LOCUS       sequence-178315-        5027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc with cDNA encoding HsTRAPPC13 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Verdier P, Morthorst SK, Pedersen LB
  TITLE     Targeting of ASH Domain-Containing Proteins to the Centrosome.
  JOURNAL   Methods Mol Biol. 2016;1454:15-33. doi: 10.1007/978-1-4939-3789-9_2.
  PUBMED    27514913
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1454:15-33. doi"; pages: " 
            10.1007/978-1-4939-3789-9_2"
COMMENT     Sequence Label: LP580
FEATURES             Location/Qualifiers
     source          1..5027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          342..438
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1836..1970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1860..1879)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1914..1933
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2081..2097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2081..2097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2094..2116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2105..2121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2129..2135,2136..2153,2154..2159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2174..2195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2312..2329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2483..3071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2563..2582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3242..4033,4034..4102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3865..3884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4103..4207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4275..4293
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4331..4353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4453..4472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4666..4688
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4680..4697
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4681..4697
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4724..5027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagag
      301 gatccggtac tagaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
      361 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga
      421 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat
      481 tgtacccgcg ggcccaccat ggcatcaatg cagaagctga tctcagagga ggacctgctt
      541 atggccatgg aggcccgaat tcttatggaa gtgaatcccc ctaaacagga gcacctgctg
      601 gcgctaaaag tgatgcggct gactaagcct actttattca ccaatatccc agtaacatgt
      661 gaagagaaag acttacctgg agatctcttt aaccagctga tgagagatga tccttcaacc
      721 gttaatggtg cagaagtttt aatgttggga gaaatgctga ctttaccaca gaattttggg
      781 aatatatttt tgggagagac cttttccagt tatatcagcg ttcataatga tagcaatcaa
      841 gttgtaaaag acatattagt aaaagctgat cttcagacaa gttctcagcg tttaaatctt
      901 tcagcctcca atgctgcagt ggctgaactt aaaccggatt gttgtattga tgatgtcata
      961 catcatgaag tcaaagaaat tggaacacac atcttggtat gtgctgtgag ttatacaact
     1021 caggctggag aaaaaatgta tttcagaaaa ttcttcaaat ttcaggttct caaaccattg
     1081 gatgtgaaaa ccaaatttta caatgcagag agtgacctca gttctgtgac tgatgaagta
     1141 tttctggaag cccagattca gaatatgaca acctcaccta tgtttatgga gaaggtttca
     1201 ctggagccat ctattatgta caatgtaaca gaattaaatt cagtcagcca agctggagaa
     1261 tgtgtgtcta cgtttgggtc aagagcatat ttgcaaccaa tggatacacg ccagtactta
     1321 tactgcctaa agccaaagaa tgaatttgca gaaaaagcag gcatcattaa gggagtaaca
     1381 gtaattggaa aattggatat agtatggaaa acaaatctag gtgaaagggg aaggttacag
     1441 accagccaac ttcaaagaat ggctccaggt tatggagatg ttaggttgtc tttggaggca
     1501 ataccagata ccgtaaacct tgaagaacct tttcatatta cctgtaaaat aacaaactgc
     1561 agtgaaagga ctatggatct ggttttggaa atgtgcaata ccaattccat ccactggtgt
     1621 ggaatttcag gaagacagct gggaaagctg catccaagtt cttcgctctg tcttgccctt
     1681 actctgcttt cttcagtaca gggactgcaa agcatctctg gcttaagact aacagacaca
     1741 ttcttaaaga gaacatatga atatgatgac atcgcacaag tctgtgtggt atcttctgcc
     1801 attaaagtgg aaagctgagg taccgcggcc gcggggatcc agacatgata agatacattg
     1861 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1921 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1981 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc
     2041 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4201 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4261 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4321 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4501 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     4561 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4621 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     4681 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg
     4741 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     4801 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4861 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4921 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4981 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
