LOCUS       sequence-178327-        4049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ThsS constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Daeffler KN, Galley JD, Sheth RU, Ortiz-Velez LC, Bibb CO, Shroyer 
            NF, Britton RA, Tabor JJ
  TITLE     Engineering bacterial thiosulfate and tetrathionate sensors for 
            detecting gut inflammation.
  JOURNAL   Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416.
  PUBMED    28373240
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2017-04-3"; volume: "13(4):923. doi"; pages: " 
            10.15252/msb.20167416"
COMMENT     Sequence Label: pKD236-4b
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             149..940
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      952..1046
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1160..1705
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1606..1625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1822..1839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2014..2048
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      3884..3955
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3971..3998
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
       61 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
      121 tcaagagaca ggatgaggat cgtttcgtat gagggaagcg gtgatcgccg aagtatcgac
      181 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      241 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      301 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      361 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      421 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      481 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      541 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      601 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      661 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      721 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      781 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
      841 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
      901 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa gagctcgctt ggactcctgt
      961 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1021 gccgggcgtt ttttattggt gagaatccaa gcactagtaa caacttatat cgtatggggc
     1081 tgacttcagg tgctacattt gaagagataa attgcactga aatctagtaa tattttatct
     1141 gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa
     1201 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga
     1261 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt
     1321 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt
     1381 ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     1441 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     1501 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt
     1561 aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta
     1621 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     1681 gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta
     1741 agtatcttcc tggcatcttc cgggaaatct ccgccccgtt cgtaagccat ttccgctcgc
     1801 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcccctag
     1861 gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgt aaatcactgc
     1921 ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca
     1981 taacggttct ggcaaatatt ctgaaatgag ctgttgacag ctagctcagt cctaggtatt
     2041 gtgctagcct agtatcgatc tccataacta tcctatagat catgtcccgc ctgctgctgt
     2101 gtatctgtgt tctgctgttc tcttctgtgg cgtggtctaa accgcagcag ttttatgtgg
     2161 gcgtactggc taactggggt catcagcaag ccgttgaacg ttggaccccg atgatggagt
     2221 atctgaacga acatgtgccg gacgcggaat ttcacgtcta cccgggcaac ttcaaagcac
     2281 tgaacctggc aatggaactg ggccagattc agttcattat cactaacccg ggccaatatc
     2341 tgtacctgag caatcagtac ccgctgtctt ggctggcgac catgcgttct aagcgtcacg
     2401 atggtaccac ttctgcgatc ggttccgcca ttattgtccg cgcggacagc gactaccgca
     2461 ccctgtacga cctgaaaggt aaagtggtgg ctgcgtccga cccgcatgct ctgggtggct
     2521 accaagcgac cgtcggtctg atgcattccc tgggcatgga tccggacacc ttcttcggtg
     2581 aaaccaagtt tctgggcttt ccactggatc cgctgctgta ccaagttcgt gatggcaacg
     2641 ttgacgcggc cattacccca ctgtgcactc tggaggacat ggttgcacgc ggcgtactga
     2701 aatcttccga ttttcgtgtg ctgaacccta gccgcccgga tggtgtagaa tgccagtgct
     2761 ctaccaccct gtacccgaac tggtctttcg ctgcgactga gtctgtatcc accgaactgt
     2821 ctaaagaaat cacgcaggca ctgctggaac tgccatccga cagcccggca gctatcaaag
     2881 cgcaactgac cggctggacc agcccgatct cccaactggc ggtaatcaaa ctgttcaaag
     2941 agctgcacgt aaaaaccccg gactctagcc gttgggaagc cgttaagaag tggctggaag
     3001 aaaaccgtca ctggggtatc ctgtctgttc tggtgttcat cattgcaacg ctgtatcacc
     3061 tgtggattga ataccgcttc caccaaaaaa gctcttctct gatcgaatct gaacgtcagc
     3121 tgaaacagca agctgttgcc ctggaacgtc tgcaatctgc tagcatcgtt ggtgaaattg
     3181 gtgcgggtct ggcccacgag attaatcagc cgatcgctgc aattacctct tattctgaag
     3241 gtggcatcat gcgcctgcaa ggtaaagaac aggcggatac ggatagctgc atcgaactgc
     3301 tggaaaaaat ccacaaacag agcactcgcg caggcgaagt ggtgcaccgc atccgtggtc
     3361 tgctgaaacg tcgtgaagcg gtgatggtag atgttaacat cctgaccctg gtggaagaat
     3421 ccatcagcct gctgcgtctg gagctggcac gtcgcgaaat ccagatcaac actcagatca
     3481 aaggtgaacc gttcttcatt actgccgacc gcgttggcct gctgcaagtt ctgattaacc
     3541 tgatcaaaaa ctccctggac gcgatcgctg aatctgataa tgcccgttct ggtaaaatca
     3601 acatcgaact ggactttaaa gagtaccagg taaacgtctc catcatcgat aacggtccgg
     3661 gcctggcgat ggattctgac actctgatgg ctacgtttta cactaccaaa atggatggcc
     3721 tgggcctggg tctggcaatc tgccgcgaag ttatcagcaa ccacgacggc cacttcctgc
     3781 tgtccaaccg tgacgacggc gttctgggct gtgtggcaac cctgaatctg aaaaaacgcg
     3841 gttctgaagt gccgatcgaa gtctaataat ctagaccagg catcaaataa aacgaaaggc
     3901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     3961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tataggtacc cggggatcct
     4021 ctagagtcga cctgcaggca tgcaagctt
//
