LOCUS       sequence-178338-        6242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human mutant of E2F1 lacking amino acids 193-359.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pcDNA3.1 E2F1 Flag(Delta-193-359) (HindIII- BamHI- 
            ...
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(628..1419,1420..1488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1251..1270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1489..1593)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1668..1687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1859..2238
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2239..2442
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2393..2413
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2487..2506
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2487..2505
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3332..3355
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3419..3437)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3420..3437)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3457..3474)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3463..3687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3733..4161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3820..3839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4030..4051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4170..4190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4175..4504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4355..4490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4417..4436
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4571..5365
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4625..4644)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5235..5254
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5539..5660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5576..5595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5630..5649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5709..5725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5709..5725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5722..5744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5733..5749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5757..5763,5764..5781,5782..5787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5802..5823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5940..5957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6111..6242,1..457))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6191..6210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
       61 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      481 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      541 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      601 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      661 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
      721 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
      781 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
      841 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
      901 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
      961 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1021 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1081 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1141 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1201 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1261 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1321 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1381 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1441 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1501 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1561 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     1621 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     1681 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     1741 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     1801 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     1861 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1921 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1981 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2041 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2101 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2161 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2221 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2281 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2341 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2401 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     2461 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttac
     2521 catggccttg gccggggccc ctgcgggcgg cccatgcgcg ccggcgctgg aggccctgct
     2581 cggggccggc gcgctgcggc tgctcgactc ctcgcagatc gtcatcatct ccgccgcgca
     2641 ggacgccagc gccccgccgg ctcccaccgg ccccgcggcg cccgccgccg gcccctgcga
     2701 ccctgacctg ctgctcttcg ccacaccgca ggcgccccgg cccacaccca gtgcgccgcg
     2761 gcccgcgctc ggccgcccgc cggtgaagcg gaggctggac ctggaaactg accatcagta
     2821 cctggccgag agcagtgggc cagctcgggg cagaggccgc catccaggaa aaggtgtgaa
     2881 atccccgggg gagaagtcac gctatgagac ctcactgaat ctgaccacca agcgcttcct
     2941 ggagctgctg agccactcgg ctgacggtgt cgtcgacctg aactgggctg ccgaggtgct
     3001 gaaggtgcag aagcggcgca tctatgacat caccaacgtc cttgagggca tccagctcat
     3061 tgccaagaag tccaagaacc acatccagtg gctgggatcc cggatgggca gcctgcgggc
     3121 tcccgtggac gaggaccgcc tgtccccgct ggtggcggcc gactcgctcc tggagcatgt
     3181 gcgggaggac ttctccggcc tcctccctga ggagttcatc agcctttccc caccccacga
     3241 ggccctcgac taccacttcg gcctcgagga gggcgagggc atcagagacc tcttcgactg
     3301 tgactttggg gacctcaccc ccctggattt cgattataaa gatgatgatg ataaatgaga
     3361 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc
     3421 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3481 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3541 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3601 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3661 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3721 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3781 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3841 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3901 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3961 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4021 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4081 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4141 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4321 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4441 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4501 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4561 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4621 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4681 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4741 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4801 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4861 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4921 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4981 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5041 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5101 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5161 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5221 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5281 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5341 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5401 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5461 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5521 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5641 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5701 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5761 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5821 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5881 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6241 cc
//
