400..3417,3418..3423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3438..3459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3576..3593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3747..4335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3827..3846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4506..5297,5298..5366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5129..5148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5367..5471)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5539..5557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5595..5617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5717..5736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5930..5952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5944..5961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5945..5961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatggcatc aatgcagaag ctgatctcag aggaggacct
      841 gcttatggcc atggaggccc gaattcggtc gactgaatat gattctgaat cctctcagca
      901 gtggcgagaa cttgaggaac aagttgtttc tgtggttaac aaaggagtaa ttccatccaa
      961 ttttcatccc acacaatact gtttgaacag ttactcagat aattcaagat ttccacttgc
     1021 agttgtagaa gaaccaatta cagtggaagt ggcttttaga aaccctttga aagttctact
     1081 tttgttgact gatttgtcat tgctttggaa gtttcatcct aaagatttca gtggaaagga
     1141 taatgaagaa gttaaacaac tagttacaag tgaacctgaa atgattggag ctgaagttat
     1201 ttcagagttc ttaattaatg gcgaagaatc aaaagtggca agactaaagc tctttcccca
     1261 tcacataggg gagctgcata ttctgggagt tgtttataat cttggcacta ttcagggctc
     1321 tatgacagta gatggcattg gtgctcttcc cggatgtcac acaggaaaat attccttgag
     1381 tatgtcagtc cgagggaagc aggatttaga aattcaaggt cctcgactta acaacacaaa
     1441 agaagagaaa acatctgtta aatatggccc tgatcgacgt ttagatccca taatcacaga
     1501 agaaatgcca ctgttggagg tgttctttat acattttcct acagggcttc tctgtggaga
     1561 aatccgaaaa gcatatgtag aatttgtcaa tgtcagcaaa tgtccactta ctggattgaa
     1621 ggttgtttct aaacgtccag agttctttac tttcggtggt aatactgctg ttctaacacc
     1681 actaagtccc tcagcttctg agaattgtag tgcttacaag actgttgtga cagatgctac
     1741 ctctgtgtgt acagcactca tatcatcagc ttcttctgta gactttggca ttggcacagg
     1801 aagtcaacca gaggtgattc ctgttcccct tcctgacact gttcttctac ccggagcctc
     1861 agtgcagctg ccaatgtggt tacgtgggcc tgatgaagaa ggtgtccatg aaattaactt
     1921 tttgttttac tatgaaagtg tcaaaaagca gccaaaaata cggcacagaa tattaagaca
     1981 cactgcaatt atttgtacca gtcggtcttt aaatgtacgg gccactgtct gcagaagtaa
     2041 ttctcttgaa aatgaagaag gcagaggagg caatatgcta gtctttgtgg atgtggaaaa
     2101 taccaatact agtgaagcag gcgttaagga attccacata gtgcaagtat caagtagtag
     2161 caaacactgg aagttacaga aatctgtaaa tctttctgaa aacaaagatg ccaaacttgc
     2221 cagtagggag aagggaaagt tttgctttaa ggcaataaga tgtgagaaag aagaagcggc
     2281 cacacagtcc tctgaaaaat atacctttgc agatatcatc tttggaaatg aacagataat
     2341 aagttcagca agcccatgtg cagacttctt ttatcgaagt ttatcttctg aattgaaaaa
     2401 accacaagct cacttgcctg tgcatacaga aaaacagtca acagaggatg ctgtgagatt
     2461 gattcaaaaa tgcagtgagg tagatttgaa tattgtcata ttatggaagg catacgttgt
     2521 ggaagacagt aaacagctta ttttggaagg tcaacatcat gttattcttc gcactatagg
     2581 aaaagaagcc ttttcatatc ctcagaaaca ggagccacca gaaatggaac tattgaaatt
     2641 tttcaggcca gaaaacatta cagtttcctc aaggccatca gtagagcagc tttctagtct
     2701 cattaaaacg agtcttcact acccagaatc atttaatcat ccatttcatc aaaaaagcct
     2761 ttgtttagta ccagtcactc ttttactttc caattgttct aaggctgatg tagatgtcat
     2821 agttgatctt cggcataaaa caacaagtcc agaagcactg gaaatccatg gatcattcac
     2881 atggcttgga caaacacagt ataaacttca acttaaaagc caggagattc acagtctgca
     2941 gctgaaagca tgctttgttc atacaggtgt ttataacctt ggaactccta gggtatttgc
     3001 caagttatcg gaccaagtta cagtgtttga aacaagtcag cagaattcca tgcctgccct
     3061 gatcatcatc agtaatgtgt gaggtaccgc ggccgcgggg atccagacat gataagatac
     3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3241 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat
     3301 cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct
     3361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     5581 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5641 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5701 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     5761 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     5821 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5881 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     5941 cgttgtaaaa cgacggccag tgagttcgag cttgcatgcc tgcaggt
//
